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- EMDB-42987: Structure of the Human Respirovirus 3 Fusion Protein Bound to Cam... -
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Open data
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Basic information
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Title | Structure of the Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06 | |||||||||
![]() | Final refined volume. DeepEMhancer sharpened. Used to build coordinates. | |||||||||
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![]() | viral fusion protein / camelid nanobodies / viral glycoprotein / ![]() ![]() ![]() | |||||||||
Function / homology | Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0 / fusion of virus membrane with host plasma membrane / ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Johnson NJ / Ramamohan AR / McLellan JS | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structural basis for potent neutralization of human respirovirus type 3 by single-domain camelid antibodies Authors: Johnson NV / van Scherpenzeel RC / Bakkers MJG / Ramamohan AR / van Overveld D / Le L / Langedijk JPM / Kolkman JA / McLellan JS | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 108.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18 KB 18 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.6 KB | Display | ![]() |
Images | ![]() | 65.8 KB | ||
Filedesc metadata | ![]() | 5.9 KB | ||
Others | ![]() ![]() ![]() | 118 MB 116.1 MB 116.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8v62MC ![]() 8v5kC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Final refined volume. DeepEMhancer sharpened. Used to build coordinates. | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.94 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Final refined volume. Sharpened map, not DeepEMhancer sharpened.
File | emd_42987_additional_1.map | ||||||||||||
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Annotation | Final refined volume. Sharpened map, not DeepEMhancer sharpened. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map A
File | emd_42987_half_map_1.map | ||||||||||||
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Annotation | half map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map B
File | emd_42987_half_map_2.map | ||||||||||||
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Annotation | half map B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Prefusion Human Respirovirus 3 Fusion Protein Bound to Camelid Na...
Entire | Name: Prefusion Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06 |
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Components |
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-Supramolecule #1: Prefusion Human Respirovirus 3 Fusion Protein Bound to Camelid Na...
Supramolecule | Name: Prefusion Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06 type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 277.5 KDa |
-Macromolecule #1: Fusion glycoprotein F0
Macromolecule | Name: Fusion glycoprotein F0 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 57.336508 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MPISILLIIT TMIMASHCQI DITKLQHVGV LVNSPKGMKI SQNFETRYLI LSLIPKIEDS NSCGDQQIKQ YKRLLDRLII PLYDGLRLQ KDVIVTNQES NENTDPRTER FFGGVIGTIA LGVATSAQIT AAVALVEAKQ ARSDIEKLKE AIRDTNKAVQ S VQSSPGNL ...String: MPISILLIIT TMIMASHCQI DITKLQHVGV LVNSPKGMKI SQNFETRYLI LSLIPKIEDS NSCGDQQIKQ YKRLLDRLII PLYDGLRLQ KDVIVTNQES NENTDPRTER FFGGVIGTIA LGVATSAQIT AAVALVEAKQ ARSDIEKLKE AIRDTNKAVQ S VQSSPGNL IVAIKSVQDY VNKEIVPCIA RLGCEACGLL LGLALDQHYS ELTNIFGDNI GSLQEKGIKL QGIASLYRTN IT EIFTTST VDKYDIYDLL FTESIKVRVI DVDLNDYSIT LQVRLPLLTR LLNTQIYKVD SISYNIQNRE WYIPLPSHIM TKG AFLGGA DVKECIEAFS SYICPSDPGF VLNHEMESCL SGNISQCPRT TVTSDIVPRY AFVNGGVVAN CITTTCTCNG IGNR INQPP DQGVKIITHK ECNTIGINGM LFNTNKEGTL AFYTPDDITL NNSVALNPID ISIELNKAKS DLEESKEWIR RSNQK LDSI EDKIEEILSK IYHIENEIAR IKKLIGEAEP EA UniProtKB: Fusion glycoprotein F0 |
-Macromolecule #2: Camelid nanobody 1D10
Macromolecule | Name: Camelid nanobody 1D10 / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 12.436922 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: EVQLVESGGG LVQTGDSLRL SCAASGSIFG ENAMAWFRQA PGKQRELVAR VSTGGTLFYA DFAKVRFTIS RDTAKQTVYL QMSSLRPED TAVYYCAVAV GTRNYWGQGT QVTVSS |
-Macromolecule #3: Camelid nanobody 4C06
Macromolecule | Name: Camelid nanobody 4C06 / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 12.487816 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: EVQLVESGGG LVQPGGSLRL SCSASGSLST IKALGWYRRA PGRERELVAS ITSAGETNYA DSAKGRFTVS TDNAKNTVDL RMNSLKPED TAVYYCYAES FVLNIYWGQG TQVTVSSG |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS GLACIOS |
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Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |