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Yorodumi- EMDB-19444: Alpha-synuclein amyloid fibrils with WT DNAJB1, HSC70, HSP110 and ATP -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-19444 | ||||||||||||
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Title | Alpha-synuclein amyloid fibrils with WT DNAJB1, HSC70, HSP110 and ATP | ||||||||||||
Map data | Tomogram of alpha-synuclein amyloid fibrils incibated with WT DNAJB1, Hsc70, Hsp110 and ATP | ||||||||||||
Sample |
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Keywords | Chaperones / amyloid fibril / disaggregation / PROTEIN FIBRIL | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | electron tomography / cryo EM | ||||||||||||
Authors | Saibil HR / Monistrol J | ||||||||||||
Funding support | United Kingdom, 3 items
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Citation | Journal: Biorxiv / Year: 2024 Title: Stepwise recruitment of Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibre disassembly Authors: Monistrol J / Beton JG / Johnston EC / Saibil HR | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_19444.map.gz | 6.3 GB | EMDB map data format | |
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Header (meta data) | emd-19444-v30.xml emd-19444.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
Images | emd_19444.png | 160.6 KB | ||
Filedesc metadata | emd-19444.cif.gz | 4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-19444 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-19444 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_19444.map.gz / Format: CCP4 / Size: 6.8 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Tomogram of alpha-synuclein amyloid fibrils incibated with WT DNAJB1, Hsc70, Hsp110 and ATP | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.8 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Alpha-synuclein amyloid fibrils incubated with WT DNAJB1, HSC70, ...
Entire | Name: Alpha-synuclein amyloid fibrils incubated with WT DNAJB1, HSC70, HSP110 and ATP |
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Components |
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-Supramolecule #1: Alpha-synuclein amyloid fibrils incubated with WT DNAJB1, HSC70, ...
Supramolecule | Name: Alpha-synuclein amyloid fibrils incubated with WT DNAJB1, HSC70, HSP110 and ATP type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.5 Component:
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Grid | Model: C-flat-1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber temperature: 277.15 K / Instrument: LEICA EM GP | ||||||||||||||||||||||||
Sectioning | Other: NO SECTIONING | ||||||||||||||||||||||||
Fiducial marker | Manufacturer: EMS / Diameter: 10 nm |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 6.5 µm / Nominal defocus min: 2.0 µm |
Specialist optics | Energy filter - Name: TFS Selectris |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 118.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Software - Name: RELION / Number images used: 121 |
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