+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1nnk | ||||||
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タイトル | X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-ATPA at 1.85 A resolution. Crystallization with zinc ions. | ||||||
要素 | Glutamate receptor 2GRIA2 | ||||||
キーワード | MEMBRANE PROTEIN (膜タンパク質) / Ionotropic glutamate receptor GluR2 / ligand-binding core / agonist complex (アゴニスト) | ||||||
機能・相同性 | 機能・相同性情報 spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex ...spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / cellular response to glycine / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / glutamate receptor binding / positive regulation of synaptic transmission / presynaptic active zone membrane / glutamate-gated receptor activity / response to fungicide / regulation of synaptic transmission, glutamatergic / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / dendrite membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / ionotropic glutamate receptor binding / cytoskeletal protein binding / ionotropic glutamate receptor signaling pathway / dendrite cytoplasm / SNARE binding / dendritic shaft / synaptic membrane / synaptic transmission, glutamatergic / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / PDZ domain binding / protein tetramerization / postsynaptic density membrane / Schaffer collateral - CA1 synapse / modulation of chemical synaptic transmission / establishment of protein localization / receptor internalization / terminal bouton / cerebral cortex development / synaptic vesicle membrane / シナプス小胞 / presynapse / presynaptic membrane / signaling receptor activity / amyloid-beta binding / 成長円錐 / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / perikaryon / 樹状突起スパイン / postsynaptic density / neuron projection / 神経繊維 / neuronal cell body / glutamatergic synapse / シナプス / 樹状突起 / protein-containing complex binding / endoplasmic reticulum membrane / protein kinase binding / 細胞膜 / 小胞体 / protein-containing complex / 生体膜 / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Rattus norvegicus (ドブネズミ) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.85 Å | ||||||
データ登録者 | Lunn, M.-L. / Hogner, A. / Stensbol, T.B. / Gouaux, E. / Egebjerg, J. / Kastrup, J.S. | ||||||
引用 | ジャーナル: J.Med.Chem. / 年: 2003 タイトル: Three-Dimensional Structure of the Ligand-Binding Core of GluR2 in Complex with the Agonist (S)-ATPA: Implications for Receptor Subunit Selectivity. 著者: Lunn, M.L. / Hogner, A. / Stensbol, T.B. / Gouaux, E. / Egebjerg, J. / Kastrup, J.S. #1: ジャーナル: J.Mol.Biol. / 年: 2002 タイトル: Structural basis for AMPA receptor activation and ligand selectivity: Crystal structures of five agonist complexes with the GluR2 ligand binding core. 著者: Hogner, A. / Kastrup, J.S. / Jin, R. / Liljefors, T. / Mayer, M.L. / Egebjerg, J. / Larsen, I. / Gouaux, E. #2: ジャーナル: Neuron / 年: 2000 タイトル: Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: Crystal structures of the GluR2 ligand binding core. 著者: Armstrong, N. / Gouaux, E. #3: ジャーナル: Nature / 年: 2002 タイトル: Mechanism of glutamate receptor desensitization. 著者: Sun, Y. / Olson, R. / Horning, M. / Armstrong, N. / Mayer, M. / Gouaux, E. #4: ジャーナル: Protein Sci. / 年: 1998 タイトル: Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable construct. 著者: Chen, G.Q. / Sun, R. / Jin, R. / Gouaux, E. | ||||||
履歴 |
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Remark 300 | BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 1 ... BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 1 CHAIN(S). SEE REMARK 350 FOR INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S). NOTE THAT COORDINATES FOR ONE DIMER OF THE TETRAMERIC MULTIMER REPRESENTING THE KNOWN BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS GIVEN IN REMARK 350. | ||||||
Remark 999 | SEQUENCE Native GluR2 is a membrane protein. The protein crystallized is the extracellular ligand- ...SEQUENCE Native GluR2 is a membrane protein. The protein crystallized is the extracellular ligand-binding core of GluR2. Transmembrane regions were genetically removed and replaced with a Gly-Thr linker (residues 115-116). Therefore, the sequence matches discontinuously with the reference database (413-527, 653-796). The two first residues of the sequence (Gly-2, Ala-1) are cloning artifacts and were not located in the electron density map. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1nnk.cif.gz | 72.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1nnk.ent.gz | 52.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1nnk.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/nn/1nnk ftp://data.pdbj.org/pub/pdb/validation_reports/nn/1nnk | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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詳細 | The biological assembly is a tetramer composed of dimers-of-dimers. Only the dimer is observed in the crystal. The dimer may be generated by applying the following to chain A: TRANSFORM FRACTIONAL - -1.00000 0.00000 0.00000 - 0.00000 -1.00000 0.00000 - 0.00000 0.00000 1.00000 - 2.00000 0.00000 0.00000 |
-要素
#1: タンパク質 | 分子量: 29221.682 Da / 分子数: 1 / 断片: GluR2-flop ligand-binding core (S1S2J) / 由来タイプ: 組換発現 / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / プラスミド: pET30B / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: P19491 | ||||||
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#2: 化合物 | #3: 化合物 | ChemComp-CL / | #4: 化合物 | ChemComp-CE2 / | #5: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.4 Å3/Da / 溶媒含有率: 49 % | ||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 279 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.5 詳細: zinc acetate, cacodylate, PEG8000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K | ||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 6 ℃ | ||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: MAX II / ビームライン: I711 / 波長: 1.0835 Å |
検出器 | タイプ: MARRESEARCH / 検出器: CCD / 日付: 2001年3月14日 |
放射 | モノクロメーター: NULL. / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.0835 Å / 相対比: 1 |
反射 | 解像度: 1.85→20 Å / Num. all: 23016 / Num. obs: 23016 / % possible obs: 92.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 3.5 % / Biso Wilson estimate: 18.2 Å2 / Rmerge(I) obs: 0.099 / Net I/σ(I): 7 |
反射 シェル | 解像度: 1.85→1.92 Å / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 2 / Num. unique all: 1996 / % possible all: 82.1 |
反射 | *PLUS 最低解像度: 20 Å |
反射 シェル | *PLUS % possible obs: 82.1 % / Rmerge(I) obs: 0.34 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: GluR2:(S)-thio-ATPA complex (Lunn et al., to be published). 解像度: 1.85→19.99 Å / Rfactor Rfree error: 0.009 / Data cutoff high rms absF: 1449707.81 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: Engh & Huber 詳細: The first three N-terminal residues and the last two C-terminal residues were not located in the electron density map.
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 48.7733 Å2 / ksol: 0.418009 e/Å3 | ||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 27.4 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 1.85→19.99 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 1.85→1.97 Å / Rfactor Rfree error: 0.029 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top | ||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最低解像度: 20 Å / % reflection Rfree: 2.7 % | ||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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