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Open data
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Basic information
Entry | Database: PDB / ID: 1a0r | ||||||
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Title | HETEROTRIMERIC COMPLEX OF PHOSDUCIN/TRANSDUCIN BETA-GAMMA | ||||||
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![]() | COMPLEX (TRANSDUCER/TRANSDUCTION) / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() Olfactory Signaling Pathway / Sensory perception of sweet, bitter, and umami (glutamate) taste / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Inactivation, recovery and regulation of the phototransduction cascade / Activation of the phototransduction cascade / response to stimulus / Activation of G protein gated Potassium channels / G-protein activation / G beta:gamma signalling through PI3Kgamma / Prostacyclin signalling through prostacyclin receptor ...Olfactory Signaling Pathway / Sensory perception of sweet, bitter, and umami (glutamate) taste / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Inactivation, recovery and regulation of the phototransduction cascade / Activation of the phototransduction cascade / response to stimulus / Activation of G protein gated Potassium channels / G-protein activation / G beta:gamma signalling through PI3Kgamma / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through PLC beta / ADP signalling through P2Y purinoceptor 1 / Thromboxane signalling through TP receptor / Presynaptic function of Kainate receptors / G beta:gamma signalling through CDC42 / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Glucagon-type ligand receptors / Adrenaline,noradrenaline inhibits insulin secretion / G alpha (12/13) signalling events / G beta:gamma signalling through BTK / ADP signalling through P2Y purinoceptor 12 / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Thrombin signalling through proteinase activated receptors (PARs) / Ca2+ pathway / G alpha (z) signalling events / Extra-nuclear estrogen signaling / G alpha (s) signalling events / G alpha (q) signalling events / G alpha (i) signalling events / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Vasopressin regulates renal water homeostasis via Aquaporins / photoreceptor outer segment / ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Loew, A. / Ho, Y.-K. / Blundell, T.L. / Bax, B. | ||||||
![]() | ![]() Title: Phosducin induces a structural change in transducin beta gamma. Authors: Loew, A. / Ho, Y.K. / Blundell, T. / Bax, B. #1: ![]() Title: Crystal Structure at 2.4 Angstroms Resolution of the Complex of Transducin Betagamma and its Regulator, Phosducin Authors: Gaudet, R. / Bohm, A. / Sigler, P.B. #2: ![]() Title: The Structure of the G Protein Heterotrimer Gi Alpha 1 Beta 1 Gamma 2 Authors: Wall, M.A. / Coleman, D.E. / Lee, E. / Iniguez-Lluhi, J.A. / Posner, B.A. / Gilman, A.G. / Sprang, S.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 128.1 KB | Display | ![]() |
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PDB format | ![]() | 98.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1gp2S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37325.797 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PURIFIED FROM BOVINE ROD OUTER SEGMENT / Source: (natural) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 7666.770 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PURIFIED FROM BOVINE ROD OUTER SEGMENT / Source: (natural) ![]() ![]() ![]() ![]() |
#3: Protein | ![]() Mass: 28268.758 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: PURIFIED FROM BOVINE ROD OUTER SEGMENT IN COMPLEX WITH TRANSDUCIN BETA-GAMMA SUBUNIT Source: (natural) ![]() ![]() ![]() ![]() |
#4: Chemical | ChemComp-FAR / ![]() |
#5: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 277 K / Method: microbatch / pH: 6.8 Details: THE PROTEIN COMPLEX (10 MG/ML SOLUTION) WAS CRYSTALLIZED FROM 400 MM SODIUM CACODYLATE (PH 6.8), 1 MM ZINC CHLORIDE, 25 % ETHYLENE GLYCOL, 12 % PEG 8000, BY MICROBATCH CRYSTALLIZATION AT 4 ...Details: THE PROTEIN COMPLEX (10 MG/ML SOLUTION) WAS CRYSTALLIZED FROM 400 MM SODIUM CACODYLATE (PH 6.8), 1 MM ZINC CHLORIDE, 25 % ETHYLENE GLYCOL, 12 % PEG 8000, BY MICROBATCH CRYSTALLIZATION AT 4 DEGREES C., microbatch, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: batch method | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD / Date: Apr 1, 1996 / Details: MIRRORS |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.8→20 Å / Num. obs: 15784 / % possible obs: 93.2 % / Observed criterion σ(I): 0 / Redundancy: 3.09 % / Biso Wilson estimate: 31.6 Å2 / Rsym value: 0.08 |
Reflection shell | Resolution: 2.8→2.9 Å / Rsym value: 0.315 / % possible all: 97.8 |
Reflection | *PLUS Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS % possible obs: 97.8 % / Rmerge(I) obs: 0.315 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: BETA-GAMMA CHAIN OF 1GP2 Resolution: 2.8→20 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Details: BULK SOLVENT MODEL USED
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Displacement parameters | Biso mean: 31.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.93 Å / Rfactor Rfree error: 0.047 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.222 / Rfactor Rfree![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.296 |