hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds / Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In linear amides / polysaccharide binding / endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process Similarity search - Function
Immunoglobulin-like - #290 / NodB homology domain profile. / NodB homology domain / Polysaccharide deacetylase / Carbohydrate-binding type-2 domain / CBM2 (Carbohydrate-binding type-2) domain profile. / CBD_II / CBM2, carbohydrate-binding domain superfamily / Glycoside hydrolase family 11, active site 2 / Glycosyl hydrolases family 11 (GH11) active site signature 2. ...Immunoglobulin-like - #290 / NodB homology domain profile. / NodB homology domain / Polysaccharide deacetylase / Carbohydrate-binding type-2 domain / CBM2 (Carbohydrate-binding type-2) domain profile. / CBD_II / CBM2, carbohydrate-binding domain superfamily / Glycoside hydrolase family 11, active site 2 / Glycosyl hydrolases family 11 (GH11) active site signature 2. / Glycoside hydrolase family 11, active site 1 / Glycosyl hydrolases family 11 (GH11) active site signature 1. / Glycoside hydrolase family 11 / Glycosyl hydrolases family 11 (GH11) domain / Glycosyl hydrolases family 11 / Glycosyl hydrolases family 11 (GH11) domain profile. / Glycoside hydrolase/deacetylase, beta/alpha-barrel / Glycoside hydrolase family 11/12 / CBM2/CBM3, carbohydrate-binding domain superfamily / Twin arginine translocation (Tat) signal profile. / Twin-arginine translocation pathway, signal sequence / Concanavalin A-like lectin/glucanase domain superfamily / Immunoglobulin-like / Sandwich / Mainly Beta Similarity search - Domain/homology
ENDO-1,4-BETA-XYLANASED / XYLANASE D / CBM2B-2 / XBD2
Mass: 8750.339 Da / Num. of mol.: 1 / Fragment: XYLAN BINDING DOMAIN 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CELLULOMONAS FIMI (bacteria) / Strain: JM83 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P54865, endo-1,4-beta-xylanase
Compound details
ENDOHYDROLYSIS OF 1,4-BETA-D-XYLOSIDIC LINKAGES IN XYLANS. BELONGS TO CELLULASE FAMILY G (FAMILY 11 ...ENDOHYDROLYSIS OF 1,4-BETA-D-XYLOSIDIC LINKAGES IN XYLANS. BELONGS TO CELLULASE FAMILY G (FAMILY 11 OF GLYCOSYL HYDROLASES).
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
TOCSY
1
2
1
DQF-COSY
1
3
1
NOESY
1
4
1
E.COSY
1
5
1
HSQC
1
6
1
NOESY-HMQC
1
7
1
TOCSY-HMQC
1
8
1
HNHA
1
9
1
HNHB
NMR details
Text: MINIMISED AVERAGE FROM BEST 23 OUT OF 50. THE STRUCTURE WAS DETERMINED USING HETERONUCLEAR NMR SPECTROSCOPY ON A UNIFORMLY 15N-LABELLED SAMPLE OF XBD2
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Sample preparation
Details
Contents: SODIUM PHOSPHATE 50 MM, SODIUM AZIDE 10 MM
Sample conditions
pH: 5.0 / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
500
1
Bruker DRX
Bruker
DRX
600
2
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Processing
NMR software
Name
Version
Developer
Classification
X-PLOR
3.1
BRUNGER
refinement
Felix
97
structuresolution
Refinement
Method: HYBRID DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 / Details: YASAP PROTOCOL. DETAILS IN THE JRNL CITATION.
NMR ensemble
Conformers calculated total number: 50 / Conformers submitted total number: 1
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