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- PDB-1h4y: Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unph... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h4y | ||||||
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Title | Structure of the Anti-Sigma Factor Antagonist SpoIIAA in its Unphosphorylated Form | ||||||
![]() | ANTI-SIGMA F FACTOR ANTAGONIST | ||||||
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Function / homology | ![]() anti-sigma factor antagonist activity / antisigma factor binding / sporulation resulting in formation of a cellular spore Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
![]() | ![]() Title: Structure of the Bacillus Cell Fate Determinant Spoiiaa in Phosphorylated and Unphosphorylated Forms Authors: Seavers, P.R. / Lewis, R.J. / Brannigan, J.A. / Verschueren, K.H.G. / Murshudov, G.N. / Wilkinson, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.1 KB | Display | ![]() |
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PDB format | ![]() | 84.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13093.158 Da / Num. of mol.: 2 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() Compound details | CHAIN A, B ENGINEERED | Sequence details | THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUSS STRAIN ATCC14577, IS SIMILAR TO THE SWISSPROT ENTRY ...THE SEQUENCE SPOIIA FROM BACILLUS SPHAERICUS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.41 % Description: ABOVE DATA IS THE NATIVE DATA TO 1.61A. THE STRUCTURE WAS ORIGINALLY SOLVED BY MAD TO 2.5A. | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 8.5 / Details: 15% PEG 4K, 200MM MGCL2, 0.1M TRIS (PH 8.5) | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: Seavers, P.R., (2001) Acta Crystallogr., D57, 292. | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.61→19.9 Å / Num. obs: 25927 / % possible obs: 96.4 % / Redundancy: 4.57 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 19.8 |
Reflection shell | Resolution: 1.61→1.67 Å / Rmerge(I) obs: 0.24 / Mean I/σ(I) obs: 5.4 / % possible all: 87.1 |
Reflection | *PLUS Num. measured all: 118510 |
Reflection shell | *PLUS % possible obs: 87.1 % |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Refinement step | Cycle: LAST / Resolution: 1.61→20 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.159 | ||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||
Refine LS restraints | *PLUS
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