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- PDB-1mzw: Crystal structure of a U4/U6 snRNP complex between human spliceos... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mzw | ||||||
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Title | Crystal structure of a U4/U6 snRNP complex between human spliceosomal cyclophilin H and a U4/U6-60K peptide | ||||||
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Function / homology | ![]() spliceosomal snRNP complex / U4/U6 snRNP / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Reidt, U. / Wahl, M.C. / Horowitz, D.S. / Luehrmann, R. / Ficner, R. | ||||||
![]() | ![]() Title: Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide Authors: Reidt, U. / Wahl, M.C. / Fasshauer, D. / Horowitz, D.S. / Luehrmann, R. / Ficner, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.6 KB | Display | ![]() |
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PDB format | ![]() | 41.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1qoiS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19230.117 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 3498.044 Da / Num. of mol.: 1 / Fragment: residues 107-137, internal domain / Source method: obtained synthetically / Details: peptide B106-B136 was chemically synthesized / References: UniProt: O43172 |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.88 % | ||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 8000, magnesium acetate, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 21 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 10, 2001 / Details: Osmic Mirrors |
Radiation | Monochromator: Osmic Mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→100 Å / Num. obs: 55492 / % possible obs: 99.2 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Rsym value: 0.063 |
Reflection shell | Resolution: 2→2.1 Å / Rsym value: 0.236 / % possible all: 99 |
Reflection | *PLUS Highest resolution: 2.1 Å / Num. obs: 13762 / Num. measured all: 55492 / Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 2.2 Å / % possible obs: 99 % / Rmerge(I) obs: 0.236 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1QOI Resolution: 2→15 Å / Isotropic thermal model: ISOTROPIC / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→15 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.1 Å / % reflection Rfree: 10 % / Rfactor Rfree![]() | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor Rfree: 0.275 / Rfactor Rwork: 0.212 |