+
Open data
-
Basic information
Entry | Database: PDB / ID: 1i2g | ||||||
---|---|---|---|---|---|---|---|
Title | Ribonuclease T1 V16T mutant | ||||||
![]() | GUANYL-SPECIFIC RIBONUCLEASE T1 | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() hyphal tip / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | De Vos, S. / Backmann, J. / Steyaert, J. / Loris, R. | ||||||
![]() | ![]() Title: Hydrophobic core manipulations in ribonuclease T1 Authors: De Vos, S. / Backmann, J. / Prevost, M. / Steyaert, J. / Loris, R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 31.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 22.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1fysC ![]() 1fzuC ![]() 1g02C ![]() 1i2eC ![]() 1i2fC ![]() 1i3fC ![]() 1i3iC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 11096.668 Da / Num. of mol.: 1 / Mutation: V16T, Q25K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||||
---|---|---|---|---|---|
#2: Chemical | #3: Chemical | ChemComp-2GP / | ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.17 % | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.2 Details: MPD, calcium chloride, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Zegers, I., (1998) Nat.Struct.Biol., 5, 280. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 293 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 26, 2000 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.85→34 Å / Num. all: 8425 / Num. obs: 8425 / % possible obs: 95.5 % / Observed criterion σ(F): -6 / Observed criterion σ(I): -3 / Redundancy: 5.44 % / Biso Wilson estimate: 10 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 18.79 |
Reflection shell | Resolution: 1.85→1.92 Å / Redundancy: 4.41 % / Rmerge(I) obs: 0.159 / Mean I/σ(I) obs: 8.65 / Num. unique all: 791 / % possible all: 92 |
Reflection | *PLUS Num. measured all: 45840 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.85→34 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 34 Å / σ(F): 0 / Rfactor obs: 0.163 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
|