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Yorodumi- PDB-1hlo: THE CRYSTAL STRUCTURE OF AN INTACT HUMAN MAX-DNA COMPLEX: NEW INS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hlo | ||||||
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Title | THE CRYSTAL STRUCTURE OF AN INTACT HUMAN MAX-DNA COMPLEX: NEW INSIGHTS INTO MECHANISMS OF TRANSCRIPTIONAL CONTROL | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / TRANSCRIPTIONAL REGULATION / DNA BINDING / COMPLEX (TRANSCRIPTION FACTOR MAX-DNA) / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | Function and homology information Mad-Max complex / Myc-Max complex / Transcription of E2F targets under negative control by DREAM complex / E-box binding / Transcriptional Regulation by E2F6 / MLL1 complex / Cyclin E associated events during G1/S transition / Cyclin A:Cdk2-associated events at S phase entry / protein-DNA complex / DNA-binding transcription repressor activity, RNA polymerase II-specific ...Mad-Max complex / Myc-Max complex / Transcription of E2F targets under negative control by DREAM complex / E-box binding / Transcriptional Regulation by E2F6 / MLL1 complex / Cyclin E associated events during G1/S transition / Cyclin A:Cdk2-associated events at S phase entry / protein-DNA complex / DNA-binding transcription repressor activity, RNA polymerase II-specific / RNA polymerase II transcription regulator complex / sequence-specific double-stranded DNA binding / DNA-binding transcription factor binding / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / DNA-binding transcription factor activity / dendrite / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | ||||||
Authors | Brownlie, P. / Ceska, T.A. / Lamers, M. / Romier, C. / Theo, H. / Suck, D. | ||||||
Citation | Journal: Structure / Year: 1997 Title: The crystal structure of an intact human Max-DNA complex: new insights into mechanisms of transcriptional control. Authors: Brownlie, P. / Ceska, T. / Lamers, M. / Romier, C. / Stier, G. / Teo, H. / Suck, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hlo.cif.gz | 55.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hlo.ent.gz | 39.5 KB | Display | PDB format |
PDBx/mmJSON format | 1hlo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hl/1hlo ftp://data.pdbj.org/pub/pdb/validation_reports/hl/1hlo | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3334.186 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
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#2: DNA chain | Mass: 3374.210 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
#3: Protein | Mass: 9542.478 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P61244 #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.14 Å3/Da / Density % sol: 70.32 % | ||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||
Components of the solutions |
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Crystal | *PLUS Density % sol: 53 % | ||||||||||||||||||||
Crystal grow | *PLUS PH range low: 4 / PH range high: 3.9 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 2.8 Å / % possible obs: 96 % / Rsym value: 0.098 |
Reflection | *PLUS Highest resolution: 2.8 Å / Rmerge(I) obs: 0.098 |
-Processing
Software |
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Refinement | Resolution: 2.8→8 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 8 Å / Num. reflection all: 10256 / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |