+Open data
-Basic information
Entry | Database: PDB / ID: 1d27 | ||||||||||||||||||
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Title | HIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNA | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / B-DNA / DOUBLE HELIX / MODIFIED / MISMATCHED | Function / homology | DNA / DNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / Resolution: 2 Å | Authors | Leonard, G.A. / Thomson, J. / Watson, W.P. / Brown, T. | Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1990 | Title: High-resolution structure of a mutagenic lesion in DNA. Authors: Leonard, G.A. / Thomson, J. / Watson, W.P. / Brown, T. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1d27.cif.gz | 25 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1d27.ent.gz | 15.9 KB | Display | PDB format |
PDBx/mmJSON format | 1d27.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d2/1d27 ftp://data.pdbj.org/pub/pdb/validation_reports/d2/1d27 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3692.430 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.82 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 6.3 / Details: pH 6.30, VAPOR DIFFUSION, temperature 277.00K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 277 K / pH: 6.3 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Highest resolution: 2 Å / Num. obs: 4557 | ||||||||||||
Reflection | *PLUS Highest resolution: 2 Å / % possible obs: 91 % / Rmerge(I) obs: 0.03 |
-Processing
Software | Name: NUCLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2→7 Å / σ(F): 3 /
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 2→7 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 7 Å / σ(F): 3 / Rfactor obs: 0.185 / Num. reflection obs: 3118 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |