+Open data
-Basic information
Entry | Database: PDB / ID: 1ahq | ||||||
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Title | RECOMBINANT ACTOPHORIN | ||||||
Components | ACTOPHORIN | ||||||
Keywords | ACTIN BINDING PROTEIN / ACTOPHORIN | ||||||
Function / homology | Function and homology information actin filament depolymerization / actin cytoskeleton / actin binding / cytoplasm Similarity search - Function | ||||||
Biological species | Acanthamoeba castellanii (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / MIR X-RAY CRYSTAL / Resolution: 2.3 Å | ||||||
Authors | Leonard, S.A. / Gittis, A.G. / Petrella, E.C. / Pollard, T.D. / Lattman, E.E. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Crystal structure of the actin-binding protein actophorin from Acanthamoeba. Authors: Leonard, S.A. / Gittis, A.G. / Petrella, E.C. / Pollard, T.D. / Lattman, E.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ahq.cif.gz | 43.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ahq.ent.gz | 31 KB | Display | PDB format |
PDBx/mmJSON format | 1ahq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ah/1ahq ftp://data.pdbj.org/pub/pdb/validation_reports/ah/1ahq | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15444.394 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acanthamoeba castellanii (eukaryote) / Cell line: BL21 / Plasmid: PACT / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P37167 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 4 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 40 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: 20-25% PEG 8000, 100MM HEPES (PH7.5), 10% ISOPROPANOL, 1MM DTT | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: May 12, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.06→10 Å / Num. obs: 7897 / % possible obs: 95 % / Observed criterion σ(I): 1 / Redundancy: 3.3 % / Rmerge(I) obs: 0.19 / Rsym value: 0.6 |
Reflection shell | Resolution: 2.06→2.25 Å / Mean I/σ(I) obs: 5.3 / % possible all: 92.5 |
Reflection | *PLUS Num. measured all: 26016 / Rmerge(I) obs: 0.068 |
Reflection shell | *PLUS % possible obs: 92.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MIR X-RAY CRYSTAL / Resolution: 2.3→6 Å / σ(F): 6
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Refinement step | Cycle: LAST / Resolution: 2.3→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.34 Å / Total num. of bins used: 20
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.38 |