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Yorodumi- PDB-2azn: X-RAY Structure of 2,5-diamino-6-ribosylamino-4(3h)-pyrimidinone ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2azn | ||||||
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Title | X-RAY Structure of 2,5-diamino-6-ribosylamino-4(3h)-pyrimidinone 5-phosphate reductase | ||||||
Components | Putative 5-amino-6-(5-phosphoribosylamino)uracil reductase5-amino-6-(5-phosphoribosylamino)uracil reductase | ||||||
Keywords | OXIDOREDUCTASE | ||||||
Function / homology | Function and homology information 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase / riboflavin biosynthetic process / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / NAD binding / NADP binding / protein dimerization activity Similarity search - Function | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.7 Å | ||||||
Authors | Chatwell, L. / Bacher, A. / Huber, R. / Fischer, M. / Krojer, T. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Biosynthesis of riboflavin: structure and properties of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase of Methanocaldococcus jannaschii Authors: Chatwell, L. / Krojer, T. / Fidler, A. / Eisenreich, W. / Bacher, A. / Huber, R. / Fischer, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2azn.cif.gz | 277 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2azn.ent.gz | 236 KB | Display | PDB format |
PDBx/mmJSON format | 2azn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/az/2azn ftp://data.pdbj.org/pub/pdb/validation_reports/az/2azn | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 24721.162 Da / Num. of mol.: 6 / Fragment: residues 6-224 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Production host: Escherichia coli (E. coli) References: UniProt: Q58085, 5-amino-6-(5-phosphoribosylamino)uracil reductase #2: Chemical | ChemComp-MA5 / #3: Chemical | ChemComp-NAP / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.39 Å3/Da / Density % sol: 63.72 % |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 1.006, 1.009, 0.95 | ||||||||||||
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Detector | Type: MARRESEARCH / Detector: CCD | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.5→25 Å / Num. obs: 134041 |
-Processing
Software | Name: CNS / Classification: refinement | ||||||||||||
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Refinement | Method to determine structure: MAD / Resolution: 2.7→20 Å / σ(F): 1.3
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Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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