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- PDB-1m70: Crystal structure of oxidized recombinant cytochrome c4 from Pseu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1m70 | ||||||
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Title | Crystal structure of oxidized recombinant cytochrome c4 from Pseudomonas stutzeri | ||||||
![]() | Cytochrome c4 | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Noergaard, A. / Harris, P. / Larsen, S. / Christensen, H.E.M. | ||||||
![]() | ![]() Title: Structural comparison of recombinant Pseudomonas stutzeri cytochrome c4 in two oxidation states Authors: Noergaard, A. / Harris, P. / Larsen, S. / Christensen, H.E.M. #1: ![]() Title: Crystallization and preliminary crystallographic investigations of cytochrome c4 from Pseudomonas stutzeri Authors: Kadziola, A. / Larsen, S. / Christensen, H.M. / Karlsson, J.-J. / Ulstrup, J. #2: ![]() Title: Crystal structure of the dihaem cytochrome c4 from Pseudomonas stutzeri determined at 2.2 A resolution Authors: Kadziola, A. / Larsen, S. #3: ![]() Title: Cytochrome c4 Authors: Andersen, N.H. / Christensen, H.E.M. / Iversen, G. / Noergaard, A. / Scharnagl, C. / Thuesen, M.H. / Ulstrup, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 335.7 KB | Display | ![]() |
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PDB format | ![]() | 275.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1m6zC ![]() 1etpS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19700.062 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-HEC / ![]() #3: Chemical | ChemComp-GOL / | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 30.2 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.6 Details: 0.2M ammonium acetate, 0.1M sodium citrate pH 5.6, 30%(w/v) PEG 4000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 1.25→30 Å / Num. all: 178812 / Num. obs: 175219 / % possible obs: 90.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 | ||||||||||||||||||
Reflection shell | Resolution: 1.25→1.29 Å / % possible all: 77 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB entry 1ETP Resolution: 1.25→30 Å / Num. parameters: 60529 / Num. restraintsaints: 86801 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: Anisotropic refinement. Riding H-atoms introduced.
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Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-228 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 10 / Occupancy sum hydrogen: 5368 / Occupancy sum non hydrogen: 6688 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.25→30 Å
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Refine LS restraints |
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