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Yorodumi- PDB-1pwj: Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism... -
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-Basic information
Entry | Database: PDB / ID: 1pwj | ||||||
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Title | Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of Domain Swapped Dimer Assembly | ||||||
Components | dynein light chain-2 | ||||||
Keywords | CONTRACTILE PROTEIN / Dynein / Dynein Light Chain / Dimer-monomer Equilibrium / Domain swapping | ||||||
Function / homology | Function and homology information Activation of BMF and translocation to mitochondria / Activation of BMF and translocation to mitochondria / Intraflagellar transport / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Resolution of Sister Chromatid Cohesion / COPI-independent Golgi-to-ER retrograde traffic / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / RHO GTPases Activate Formins / Aggrephagy ...Activation of BMF and translocation to mitochondria / Activation of BMF and translocation to mitochondria / Intraflagellar transport / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Resolution of Sister Chromatid Cohesion / COPI-independent Golgi-to-ER retrograde traffic / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / RHO GTPases Activate Formins / Aggrephagy / Separation of Sister Chromatids / Intraflagellar transport / Macroautophagy / COPI-mediated anterograde transport / myosin V complex / COPI-independent Golgi-to-ER retrograde traffic / Macroautophagy / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / 9+0 non-motile cilium / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / Aggrephagy / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / MHC class II antigen presentation / RHO GTPases Activate Formins / Separation of Sister Chromatids / MHC class II antigen presentation / cytoplasmic dynein complex / dynein intermediate chain binding / microtubule-based process / scaffold protein binding / postsynapse / microtubule / postsynaptic density / cytoskeleton / centrosome / glutamatergic synapse / protein-containing complex binding / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Wang, W. / Lo, K.W.-H. / Kan, H.-M. / Fan, J.-S. / Zhang, M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of the Domain-swapped Dimer Assembly Authors: Wang, W. / Lo, K.W.-H. / Kan, H.-M. / Fan, J.-S. / Zhang, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pwj.cif.gz | 558.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pwj.ent.gz | 466.7 KB | Display | PDB format |
PDBx/mmJSON format | 1pwj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pw/1pwj ftp://data.pdbj.org/pub/pdb/validation_reports/pw/1pwj | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10364.847 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Plasmid: pET / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q9D0M5, UniProt: Q78P75*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details |
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Sample conditions | pH: 3.0 / Temperature: 303.3 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |