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Open data
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Basic information
Entry | Database: PDB / ID: 1qql | ||||||
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Title | THE CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 7/1 CHIMERA | ||||||
![]() | FIBROBLAST GROWTH FACTOR 7/1 CHIMERA | ||||||
![]() | HORMONE/GROWTH FACTOR / BETA-TREFOIL / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | ![]() secretion by lung epithelial cell involved in lung growth / FGFR2b ligand binding and activation / type 2 fibroblast growth factor receptor binding / SHC-mediated cascade:FGFR2 / FRS-mediated FGFR2 signaling / PI-3K cascade:FGFR2 / PI3K Cascade / Phospholipase C-mediated cascade; FGFR2 / PIP3 activates AKT signaling / positive regulation of keratinocyte proliferation ...secretion by lung epithelial cell involved in lung growth / FGFR2b ligand binding and activation / type 2 fibroblast growth factor receptor binding / SHC-mediated cascade:FGFR2 / FRS-mediated FGFR2 signaling / PI-3K cascade:FGFR2 / PI3K Cascade / Phospholipase C-mediated cascade; FGFR2 / PIP3 activates AKT signaling / positive regulation of keratinocyte proliferation / Negative regulation of FGFR2 signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of epithelial cell proliferation involved in lung morphogenesis / positive regulation of myoblast proliferation / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / phosphatidylcholine biosynthetic process / phospholipid biosynthetic process / positive regulation of keratinocyte migration / mesenchymal cell proliferation / branching involved in salivary gland morphogenesis / surfactant homeostasis / protein localization to cell surface / RAF/MAP kinase cascade / myoblast proliferation / endothelial cell proliferation / hair follicle morphogenesis / positive chemotaxis / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ye, S. / Luo, Y. / Pelletier, H. / McKeehan, W.L. | ||||||
![]() | ![]() Title: Structural basis for interaction of FGF-1, FGF-2, and FGF-7 with different heparan sulfate motifs. Authors: Ye, S. / Luo, Y. / Lu, W. / Jones, R.B. / Linhardt, R.J. / Capila, I. / Toida, T. / Kan, M. / Pelletier, H. / McKeehan, W.L. #1: ![]() Title: The glycine box: a determinant of specificity for fibroblast growth factor. Authors: Luo, Y. / Lu, W. / Mohamedali, K.A. / Jang, J.H. / Jones, R.B. / Gabriel, J.L. / Kan, M. / McKeehan, W.L. #2: ![]() Title: Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth. Authors: Finch, P.W. / Rubin, J.S. / Miki, T. / Ron, D. / Aaronson, S.A. #3: ![]() Title: Structure of a heparin-linked biologically active dimer of fibroblast growth factor. Authors: DiGabriele, A.D. / Lax, I. / Chen, D.I. / Svahn, C.M. / Jaye, M. / Schlessinger, J. / Hendrickson, W.A. #4: ![]() Title: Heparin structure and interactions with basic fibroblast growth factor. Authors: Faham, S. / Hileman, R.E. / Fromm, J.R. / Linhardt, R.J. / Rees, D.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.6 KB | Display | ![]() |
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PDB format | ![]() | 30.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16433.945 Da / Num. of mol.: 1 Fragment: DUAL-FUNCTION CHIMERA BETWEEN RAT FGF-7 ENCODED BY EXON 1 AND 2 AND HUMAN FGF-1 ENCODED BY EXON 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() Genus: Rattus, Homo ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.96 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, SODIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 2.3→30 Å / Num. all: 7140 / Num. obs: 7140 / % possible obs: 96.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9 % / Rmerge(I) obs: 0.065 / Net I/σ(I): 28.1 | ||||||||||||||||||||
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 7.8 % / Rmerge(I) obs: 0.299 / % possible all: 95 | ||||||||||||||||||||
Reflection | *PLUS Lowest resolution: 30 Å / Num. measured all: 64157 | ||||||||||||||||||||
Reflection shell | *PLUS % possible obs: 95 % |
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Processing
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Refinement | Resolution: 2.3→6 Å / σ(F): 2 / σ(I): 2
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Refinement step | Cycle: LAST / Resolution: 2.3→6 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 6 Å / σ(F): 2 / Rfactor obs: 0.237 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |