+Open data
-Basic information
Entry | Database: PDB / ID: 1hml | ||||||
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Title | ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE | ||||||
Components | ALPHA-LACTALBUMIN | ||||||
Keywords | CALCIUM-BINDING PROTEIN | ||||||
Function / homology | Function and homology information Lactose synthesis / lactose synthase activity / lactose biosynthetic process / Golgi lumen / cell-cell signaling / lysozyme activity / defense response to bacterium / Golgi membrane / apoptotic process / calcium ion binding ...Lactose synthesis / lactose synthase activity / lactose biosynthetic process / Golgi lumen / cell-cell signaling / lysozyme activity / defense response to bacterium / Golgi membrane / apoptotic process / calcium ion binding / signal transduction / protein-containing complex / extracellular space Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.7 Å | ||||||
Authors | Ren, J. / Stuart, D.I. / Acharya, K.R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1993 Title: Alpha-lactalbumin possesses a distinct zinc binding site. Authors: Ren, J. / Stuart, D.I. / Acharya, K.R. #1: Journal: J.Mol.Biol. / Year: 1991 Title: Crystal Structure of Human Alpha_Lactalbumin at 7 1.7 Angstroms Resolution Authors: Acharya, K.R. / Ren, J. / Stuart, D.I. / Phillips, D.C. / Fenna, R.F. #2: Journal: Nature / Year: 1986 Title: Alpha_Lactalbumin Possesses a Novel Calcium Binding Loop Authors: Stuart, D.I. / Acharya, K.R. / Walker, N.P.C. / Smith, S.G. / Lewis, M. / Phillips, D.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hml.cif.gz | 40.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hml.ent.gz | 26.8 KB | Display | PDB format |
PDBx/mmJSON format | 1hml.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hm/1hml ftp://data.pdbj.org/pub/pdb/validation_reports/hm/1hml | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16240.864 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P00709, lactose synthase |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-ZN / |
#4: Chemical | ChemComp-SO4 / |
#5: Water | ChemComp-HOH / |
Nonpolymer details | THE MOLECULE ALSO BINDS A SULFATE ION. THE ZINC BINDING SITE ALSO INCLUDES RESIDUE GLU 116 FROM A ...THE MOLECULE ALSO BINDS A SULFATE ION. THE ZINC BINDING SITE ALSO INCLUDES RESIDUE GLU 116 FROM A SYMMETRY-RELATED MOLECULE. |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 30.3 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 35 ℃ / pH: 7.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 10697 / % possible obs: 83.4 % / Observed criterion σ(I): 0 |
-Processing
Software |
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Refinement | Resolution: 1.7→30 Å / σ(F): 0
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Displacement parameters | Biso mean: 19 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→30 Å
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Refine LS restraints |
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