+Open data
-Basic information
Entry | Database: PDB / ID: 1ck2 | ||||||
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Title | YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30 | ||||||
Components | 60S RIBOSOMAL PROTEIN L30 | ||||||
Keywords | RIBOSOME / RIBOSOMAL PROTEIN / AUTO-REGULATION OF PRE-MRNA SPLICING AND MRNA TRANSLATION | ||||||
Function / homology | Function and homology information pre-mRNA 5'-splice site binding / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / rRNA processing / cytoplasmic translation ...pre-mRNA 5'-splice site binding / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / rRNA processing / cytoplasmic translation / cytosolic large ribosomal subunit / structural constituent of ribosome / RNA binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING, MOLECULAR DYNAMIC | ||||||
Authors | Mao, H. / Willamson, J.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1999 Title: Local folding coupled to RNA binding in the yeast ribosomal protein L30 Authors: Mao, H. / Williamson, J.R. #1: Journal: J.Biol.Chem. / Year: 1987 Title: The Yeast Ribosomal Protein L32 and its Gene Authors: Dabeva, M.D. / Warner, J.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ck2.cif.gz | 43.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ck2.ent.gz | 30.6 KB | Display | PDB format |
PDBx/mmJSON format | 1ck2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ck/1ck2 ftp://data.pdbj.org/pub/pdb/validation_reports/ck/1ck2 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11299.168 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: This entry, 1CK2, is the replacement of 1CN6 cited in the primary reference Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Description: RECOMBINANT EXPRESSION AS A C-TERMINAL MALTOSE-BINDING PROTEIN FUSION IN ESCHERICHIA COLI STRAIN JM109 HOSTING PLASMID PMALC-30 Cellular location: CYTOPLASM / Gene: RPL30 / Organelle: RIBOSOME, 60S SUBUNIT / Plasmid: PMALC-L30 / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P14120 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: MEAN STRUCTURE. THIS STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED L30 PROTEIN. |
-Sample preparation
Details | Contents: 10% WATER/90% D2O |
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Sample conditions | Ionic strength: 300 mM / pH: 6.5 / Pressure: 1 ATOM / Temperature: 288 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian VARIAN INOVA / Manufacturer: Varian / Model: VARIAN INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING, MOLECULAR DYNAMIC / Software ordinal: 1 Details: DISTANCE INTRARESIDUE NOES 720 INTERRESIDUE NOES 698 SEQUENTIAL (|I-J|=1) 291 SHORT-RANGE (|I-J|<=4) 149, LONG-RANGE (|I-J|>4) 258, HYDROGEN BONDS 62. TOTAL 1418, TORSION BACKBONE PHI 80, SIDECHAIN KAI1 59, TOTAL 139 | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TOTAL ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 1 |