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- PDB-1jun: NMR STUDY OF C-JUN HOMODIMER -

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Basic information

Entry
Database: PDB / ID: 1jun
TitleNMR STUDY OF C-JUN HOMODIMER
ComponentsC-JUN HOMODIMER
KeywordsTRANSCRIPTION REGULATION / DNA-BINDING REGULATORY PROTEIN / ONCOGENE PROTEIN / TRANSCRIPTION ACTIVATION
Function / homology
Function and homology information


cAMP response element binding / transcription factor AP-1 complex / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / WNT5:FZD7-mediated leishmania damping / SMAD protein signal transduction / positive regulation by host of viral transcription / nuclear chromosome / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models ...cAMP response element binding / transcription factor AP-1 complex / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / WNT5:FZD7-mediated leishmania damping / SMAD protein signal transduction / positive regulation by host of viral transcription / nuclear chromosome / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / Activation of the AP-1 family of transcription factors / ubiquitin-like protein ligase binding / negative regulation of DNA binding / R-SMAD binding / general transcription initiation factor binding / negative regulation by host of viral transcription / positive regulation of vascular associated smooth muscle cell proliferation / cellular response to cadmium ion / response to endoplasmic reticulum stress / GTPase activator activity / transforming growth factor beta receptor signaling pathway / Regulation of PTEN gene transcription / TP53 Regulates Transcription of DNA Repair Genes / FCERI mediated MAPK activation / MAPK6/MAPK4 signaling / euchromatin / Signaling by ALK fusions and activated point mutants / Pre-NOTCH Transcription and Translation / DNA-binding transcription repressor activity, RNA polymerase II-specific / cellular response to reactive oxygen species / positive regulation of miRNA transcription / Activation of anterior HOX genes in hindbrain development during early embryogenesis / RNA polymerase II transcription regulator complex / sequence-specific double-stranded DNA binding / regulation of cell population proliferation / Senescence-Associated Secretory Phenotype (SASP) / DNA-binding transcription activator activity, RNA polymerase II-specific / Oxidative Stress Induced Senescence / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / nucleoplasm / identical protein binding / nucleus
Similarity search - Function
Transcription factor Jun / Jun-like transcription factor / Jun-like transcription factor / bZIP transcription factor / Transcription factor, Skn-1-like, DNA-binding domain superfamily / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #170 / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain superfamily ...Transcription factor Jun / Jun-like transcription factor / Jun-like transcription factor / bZIP transcription factor / Transcription factor, Skn-1-like, DNA-binding domain superfamily / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #170 / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain superfamily / Basic-leucine zipper domain / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Transcription factor Jun
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR
AuthorsJunius, F.K. / O'Donoghue, S.I. / Nilges, M. / King, G.F.
Citation
Journal: J.Biol.Chem. / Year: 1996
Title: High resolution NMR solution structure of the leucine zipper domain of the c-Jun homodimer.
Authors: Junius, F.K. / O'Donoghue, S.I. / Nilges, M. / Weiss, A.S. / King, G.F.
#1: Journal: To be Published
Title: Calculation of Symmetric Multimer Structures from NMR Data Using a Priori Knowledge of the Monomer Structure, Co-Monomer Restraints, and Interface Mapping: The Case of Leucine Zippers
Authors: O'Donoghue, S.I. / King, G.F. / Nilges, M.
#2: Journal: Thesis / Year: 1995
Title: Structural Studies of the Leucine Zipper Domain of the Oncoprotein C-Jun
Authors: Junius, F.K.
#3: Journal: Biochemistry / Year: 1995
Title: Nuclear Magnetic Resonance Characterization of the Jun Leucine Zipper Domain: Unusual Properties of Coiled-Coil Interfacial Polar Residues
Authors: Junius, F.K. / Mackay, J.P. / Bubb, W.A. / Jensen, S.A. / Weiss, A.S. / King, G.F.
#4: Journal: Eur.J.Biochem. / Year: 1993
Title: The Solution Structure of the Leucine Zipper Motif of the Jun Oncoprotein Homodimer
Authors: Junius, F.K. / Weiss, A.S. / King, G.F.
#5: Journal: Protein Eng. / Year: 1993
Title: Determination of the Structure of Symmetric Coiled-Coil Proteins from NMR Data: Application of the Leucine Zipper Proteins Jun and GCN4
Authors: O'Donoghue, S.I. / Junius, F.K. / King, G.F.
#6: Journal: Science / Year: 1989
Title: Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun
Authors: O'Shea, E.K. / Rutkowski, R. / Stafford III, W.F. / Kim, P.S.
History
DepositionDec 19, 1995Processing site: BNL
Revision 1.0Jun 20, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: C-JUN HOMODIMER
B: C-JUN HOMODIMER


Theoretical massNumber of molelcules
Total (without water)9,7672
Polymers9,7672
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)7 / -
Representative

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Components

#1: Protein/peptide C-JUN HOMODIMER / JUNLZ / JUNP1N


Mass: 4883.714 Da / Num. of mol.: 2 / Fragment: LEUCINE ZIPPER DOMAIN, RESIDUES 272 - 315 / Mutation: INS(272-275), INS(315)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P05412

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Sample conditionspH: 3.6 / Temperature: 310 K
Crystal grow
*PLUS
Method: other

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 7

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