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Open data
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Basic information
Entry | Database: PDB / ID: 1r3l | ||||||
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Title | potassium channel KcsA-Fab complex in Cs+ | ||||||
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Function / homology | ![]() humoral immune response mediated by circulating immunoglobulin / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhou, Y. / MacKinnon, R. | ||||||
![]() | ![]() Title: The occupancy of ions in the K+ selectivity filter: Charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates Authors: Zhou, Y. / MacKinnon, R. | ||||||
History |
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Remark 600 | HETEROGEN The ligand DGA is a partial lipid. | ||||||
Remark 999 | SEQUENCE No suitable database reference sequence was found for chains A and B at the time of processing. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.3 KB | Display | ![]() |
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PDB format | ![]() | 92.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1r3iC ![]() 1r3jC ![]() 1r3kC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | homo-tetramer of KcsA is generated by four fold axis: x,y,z -x,-y,z -x,y,z x,-y,z |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | ![]() Mass: 13211.582 Da / Num. of mol.: 1 / Mutation: P2A,L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() |
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#2: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() |
-Non-polymers , 4 types, 200 molecules ![](data/chem/img/F09.gif)
![](data/chem/img/CS.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CS.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-F09 / ![]() | ||||
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#5: Chemical | ChemComp-CS / #6: Chemical | ChemComp-DGA / | ![]() #7: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.77 Å3/Da / Density % sol: 67.36 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.4 Details: PEG400, sodium acetate, magnesium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.5 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Apr 25, 2001 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.4→30 Å / Num. all: 34694 / Num. obs: 34694 / % possible obs: 98.8 % / Biso Wilson estimate: 51.2 Å2 |
Reflection shell | Resolution: 2.4→2.5 Å / % possible all: 94.7 |
Reflection | *PLUS Rmerge(I) obs: 0.071 |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() Starting model: 1K4C Resolution: 2.41→26.57 Å / Rfactor Rfree error: 0.006 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: The occupancy of ions in this model were set to 1. Please refer to the primary citation for a detailed analysis of ion occupancy.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 45.1747 Å2 / ksol: 0.330296 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.1 Å2
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Refine analyze | Luzzati coordinate error free: 0.37 Å / Luzzati sigma a free: 0.37 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.41→26.57 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.55 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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