+Open data
-Basic information
Entry | Database: PDB / ID: 1kaa | ||||||
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Title | STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE | ||||||
Components | STAPHYLOCOCCAL NUCLEASEMicrococcal nuclease | ||||||
Keywords | HYDROLASE(PHOSPHORIC DIESTER) | ||||||
Function / homology | Function and homology information endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Hodel, A. / Fox, R.O. | ||||||
Citation | Journal: Protein Sci. / Year: 1993 Title: Stress and strain in staphylococcal nuclease. Authors: Hodel, A. / Kautz, R.A. / Jacobs, M.D. / Fox, R.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kaa.cif.gz | 39.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kaa.ent.gz | 27.1 KB | Display | PDB format |
PDBx/mmJSON format | 1kaa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ka/1kaa ftp://data.pdbj.org/pub/pdb/validation_reports/ka/1kaa | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 117 |
-Components
#1: Protein | Mass: 15478.952 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / References: UniProt: P00644, micrococcal nuclease |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.44 % | |||||||||||||||
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Crystal grow | *PLUS pH: 8.15 / Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 6 Å / Num. obs: 10420 / Rmerge(I) obs: 0.063 |
-Processing
Software |
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Refinement | Highest resolution: 1.9 Å /
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Refinement step | Cycle: LAST / Highest resolution: 1.9 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 6 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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