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- PDB-1dxs: Crystal structure of the C-terminal sterile alpha motif (SAM) dom... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1dxs | ||||||
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Title | Crystal structure of the C-terminal sterile alpha motif (SAM) domain of human p73 alpha splice variant | ||||||
![]() | P53-LIKE TRANSCRIPTION FACTOR | ||||||
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Function / homology | ![]() positive regulation of lung ciliated cell differentiation / negative regulation of cardiac muscle cell proliferation / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / Regulation of TP53 Activity through Association with Co-factors / positive regulation of oligodendrocyte differentiation / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / TP53 Regulates Transcription of Caspase Activators and Caspases / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / negative regulation of neuron differentiation ...positive regulation of lung ciliated cell differentiation / negative regulation of cardiac muscle cell proliferation / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / Regulation of TP53 Activity through Association with Co-factors / positive regulation of oligodendrocyte differentiation / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / TP53 Regulates Transcription of Caspase Activators and Caspases / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / negative regulation of neuron differentiation / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wang, W.K. / Chen, Y.W. | ||||||
![]() | ![]() Title: Structure of the C-Terminal Sterile Alpha-Motif (Sam) Domain of Human P73 Alpha Authors: Wang, W.K. / Bycroft, M. / Foster, N.W. / Buckle, A.M. / Fersht, A.R. / Chen, Y.W. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Crystallization and Preliminary Crystallographic Studies of a Sam Domain at the C-Terminus of Human P73 Alpha Authors: Wang, W.K. / Proctor, M. / Buckle, A.M. / Bycroft, M. / Chen, Y.W. #2: ![]() Title: Solution Structure of a Conserved C-Terminal Domain of P73 with Structural Homology to the Sam Domain Authors: Chi, S.-W. / Ayed, A. / Arrowsmith, C.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 23.7 KB | Display | ![]() |
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PDB format | ![]() | 14.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1cokS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | BIOLOGICAL_UNIT: MONOMER |
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Components
#1: Protein | Mass: 9026.124 Da / Num. of mol.: 1 / Fragment: C-TERMINAL STERILE ALPHA MOTIF (SAM) DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
Sequence details | RESIDUES GLY-SER (-2 TO -1) CLONING ARTIFACT |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36 % | ||||||||||||||||||||
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Crystal grow![]() | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: CRYSTALLIZED IN 0.1M TRIS-HCL PH8.5, 2.0M MONO-AMMONIUM DIHYDROGEN PHOSPHATE, AT 290K., pH 8.50 | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 290 K / Method: vapor diffusion, hanging dropDetails: Wang, W.K., (2000) Acta Crystallogr.,Sect.D, 56, 769. | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 15, 1999 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.54→26 Å / Num. obs: 2510 / % possible obs: 94.9 % / Observed criterion σ(I): 2 / Redundancy: 6.8 % / Biso Wilson estimate: 59.4 Å2 / Rmerge(I) obs: 0.073 / Rsym value: 0.067 / Net I/σ(I): 7.6 |
Reflection shell | Resolution: 2.54→2.71 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.408 / Mean I/σ(I) obs: 2 / Rsym value: 0.377 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1COK Resolution: 2.54→26 Å / Isotropic thermal model: UNRESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: C TERMINAL RESIDUES ARE NOT SEEN IN THE DENSITY MAPS
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Solvent computation | Solvent model: MASKED FLAT MODEL / Bsol: 92.7 Å2 / ksol: 0.352 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.54→26 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.54→2.63 Å / Total num. of bins used: 10
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9A / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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