+Open data
-Basic information
Entry | Database: PDB / ID: 1m08 | ||||||
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Title | Crystal structure of the unbound nuclease domain of ColE7 | ||||||
Components | Colicin E7 | ||||||
Keywords | HYDROLASE / HNH motif / endonuclease / colicin / Zn-binding protein | ||||||
Function / homology | Function and homology information extrachromosomal circular DNA / endonuclease activity / killing of cells of another organism / Hydrolases; Acting on ester bonds / defense response to bacterium / metal ion binding Similarity search - Function | ||||||
Biological species | Escherichia coli str. K12 substr. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Cheng, Y.S. / Hsia, K.C. / Doudeva, L.G. / Chak, K.F. / Yuan, H.S. | ||||||
Citation | Journal: J.mol.biol. / Year: 2002 Title: The Crystal Structure of the Nuclease Domain of Colicin E7 Suggests a Mechanism for Binding to Double-stranded DNA by the H-N-H Endonucleases Authors: Cheng, Y.S. / Hsia, K.C. / Doudeva, L.G. / Chak, K.F. / Yuan, H.S. #1: Journal: Structure / Year: 1999 Title: The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein Authors: Ko, T.P. / Liao, C.C. / Ku, W.Y. / Chak, K.F. / Yuan, H.S. | ||||||
History |
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Remark 300 | BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S) ...BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 2 CHAIN(S). These two chains represent the biological dimer, or two biological monomers. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1m08.cif.gz | 70.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1m08.ent.gz | 51.5 KB | Display | PDB format |
PDBx/mmJSON format | 1m08.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m0/1m08 ftp://data.pdbj.org/pub/pdb/validation_reports/m0/1m08 | HTTPS FTP |
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-Related structure data
Related structure data | 7ceiS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15064.120 Da / Num. of mol.: 2 / Fragment: Nuclease Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli str. K12 substr. (bacteria) Species: Escherichia coli / Strain: W3110 / Gene: COLE7 or CEA / Plasmid: pQE70 / Production host: Escherichia coli (E. coli) / Strain (production host): M15 References: UniProt: Q47112, Hydrolases; Acting on ester bonds #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.5 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: sodium phosphate, Sodium Chloride, zinc chloride, ammonium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Aug 8, 2001 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→40 Å / Num. all: 16335 / Num. obs: 16335 / % possible obs: 95 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 18.3 Å2 / Rsym value: 0.044 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 2.1→2.18 Å / Mean I/σ(I) obs: 7 / Num. unique all: 1591 / Rsym value: 0.17 / % possible all: 92.7 |
Reflection | *PLUS Lowest resolution: 40 Å / % possible obs: 95 % / Num. measured all: 53594 / Rmerge(I) obs: 0.044 |
Reflection shell | *PLUS % possible obs: 92.7 % / Rmerge(I) obs: 0.173 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7CEI Resolution: 2.1→25.61 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 369065.68 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 52.3381 Å2 / ksol: 0.370504 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→25.61 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 50 Å / % reflection Rfree: 10 % / Rfactor Rfree: 0.243 / Rfactor Rwork: 0.185 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Lowest resolution: 2.18 Å |