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Yorodumi- EMDB-35909: Cryo-EM structure of Mycobacterium tuberculosis ATP synthase in c... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35909 | ||||||||||||
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Title | Cryo-EM structure of Mycobacterium tuberculosis ATP synthase in complex with bedaquiline(BDQ) | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | ATP synthase / Mycobacterium tuberculosis / cryo-EM / MEMBRANE PROTEIN | ||||||||||||
Function / homology | Function and homology information photosynthetic electron transport in photosystem I / proton motive force-driven plasma membrane ATP synthesis / photosynthetic electron transport in photosystem II / proton-transporting ATP synthase complex, coupling factor F(o) / proton motive force-driven ATP synthesis / proton-transporting ATP synthase complex, catalytic core F(1) / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / peptidoglycan-based cell wall ...photosynthetic electron transport in photosystem I / proton motive force-driven plasma membrane ATP synthesis / photosynthetic electron transport in photosystem II / proton-transporting ATP synthase complex, coupling factor F(o) / proton motive force-driven ATP synthesis / proton-transporting ATP synthase complex, catalytic core F(1) / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / peptidoglycan-based cell wall / ADP binding / hydrolase activity / lipid binding / ATP hydrolysis activity / extracellular region / ATP binding / plasma membrane / cytosol Similarity search - Function | ||||||||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.58 Å | ||||||||||||
Authors | Zhang Y / Lai Y / Liu F / Rao Z / Gong H | ||||||||||||
Funding support | China, 3 items
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Citation | Journal: To Be Published Title: Structure of Mycobacterium tuberculosis ATP synthase Authors: Zhang Y / Lai Y / Liu F / Rao Z / Gong H | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35909.map.gz | 471.5 MB | EMDB map data format | |
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Header (meta data) | emd-35909-v30.xml emd-35909.xml | 21.1 KB 21.1 KB | Display Display | EMDB header |
Images | emd_35909.png | 42.2 KB | ||
Filedesc metadata | emd-35909.cif.gz | 7.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35909 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35909 | HTTPS FTP |
-Related structure data
Related structure data | 8j0sMC 8j0tC 8j57C 8j58C 8jr0C 8jr1C 8khfC 8ki3C C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_35909.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.73 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
+Entire : Mycobacterium tuberculosis ATP synthase in complex with bedaquili...
+Supramolecule #1: Mycobacterium tuberculosis ATP synthase in complex with bedaquili...
+Macromolecule #1: ATP synthase subunit a
+Macromolecule #2: ATP synthase subunit alpha
+Macromolecule #3: ATP synthase subunit beta
+Macromolecule #4: ATP synthase gamma chain
+Macromolecule #5: ATP synthase epsilon chain
+Macromolecule #6: ATP synthase subunit c
+Macromolecule #7: ATP synthase subunit b
+Macromolecule #8: Multifunctional fusion protein
+Macromolecule #9: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #10: MAGNESIUM ION
+Macromolecule #11: ADENOSINE-5'-DIPHOSPHATE
+Macromolecule #12: Bedaquiline
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.2 µm |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER / Details: AlphaFold |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.58 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 96592 |