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- PDB-1mdw: Crystal Structure of Calcium-Bound Protease Core of Calpain II Re... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mdw | ||||||
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Title | Crystal Structure of Calcium-Bound Protease Core of Calpain II Reveals the Basis for Intrinsic Inactivation | ||||||
![]() | Calpain II, catalytic subunit![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Moldoveanu, T. / Hosfield, C.M. / Lim, D. / Jia, Z. / Davies, P.L. | ||||||
![]() | ![]() Title: Calpain silencing by a reversible intrinsic mechanism. Authors: Moldoveanu, T. / Hosfield, C.M. / Lim, D. / Jia, Z. / Davies, P.L. #1: ![]() Title: A Ca(2+) Switch Aligns the Active Site of Calpain Authors: Moldoveanu, T. / Hosfield, C.M. / Lim, D. / Elce, J.S. / Jia, Z. / Davies, P.L. #2: ![]() Title: Crystal Structure of Calpain Reveals the Structural Basis for Ca(2+)-dependent Protease Activity and a Novel Mode of Enzyme Activation Authors: Hosfield, C.M. / Elce, J.S. / Davies, P.L. / Jia, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 145.6 KB | Display | ![]() |
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PDB format | ![]() | 112.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is the monomer of the dimer in the assymetric unit |
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Components
#1: Protein | ![]() Mass: 36807.980 Da / Num. of mol.: 2 / Fragment: Protease Core Domains I and II (Residues 17-346) / Mutation: C105S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.88 % | |||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 10% PEG6000, 0.1M Sodium Acetate, 30mM calcium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 7, 2001 / Details: Mirrors |
Radiation | Monochromator: Yale Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.95→20 Å / Num. obs: 48591 / % possible obs: 93.9 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.049 / Rsym value: 0.035 / Net I/σ(I): 21.1 |
Reflection shell | Resolution: 1.95→2.05 Å / Rmerge(I) obs: 0.356 / Mean I/σ(I) obs: 3.3 / Num. unique all: 4424 / Rsym value: 0.326 / % possible all: 89.9 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. measured all: 496583 |
Reflection shell | *PLUS % possible obs: 89.9 % / Num. unique obs: 4424 / Num. measured obs: 14861 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Refinement step | Cycle: LAST / Resolution: 1.95→20 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 50 Å / % reflection Rfree: 5 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |