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Open data
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Basic information
Entry | Database: PDB / ID: 1lan | ||||||
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Title | LEUCINE AMINOPEPTIDASE COMPLEX WITH L-LEUCINAL | ||||||
![]() | LEUCINE AMINOPEPTIDASE![]() | ||||||
![]() | HYDROLASE (ALPHA-AMINOACYLPEPTIDE) / ![]() ![]() ![]() | ||||||
Function / homology | ![]() cysteinylglycine-S-conjugate dipeptidase / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Straeter, N. / Lipscomb, W.N. | ||||||
![]() | ![]() Title: Two-metal ion mechanism of bovine lens leucine aminopeptidase: active site solvent structure and binding mode of L-leucinal, a gem-diolate transition state analogue, by X-ray crystallography. Authors: Strater, N. / Lipscomb, W.N. #1: ![]() Title: Transition State Analogue L-Leucinephosphonic Acid Bound to Bovine Lens Leucine Aminopeptidase: X-Ray Structure at 1.65 Angstroms Resolution in a New Crystal Form Authors: Straeter, N. / Lipscomb, W.N. #2: ![]() Title: Structure and Mechanism of Bovine Lens Leucine Aminopeptidase Authors: Kim, H. / Lipscomb, W.N. #3: ![]() Title: Re-Refinement of the X-Ray Crystal Structure of Bovine Lens Leucine Aminopeptidase Complexed with Bestatin Authors: Kim, H. / Burley, S.K. / Lipscomb, W.N. #4: ![]() Title: Differentiation and Identification of the Two Catalytic Metal Binding Sites in Bovine Lens Leucine Aminopeptidase by X-Ray Crystallography Authors: Kim, H. / Lipscomb, W.N. #5: ![]() Title: X-Ray Crystallographic Determination of the Structure of Bovine Lens Leucine Aminopeptidase Complexed with Amastatin: Formulation of a Catalytic Mechanism Featuring a Gem-Diolate Transition State Authors: Kim, H. / Lipscomb, W.N. #6: ![]() Title: Structure Determination and Refinement of Bovine Lens Leucine Aminopeptidase and its Complex with Bestatin Authors: Burley, S.K. / David, P.R. / Sweet, R.M. / Taylor, A. / Lipscomb, W.N. #7: ![]() Title: Leucine Aminopeptidase: Bestatin Inhibition and a Model for Enzyme-Catalyzed Peptide Hydrolysis Authors: Burley, S.K. / David, P.R. / Lipscomb, W.N. #8: ![]() Title: Molecular Structure of Leucine Aminopeptidase at 2.7 Angstroms Resolution Authors: Burley, S.K. / David, P.R. / Taylor, A. / Lipscomb, W.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.5 KB | Display | ![]() |
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PDB format | ![]() | 88.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 471 | ||||||||||||||||||
Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 52668.855 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-LEU / | ![]() #4: Chemical | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.85 Å3/Da / Density % sol: 56.85 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 7.8 / Details: pH 7.8 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 123 K |
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Diffraction source | Wavelength: 1.5418 Å |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR / Date: Jun 6, 1995 |
Radiation | Monochromator: SUPPER DOUBLE MIRRORS / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Num. obs: 44837 / % possible obs: 99.3 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.07 |
Reflection | *PLUS Highest resolution: 1.9 Å / Num. measured all: 160257 / Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Resolution: 1.9→7 Å / σ(F): 2
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Displacement parameters | Biso mean: 16.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→7 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 2 Å |