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Yorodumi- PDB-1pxt: THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLAS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pxt | ||||||
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Title | THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY | ||||||
Components | PEROXISOMAL 3-KETOACYL-COA THIOLASE | ||||||
Keywords | THIOLASE | ||||||
Function / homology | Function and homology information alpha-linolenic acid (ALA) metabolism / Beta-oxidation of very long chain fatty acids / acetyl-CoA C-acyltransferase / Peroxisomal protein import / acetyl-CoA C-acyltransferase activity / phenylacetate catabolic process / fatty acid beta-oxidation / peroxisomal matrix / Neutrophil degranulation / mitochondrial intermembrane space ...alpha-linolenic acid (ALA) metabolism / Beta-oxidation of very long chain fatty acids / acetyl-CoA C-acyltransferase / Peroxisomal protein import / acetyl-CoA C-acyltransferase activity / phenylacetate catabolic process / fatty acid beta-oxidation / peroxisomal matrix / Neutrophil degranulation / mitochondrial intermembrane space / peroxisome / mRNA binding Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | ||||||
Authors | Mathieu, M. / Wierenga, R.K. | ||||||
Citation | Journal: Structure / Year: 1994 Title: The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology. Authors: Mathieu, M. / Zeelen, J.P. / Pauptit, R.A. / Erdmann, R. / Kunau, W.H. / Wierenga, R.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pxt.cif.gz | 136.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pxt.ent.gz | 108.9 KB | Display | PDB format |
PDBx/mmJSON format | 1pxt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/px/1pxt ftp://data.pdbj.org/pub/pdb/validation_reports/px/1pxt | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 41745.715 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) References: UniProt: P27796, acetyl-CoA C-acyltransferase |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.28 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Details: THE PROTEIN HAS BEEN CRYSTALLIZED IN THE ABSENCE OF AN ACTIVE SITE LIGAND. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.4 / Method: microdialysis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 30 Å / Num. obs: 18981 / % possible obs: 92 % / Num. measured all: 47231 / Rmerge(I) obs: 0.079 |
Reflection shell | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 2.9 Å / % possible obs: 92.6 % |
-Processing
Software |
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Refinement | Resolution: 2.8→8 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.198 / Rfactor Rfree: 0.334 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2 |