+Open data
-Basic information
Entry | Database: PDB / ID: 1f68 | ||||||
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Title | NMR SOLUTION STRUCTURE OF THE BROMODOMAIN FROM HUMAN GCN5 | ||||||
Components | HISTONE ACETYLTRANSFERASE | ||||||
Keywords | TRANSFERASE / LEFT-HANDED FOUR-HELIX BUNDLE | ||||||
Function / homology | Function and homology information histone succinyltransferase activity / peptidyl-lysine glutarylation / histone glutaryltransferase activity / metencephalon development / regulation of cartilage development / positive regulation of cell projection organization / : / histone H4K12 acetyltransferase activity / histone H3K9 acetyltransferase activity / positive regulation of cardiac muscle cell differentiation ...histone succinyltransferase activity / peptidyl-lysine glutarylation / histone glutaryltransferase activity / metencephalon development / regulation of cartilage development / positive regulation of cell projection organization / : / histone H4K12 acetyltransferase activity / histone H3K9 acetyltransferase activity / positive regulation of cardiac muscle cell differentiation / regulation of stem cell population maintenance / regulation of bone development / regulation of regulatory T cell differentiation / negative regulation of centriole replication / transcription factor TFTC complex / telencephalon development / histone H3 acetyltransferase activity / internal peptidyl-lysine acetylation / histone H3K18 acetyltransferase activity / ATAC complex / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / Cardiogenesis / SAGA complex / RUNX3 regulates NOTCH signaling / limb development / NOTCH4 Intracellular Domain Regulates Transcription / regulation of T cell activation / NOTCH3 Intracellular Domain Regulates Transcription / regulation of tubulin deacetylation / peptide-lysine-N-acetyltransferase activity / midbrain development / intracellular distribution of mitochondria / Notch-HLH transcription pathway / Formation of WDR5-containing histone-modifying complexes / regulation of cell division / Formation of paraxial mesoderm / regulation of RNA splicing / RNA Polymerase I Transcription Initiation / regulation of embryonic development / histone acetyltransferase complex / negative regulation of gluconeogenesis / long-term memory / regulation of DNA repair / somitogenesis / histone acetyltransferase activity / positive regulation of gluconeogenesis / histone acetyltransferase / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / response to nutrient levels / cellular response to nerve growth factor stimulus / neural tube closure / gluconeogenesis / positive regulation of cytokine production / regulation of synaptic plasticity / multicellular organism growth / regulation of protein stability / B-WICH complex positively regulates rRNA expression / response to organic cyclic compound / NOTCH1 Intracellular Domain Regulates Transcription / mitotic spindle / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / histone deacetylase binding / cellular response to tumor necrosis factor / heart development / HATs acetylate histones / fibroblast proliferation / protein phosphatase binding / DNA-binding transcription factor binding / in utero embryonic development / transcription coactivator activity / regulation of cell cycle / Ub-specific processing proteases / chromatin remodeling / centrosome / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / extracellular space / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / Torsion angle dynamics (DYANA) for generation of initial structures; Structure-mediated assignment of ambiguous experimental restraints (SANE); Iteration between DYANA, SANE; Simulated Annealing, Energy Minimization in AMBER using full restraint set. | ||||||
Authors | Wright, P.E. / Hudson, B.P. / Dyson, H.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Solution structure and acetyl-lysine binding activity of the GCN5 bromodomain. Authors: Hudson, B.P. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f68.cif.gz | 1001.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f68.ent.gz | 850.8 KB | Display | PDB format |
PDBx/mmJSON format | 1f68.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f6/1f68 ftp://data.pdbj.org/pub/pdb/validation_reports/f6/1f68 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12257.139 Da / Num. of mol.: 1 / Fragment: BROMODOMAIN / Mutation: P730G Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Organ: LIVER / Plasmid: PET24A / Production host: Escherichia coli (E. coli) / References: UniProt: Q92830, histone acetyltransferase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: Backbone assignments from HNCACB & CBCA(CO)NH (sample 2); Sidechain assignments from C(CO)NH-TOCSY & H(CCO)NH-TOCSY (sample 2); Stereospecific assignments from 13C-{13CO} & 13C-{15N} spin-echo ...Text: Backbone assignments from HNCACB & CBCA(CO)NH (sample 2); Sidechain assignments from C(CO)NH-TOCSY & H(CCO)NH-TOCSY (sample 2); Stereospecific assignments from 13C-{13CO} & 13C-{15N} spin-echo difference CT-HSQC (sample 3); Aromatic assignments from (HB)CB(CGCD)HD, (HB)CB(CGCDCE)HE, & (HC)C(C)CH-TOCSY (sample 3) |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Torsion angle dynamics (DYANA) for generation of initial structures; Structure-mediated assignment of ambiguous experimental restraints (SANE); Iteration between DYANA, SANE; Simulated ...Method: Torsion angle dynamics (DYANA) for generation of initial structures; Structure-mediated assignment of ambiguous experimental restraints (SANE); Iteration between DYANA, SANE; Simulated Annealing, Energy Minimization in AMBER using full restraint set. Software ordinal: 1 Details: Structures are based upon 2232 NOE-derived distance restraints (365 long-range, 379 medium-range, 403 sequential, 1085 intraresidue, and 426 ambiguous), 155 torsion angle restraints, and 47 ...Details: Structures are based upon 2232 NOE-derived distance restraints (365 long-range, 379 medium-range, 403 sequential, 1085 intraresidue, and 426 ambiguous), 155 torsion angle restraints, and 47 stereospecific assignments. | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: NO DISTANCE OR ANGLE VIOLATIONS GREATER THAN 0.15 A OR 5 DEGREES Conformers calculated total number: 185 / Conformers submitted total number: 30 |