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Yorodumi- PDB-1fbp: CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH F... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fbp | ||||||
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Title | CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH FRUCTOSE 6-PHOSPHATE, AMP, AND MAGNESIUM | ||||||
Components | FRUCTOSE 1,6-BISPHOSPHATASE | ||||||
Keywords | HYDROLASE (PHOSPHORIC MONOESTER) | ||||||
Function / homology | Function and homology information Gluconeogenesis / sucrose biosynthetic process / fructose-bisphosphatase / fructose 1,6-bisphosphate 1-phosphatase activity / negative regulation of Ras protein signal transduction / fructose 1,6-bisphosphate metabolic process / cellular response to magnesium ion / fructose 6-phosphate metabolic process / fructose metabolic process / monosaccharide binding ...Gluconeogenesis / sucrose biosynthetic process / fructose-bisphosphatase / fructose 1,6-bisphosphate 1-phosphatase activity / negative regulation of Ras protein signal transduction / fructose 1,6-bisphosphate metabolic process / cellular response to magnesium ion / fructose 6-phosphate metabolic process / fructose metabolic process / monosaccharide binding / negative regulation of glycolytic process / regulation of gluconeogenesis / AMP binding / dephosphorylation / gluconeogenesis / negative regulation of cell growth / cellular response to xenobiotic stimulus / RNA polymerase II-specific DNA-binding transcription factor binding / negative regulation of transcription by RNA polymerase II / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Ke, H. / Zhang, Y. / Lipscomb, W.N. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1990 Title: Crystal structure of fructose-1,6-bisphosphatase complexed with fructose 6-phosphate, AMP, and magnesium. Authors: Ke, H.M. / Zhang, Y.P. / Lipscomb, W.N. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1991 Title: Crystal Structure of the Neutral Form of Fructose-1,6-Bisphosphatase Complexed with the Product Fructose 6-Phosphate at 2.1-Angstroms Resolution Authors: Ke, H. / Zhang, Y. / Liang, J.-Y. / Lipscomb, W.N. #2: Journal: J.Mol.Biol. / Year: 1989 Title: Structure Refinement of Fructose-1,6-Bisphosphatase and its Fructose 2,6-Bisphosphate Complex at 2.8 Angstroms Resolution Authors: Ke, H. / Thorpe, C.M. / Seaton, B.A. / Lipscomb, W.N. / Marcus, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fbp.cif.gz | 150.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fbp.ent.gz | 123.6 KB | Display | PDB format |
PDBx/mmJSON format | 1fbp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fb/1fbp ftp://data.pdbj.org/pub/pdb/validation_reports/fb/1fbp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36503.004 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / References: UniProt: P00636, fructose-bisphosphatase #2: Sugar | #3: Chemical | #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.23 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.4 / Method: microdialysis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 27511 / Num. measured all: 87690 / Rmerge(I) obs: 0.061 |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||
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Refinement | Rfactor Rwork: 0.215 / Highest resolution: 2.5 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 10 Å / Num. reflection obs: 25402 / Rfactor obs: 0.215 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |