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- PDB-1dc9: PROPERTIES AND CRYSTAL STRUCTURE OF A BETA-BARREL FOLDING MUTANT,... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1dc9 | ||||||
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Title | PROPERTIES AND CRYSTAL STRUCTURE OF A BETA-BARREL FOLDING MUTANT, V60N INTESTINAL FATTY ACID BINDING PROTEIN (IFABP) | ||||||
![]() | INTESTINAL FATTY ACID BINDING PROTEIN | ||||||
![]() | LIPID BINDING PROTEIN / ![]() ![]() | ||||||
Function / homology | ![]() Triglyceride catabolism / intestinal lipid absorption / apical cortex / intestinal absorption / long-chain fatty acid transmembrane transporter activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Ropson, I.J. / Yowler, B.C. / Dalessio, P.M. / Banaszak, L. / Thompson, J. | ||||||
![]() | ![]() Title: Properties and crystal structure of a beta-barrel folding mutant. Authors: Ropson, I.J. / Yowler, B.C. / Dalessio, P.M. / Banaszak, L. / Thompson, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.8 KB | Display | ![]() |
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PDB format | ![]() | 26.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15029.987 Da / Num. of mol.: 1 / Mutation: V60N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39 % | ||||||||||||||||||||||||
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Crystal grow![]() | Method: vapor diffusion, hanging drop / pH: 7.3 Details: 36-38% PEG 4000, 0.1 M PIPES, 1:1 RATIO WELL AND 4.6 MG/ML PROTEIN IN WATER, pH 7.3, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: Mar 5, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.1→20 Å / Num. all: 6804 / Num. obs: 6804 / % possible obs: 91 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Biso Wilson estimate: 5.7 Å2 / Rmerge(I) obs: 0.034 / Net I/σ(I): 23.9 |
Reflection shell | Resolution: 2.09→2.22 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.145 / % possible all: 38.2 |
Reflection | *PLUS Num. measured all: 19796 / Rmerge(I) obs: 0.047 |
Reflection shell | *PLUS % possible obs: 38.2 % / Num. unique obs: 447 / Num. measured obs: 779 / Rmerge(I) obs: 0.151 / Mean I/σ(I) obs: 1.4 |
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Processing
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Refinement | Resolution: 2.1→20 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 158121.65 / Data cutoff high rms absF: 158121.65 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Displacement parameters | Biso mean: 18.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.049 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.3 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 8 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 18.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.362 / % reflection Rfree: 10.6 % / Rfactor Rwork: 0.251 |