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Yorodumi- PDB-1e9r: Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal for... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1e9r | ||||||
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Title | Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal form in complex with sulphate. | ||||||
Components | CONJUGAL TRANSFER PROTEIN TRWB | ||||||
Keywords | COUPLING PROTEIN / BACTERIAL CONJUGATION / F1-ATPASE-LIKE QUATERNARY STRUCTURE / RING HELICASES | ||||||
Function / homology | Function and homology information | ||||||
Biological species | ESCHERICHIA COLI (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.4 Å | ||||||
Authors | Gomis-Rueth, F.X. / Moncalian, G. / Cabezon, E. / de la Cruz, F. / Coll, M. | ||||||
Citation | Journal: Nature / Year: 2001 Title: The Bacterial Conjugation Protein Trwb Resembles Ring Helicases and F1-ATPase Authors: Gomis-Rueth, F.X. / Moncalian, G. / Perez-Luque, R. / Gonzalez, A. / Cabezon, E. / De La Cruz, F. / Coll, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e9r.cif.gz | 500.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e9r.ent.gz | 414.8 KB | Display | PDB format |
PDBx/mmJSON format | 1e9r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e9/1e9r ftp://data.pdbj.org/pub/pdb/validation_reports/e9/1e9r | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given / Matrix: (1), |
-Components
#1: Protein | Mass: 48671.469 Da / Num. of mol.: 6 / Fragment: TRANSMEMBRANE-ANCHOR-EXCISED PROTEIN / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ESCHERICHIA COLI (E. coli) / Gene: TRWB / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q04230 #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | Compound details | CHAIN A, B, D, E, F, G FIRST 70 RESIDUES DELETED | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.9 % | ||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: 1.5 M AMMONIUM SULPHATE, 0.1 M NACL, 0.1 M HEPES PH 7.5 | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.844 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.844 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→72.6 Å / Num. obs: 1205165 / % possible obs: 100 % / Redundancy: 9 % / Rmerge(I) obs: 0.057 / Net I/σ(I): 9 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.4→50 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.21 / Rfactor Rwork: 0.21 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 43 Å2 |