+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1duj | ||||||
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タイトル | SOLUTION STRUCTURE OF THE SPINDLE ASSEMBLY CHECKPOINT PROTEIN HUMAN MAD2 | ||||||
要素 | SPINDLE ASSEMBLY CHECKPOINT PROTEIN紡錘体チェックポイント | ||||||
キーワード | CELL CYCLE (細胞周期) / Mad2 (Mad2) / spindle assembly checkpoint (紡錘体チェックポイント) | ||||||
機能・相同性 | 機能・相同性情報 mitotic spindle assembly checkpoint MAD1-MAD2 complex / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / mitotic checkpoint complex / positive regulation of mitotic cell cycle spindle assembly checkpoint / establishment of centrosome localization / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / nuclear pore nuclear basket / negative regulation of ubiquitin protein ligase activity / mitotic spindle assembly checkpoint signaling ...mitotic spindle assembly checkpoint MAD1-MAD2 complex / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / mitotic checkpoint complex / positive regulation of mitotic cell cycle spindle assembly checkpoint / establishment of centrosome localization / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / nuclear pore nuclear basket / negative regulation of ubiquitin protein ligase activity / mitotic spindle assembly checkpoint signaling / mitotic sister chromatid segregation / establishment of mitotic spindle orientation / negative regulation of mitotic cell cycle / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / APC-Cdc20 mediated degradation of Nek2A / RHO GTPases Activate Formins / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / negative regulation of protein catabolic process / 紡錘体 / 動原体 / 紡錘体 / Separation of Sister Chromatids / 細胞分裂 / negative regulation of apoptotic process / perinuclear region of cytoplasm / protein homodimerization activity / 核質 / identical protein binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Luo, X. / Fang, G. / Coldiron, M. / Lin, Y. / Yu, H. | ||||||
引用 | ジャーナル: Nat.Struct.Biol. / 年: 2000 タイトル: Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. 著者: Luo, X. / Fang, G. / Coldiron, M. / Lin, Y. / Yu, H. / Kirschner, M.W. / Wagner, G. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1duj.cif.gz | 71.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1duj.ent.gz | 54.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1duj.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/du/1duj ftp://data.pdbj.org/pub/pdb/validation_reports/du/1duj | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 21430.369 Da / 分子数: 1 断片: FULL PROTEIN WITHOUT BOTH N- AND C-TERMINAL 10 RESIDUES 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PQE-30 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q13257 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-試料調製
詳細 |
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試料状態 |
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結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: The structures are based on a total of 3111 restraints, 2807 are NOE-derived distance constraints, 214 dihedral angle restraints,90 distance restraints from hydrogen bonds. | ||||||||||||
NMRアンサンブル | 登録したコンフォーマーの数: 1 |