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Structure paper

TitleThe Bacterial Conjugation Protein Trwb Resembles Ring Helicases and F1-ATPase
Journal, issue, pagesNature, Vol. 409, Page 637-, Year 2001
Publish dateOct 26, 2000 (structure data deposition date)
AuthorsGomis-Rueth, F.X. / Moncalian, G. / Perez-Luque, R. / Gonzalez, A. / Cabezon, E. / De La Cruz, F. / Coll, M.
External linksNature / PubMed:11214325
MethodsX-ray diffraction
Resolution2.4 - 3 Å
Structure data

PDB-1e9r:
Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal form in complex with sulphate.
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1e9s:
Bacterial conjugative coupling protein TrwBdeltaN70. Unbound monoclinic form.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-1gki:
Plasmid coupling protein TrwB in complex with ADP and Mg2+.
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-1gl6:
Plasmid coupling protein TrwB in complex with the non-hydrolysable GTP analogue GDPNP
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-1gl7:
Plasmid coupling protein TrwB in complex with the non-hydrolisable ATP-analogue ADPNP.
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM / HEPES

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

ChemComp-CL:
Unknown entry / Chloride

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • escherichia coli (E. coli)
KeywordsCOUPLING PROTEIN / BACTERIAL CONJUGATION / F1-ATPASE-LIKE QUATERNARY STRUCTURE / RING HELICASES / TYPE IV SECRETION SYSTEM / BACTERIAL CONJUG PROTEIN / RING HELICASE / TYPE IV SECRETION SYSTEM CONJUGATIVE COUPLING PROTEIN FROM PLASMID

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