+Open data
-Basic information
Entry | Database: PDB / ID: 1q2h | ||||||
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Title | Phenylalanine Zipper Mediates APS Dimerization | ||||||
Components | adaptor protein with pleckstrin homology and src homology 2 domainsAdapter | ||||||
Keywords | SIGNALING PROTEIN / signal transduction | ||||||
Function / homology | Function and homology information antigen receptor-mediated signaling pathway / transmembrane receptor protein tyrosine kinase adaptor activity / Regulation of KIT signaling / signaling adaptor activity / SH2 domain binding / B cell receptor signaling pathway / blood coagulation / insulin receptor signaling pathway / Factors involved in megakaryocyte development and platelet production / intracellular signal transduction ...antigen receptor-mediated signaling pathway / transmembrane receptor protein tyrosine kinase adaptor activity / Regulation of KIT signaling / signaling adaptor activity / SH2 domain binding / B cell receptor signaling pathway / blood coagulation / insulin receptor signaling pathway / Factors involved in megakaryocyte development and platelet production / intracellular signal transduction / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.7 Å | ||||||
Authors | Dhe-Paganon, S. / Werner, E.D. / Nishi, M. / Chi, Y.-I. / Shoelson, S.E. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2004 Title: A phenylalanine zipper mediates APS dimerization. Authors: Dhe-Paganon, S. / Werner, E.D. / Nishi, M. / Hansen, L. / Chi, Y.-I. / Shoelson, S.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q2h.cif.gz | 48.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q2h.ent.gz | 36.2 KB | Display | PDB format |
PDBx/mmJSON format | 1q2h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q2/1q2h ftp://data.pdbj.org/pub/pdb/validation_reports/q2/1q2h | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 7778.675 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: APS / Plasmid: pET28a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O14492 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.62 % |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6 Details: 0.48-0.68 M sodium iodide, 2.5 % PEG 4K, 0.09 M sodium citrate, 10 mM DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.95000, 0.9174, 0.9211, 0.9208 | |||||||||||||||
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Jan 1, 2002 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→30 Å / Num. obs: 19012 / Observed criterion σ(I): 0 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.7→30 Å / σ(F): 0 / Details: waters picked at level greater than 3.0
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.7→30 Å
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