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Open data
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Basic information
Entry | Database: PDB / ID: 1ht9 | ||||||
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Title | DOMAIN SWAPPING EF-HANDS | ||||||
![]() | CALBINDIN D9K | ||||||
![]() | METAL BINDING PROTEIN / DOMAIN SWAPPING / CALBINDIN D9K / ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hakansson, M. / Svensson, A.L. / Fast, J. / Linse, S. | ||||||
![]() | ![]() Title: An extended hydrophobic core induces EF-hand swapping. Authors: Hakansson, M. / Svensson, A. / Fast, J. / Linse, S. #1: ![]() Title: Symmetric Stabilization of Bound Ca2+ Ions Through Hydrogen Bonding of Coordinating Water Molecules. Ca2+ Binding Abd Structural Stability of Calbindin D9K Mutants Authors: Fast, J. / Muranyi, A. / Gippert, G.P. / Thulin, E. / Evenas, J. / Linse, S. / Hakansson, M. / Svensson, A.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.9 KB | Display | ![]() |
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PDB format | ![]() | 31.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4icbS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 8661.807 Da / Num. of mol.: 2 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.87 Å3/Da / Density % sol: 34.25 % | ||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 293 K / Method: evaporation Details: ammonium sulphate, calcium chloride, magnesium chloride, EVAPORATION, temperature 293K | ||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 9, 1997 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.75→44 Å / Num. obs: 50352 / % possible obs: 91.5 % / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 18 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 14.5 |
Reflection shell | Resolution: 1.75→1.8 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.311 / Mean I/σ(I) obs: 3.7 / % possible all: 60 |
Reflection | *PLUS Lowest resolution: 44 Å / Num. obs: 11923 / Num. measured all: 50352 |
Reflection shell | *PLUS % possible obs: 60.1 % |
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Processing
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Refinement | Method to determine structure![]() ![]() ![]() Starting model: PDB ENTRY 4ICB Resolution: 1.76→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 23.9 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.76→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 5 % / Rfactor obs: 0.162 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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