+Open data
-Basic information
Entry | Database: PDB / ID: 1bm9 | ||||||
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Title | REPLICATION TERMINATOR PROTEIN FROM BACILLUS SUBTILIS | ||||||
Components | REPLICATION TERMINATOR PROTEINReplication terminator Tus family | ||||||
Keywords | DNA BINDING PROTEIN / DNA-BINDING PROTEIN / CONTRAHELICASE | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2 Å | ||||||
Authors | Bussiere, D.E. / Bastia, D. / White, S. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1995 Title: Crystal structure of the replication terminator protein from B. subtilis at 2.6 A. Authors: Bussiere, D.E. / Bastia, D. / White, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bm9.cif.gz | 73.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bm9.ent.gz | 56.9 KB | Display | PDB format |
PDBx/mmJSON format | 1bm9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bm/1bm9 ftp://data.pdbj.org/pub/pdb/validation_reports/bm/1bm9 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.1813, 0.1268, 0.9752), Vector: |
-Components
#1: Protein | Mass: 14547.163 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Cell line: BL21 / Gene: RTP / Plasmid: PET-13A / Species (production host): Escherichia coli / Gene (production host): RTP / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P68732, UniProt: P0CI76*PLUS #2: Water | ChemComp-HOH / | Compound details | THIS DNA-BINDING PROTEIN IS A DIMER OF TWO WINGED HELIX MOTIFS JOINED BY AN ANTIPARALLEL COILED- ...THIS DNA-BINDING PROTEIN IS A DIMER OF TWO WINGED HELIX MOTIFS JOINED BY AN ANTIPARALL | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 50 % | |||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | |||||||||||||||
Crystal grow | *PLUS Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jun 1, 1994 / Details: CRYSTAL/COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→10 Å / Num. obs: 16299 / % possible obs: 84.9 % / Observed criterion σ(I): 2 / Redundancy: 2 % / Biso Wilson estimate: 22.4 Å2 / Rmerge(I) obs: 0.06 / Rsym value: 0.1 / Net I/σ(I): 7 |
Reflection shell | Resolution: 2→2.2 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.07 / Mean I/σ(I) obs: 2 / Rsym value: 0.2 / % possible all: 70 |
Reflection shell | *PLUS % possible obs: 70 % |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2→10 Å / Rfactor Rfree error: 0.008 / Data cutoff high rms absF: 501847.89 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 44.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.12 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.3 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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