[English] 日本語
Yorodumi- PDB-3or7: On the structural basis of modal gating behavior in K+channels - E71I -
+Open data
-Basic information
Entry | Database: PDB / ID: 3or7 | ||||||
---|---|---|---|---|---|---|---|
Title | On the structural basis of modal gating behavior in K+channels - E71I | ||||||
Components |
| ||||||
Keywords | IMMUNE SYSTEM/TRANSPORT PROTEIN / inactivation / alpha-helical / Potassium channel / IMMUNE SYSTEM-TRANSPORT PROTEIN complex | ||||||
Function / homology | Function and homology information monoatomic ion transmembrane transport / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Chakrapani, S. / Cordero-Morales, J.F. / Jogini, V. / Pan, A.C. / Cortes, D.M. / Roux, B. / Perozo, E. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2011 Title: On the structural basis of modal gating behavior in K(+) channels. Authors: Chakrapani, S. / Cordero-Morales, J.F. / Jogini, V. / Pan, A.C. / Cortes, D.M. / Roux, B. / Perozo, E. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3or7.cif.gz | 109 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3or7.ent.gz | 88.1 KB | Display | PDB format |
PDBx/mmJSON format | 3or7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/or/3or7 ftp://data.pdbj.org/pub/pdb/validation_reports/or/3or7 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||||||||||||||
Unit cell |
| ||||||||||||||||||||||||
Components on special symmetry positions |
|
-Components
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) | ||
---|---|---|---|
#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) | ||
#3: Protein | Mass: 10962.780 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 22-124 / Mutation: E71I Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P0A334 | ||
#4: Chemical | ChemComp-K / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 4.02 Å3/Da / Density % sol: 69.4 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate pH 5.0-6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1 Å |
---|---|
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 13, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40 Å / Num. all: 40893 / Num. obs: 39318 / % possible obs: 96 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
-Processing
Software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.3→40 Å / σ(F): 2
| |||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→40 Å
| |||||||||||||||
Refine LS restraints |
|