+Open data
-Basic information
Entry | Database: PDB / ID: 1ls4 | ||||||
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Title | NMR structure of apolipophorin-III from Locusta migratoria | ||||||
Components | Apolipophorin-III | ||||||
Keywords | LIPID TRANSPORT / Helix-bundle / exchangeable apolipoprotein | ||||||
Function / homology | Apolipoprotein / lipid transport / Four Helix Bundle (Hemerythrin (Met), subunit A) / Up-down Bundle / extracellular region / Mainly Alpha / Apolipophorin-3b Function and homology information | ||||||
Biological species | Locusta migratoria (migratory locust) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model type details | minimized average | ||||||
Authors | Fan, D. / Wang, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: NMR solution structure and dynamics of an exchangeable apolipoprotein,locusta migratoria apolipophorin III. Authors: Fan, D. / Zheng, Y. / Yang, D. / Wang, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ls4.cif.gz | 1003.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ls4.ent.gz | 839.3 KB | Display | PDB format |
PDBx/mmJSON format | 1ls4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ls/1ls4 ftp://data.pdbj.org/pub/pdb/validation_reports/ls/1ls4 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19209.256 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Locusta migratoria (migratory locust) / Plasmid: LAPOIII-PET22B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL-21(DE3) / References: UniProt: P10762 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details | Contents: 1 mM apolipophorin-III, 15N/13C uniformly labeled, 200 mM phosphate buffer, 0.5 mM sodium Azide Solvent system: 95% H2O/5% D2O |
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Sample conditions | Ionic strength: 200 mM phosphate / pH: 6.4 / Pressure: 1 atm / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 21 |