+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-34496 | ||||||||||||
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Title | The core region map of avian influenza A polymerase. | ||||||||||||
Map data | The core region map of avian influenza A polymerase. | ||||||||||||
Sample |
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Keywords | Polymerase / VIRAL PROTEIN | ||||||||||||
Biological species | Influenza A virus | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | ||||||||||||
Authors | Li H / Wu Y / Liang H / Liu Y | ||||||||||||
Funding support | China, 3 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2023 Title: An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles. Authors: Huanhuan Li / Yixi Wu / Minke Li / Lu Guo / Yaqi Gao / Quan Wang / Jihua Zhang / Zhaohua Lai / Xing Zhang / Lixin Zhu / Ping Lan / Zihe Rao / Yingfang Liu / Huanhuan Liang / Abstract: Influenza polymerase (FluPol) transcribes viral mRNA at the beginning of the viral life cycle and initiates genome replication after viral protein synthesis. However, it remains poorly understood how ...Influenza polymerase (FluPol) transcribes viral mRNA at the beginning of the viral life cycle and initiates genome replication after viral protein synthesis. However, it remains poorly understood how FluPol switches between its transcription and replication states, especially given that the structural bases of these two functions are fundamentally different. Here we propose a mechanism by which FluPol achieves functional switching between these two states through a previously unstudied conformation, termed an 'intermediate state'. Using cryo-electron microscopy, we obtained a structure of the intermediate state of H5N1 FluPol at 3.7 Å, which is characterized by a blocked cap-binding domain and a contracted core region. Structural analysis results suggest that the intermediate state may allow FluPol to transition smoothly into either the transcription or replication state. Furthermore, we show that the formation of the intermediate state is required for both the transcription and replication activities of FluPol, leading us to conclude that the transcription and replication cycles of FluPol are regulated via this intermediate state. | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_34496.map.gz | 4.2 MB | EMDB map data format | |
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Header (meta data) | emd-34496-v30.xml emd-34496.xml | 13.1 KB 13.1 KB | Display Display | EMDB header |
Images | emd_34496.png | 33.6 KB | ||
Others | emd_34496_half_map_1.map.gz emd_34496_half_map_2.map.gz | 13.3 MB 13.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-34496 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34496 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_34496.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | The core region map of avian influenza A polymerase. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: The half1 map of core region of avian influenza A polymerase.
File | emd_34496_half_map_1.map | ||||||||||||
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Annotation | The half1 map of core region of avian influenza A polymerase. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: The half2 map of core region of avian influenza A polymerase.
File | emd_34496_half_map_2.map | ||||||||||||
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Annotation | The half2 map of core region of avian influenza A polymerase. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Influenza A virus RdRp complex
Entire | Name: Influenza A virus RdRp complex |
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Components |
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-Supramolecule #1: Influenza A virus RdRp complex
Supramolecule | Name: Influenza A virus RdRp complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Influenza A virus |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 8.3 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 779360 |
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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