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- EMDB-17085: Structure of Human Solute Carrier 26 family member A6 (SLC26A6) a... -
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Open data
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Basic information
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Title | Structure of Human Solute Carrier 26 family member A6 (SLC26A6) anion transporter in an inward-facing state | |||||||||
![]() | Final cryo-EM density map of human SLC26A6 | |||||||||
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Function / homology | ![]() sulfate:bicarbonate antiporter activity / formate transport / mannitol transmembrane transport / oxalate transport / formate transmembrane transporter activity / Multifunctional anion exchangers / oxalic acid secretion / sulfate transport / protein kinase C signaling / transepithelial chloride transport ...sulfate:bicarbonate antiporter activity / formate transport / mannitol transmembrane transport / oxalate transport / formate transmembrane transporter activity / Multifunctional anion exchangers / oxalic acid secretion / sulfate transport / protein kinase C signaling / transepithelial chloride transport / epithelial fluid transport / sulfate transmembrane transporter activity / secondary active sulfate transmembrane transporter activity / oxalate transmembrane transporter activity / intracellular pH elevation / cellular response to fructose stimulus / chloride:bicarbonate antiporter activity / solute:inorganic anion antiporter activity / vesicle membrane / transepithelial transport / bicarbonate transport / bicarbonate transmembrane transporter activity / intestinal absorption / chloride transport / chloride transmembrane transporter activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Tippett DN / Breen C / Butler SJ / Sawicka M / Dutzler R | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange. Authors: David N Tippett / Colum Breen / Stephen J Butler / Marta Sawicka / Raimund Dutzler / ![]() ![]() Abstract: Members of the SLC26 family constitute a conserved class of anion transport proteins, which encompasses uncoupled transporters with channel-like properties, coupled exchangers and motor proteins. ...Members of the SLC26 family constitute a conserved class of anion transport proteins, which encompasses uncoupled transporters with channel-like properties, coupled exchangers and motor proteins. Among the 10 functional paralogs in humans, several participate in the secretion of bicarbonate in exchange with chloride and thus play an important role in maintaining pH homeostasis. Previously, we have elucidated the structure of murine SLC26A9 and defined its function as an uncoupled chloride transporter (Walter et al., 2019). Here we have determined the structure of the closely related human transporter SLC26A6 and characterized it as a coupled exchanger of chloride with bicarbonate and presumably also oxalate. The structure defines an inward-facing conformation of the protein that generally resembles known structures of SLC26A9. The altered anion selectivity between both paralogs is a consequence of a remodeled ion binding site located in the center of a mobile unit of the membrane-inserted domain, which also accounts for differences in the coupling mechanism. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 277.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.7 KB 16.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.3 KB | Display | ![]() |
Images | ![]() | 122.3 KB | ||
Masks | ![]() | 307.5 MB | ![]() | |
Others | ![]() ![]() | 284.8 MB 284.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8opqMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Final cryo-EM density map of human SLC26A6 | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.651 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half-map 2 of human SLC26A6
File | emd_17085_half_map_1.map | ||||||||||||
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Annotation | Half-map 2 of human SLC26A6 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map 1 of human SLC26A6
File | emd_17085_half_map_2.map | ||||||||||||
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Annotation | Half-map 1 of human SLC26A6 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Homodimer of the human SLC26A6 anion transporter
Entire | Name: Homodimer of the human SLC26A6 anion transporter |
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Components |
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-Supramolecule #1: Homodimer of the human SLC26A6 anion transporter
Supramolecule | Name: Homodimer of the human SLC26A6 anion transporter / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Solute carrier family 26 member 6
Macromolecule | Name: Solute carrier family 26 member 6 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 83.812328 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MSGLADASGP RDTQALLSAT QAMDLRRRDY HMERPLLNQE HLEELGRWGS APRTHQWRTW LQCSRARAYA LLLQHLPVLV WLPRYPVRD WLLGDLLSGL SVAIMQLPQG LAYALLAGLP PVFGLYSSFY PVFIYFLFGT SRHISVGTFA VMSVMVGSVT E SLAPQALN ...String: MSGLADASGP RDTQALLSAT QAMDLRRRDY HMERPLLNQE HLEELGRWGS APRTHQWRTW LQCSRARAYA LLLQHLPVLV WLPRYPVRD WLLGDLLSGL SVAIMQLPQG LAYALLAGLP PVFGLYSSFY PVFIYFLFGT SRHISVGTFA VMSVMVGSVT E SLAPQALN DSMINETARD AARVQVASTL SVLVGLFQVG LGLIHFGFVV TYLSEPLVRG YTTAAAVQVF VSQLKYVFGL HL SSHSGPL SLIYTVLEVC WKLPQSKVGT VVTAAVAGVV LVVVKLLNDK LQQQLPMPIP GELLTLIGAT GISYGMGLKH RFE VDVVGN IPAGLVPPVA PNTQLFSKLV GSAFTIAVVG FAIAISLGKI FALRHGYRVD SNQELVALGL SNLIGGIFQC FPVS CSMSR SLVQESTGGN SQVAGAISSL FILLIIVKLG ELFHDLPKAV LAAIIIVNLK GMLRQLSDMR SLWKANRADL LIWLV TFTA TILLNLDLGL VVAVIFSLLL VVVRTQMPHY SVLGQVPDTD IYRDVAEYSE AKEVRGVKVF RSSATVYFAN AEFYSD ALK QRCGVDVDFL ISQKKKLLKK QEQLKLKQLQ KEEKLRKQAA SPKGASVSIN VNTSLEDMRS NNVEDCKMMV SSGDKME DA TANGQEDSKA PDGSTLKALG LPQPDFHSLI LDLGALSFVD TVCLKSLKNI FHDFREIEVE VYMAACHSPV VSQLEAGH F FDASITKKHL FASVHDAVTF ALQHPRPVPD SPVSVTRLAL EVLFQ |
-Macromolecule #2: CHLORIDE ION
Macromolecule | Name: CHLORIDE ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: CL |
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Molecular weight | Theoretical: 35.453 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 2 mg/mL | ||||||||||||
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Buffer | pH: 7.4 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | ||||||||||||
Vitrification | Cryogen name: ETHANE-PROPANE | ||||||||||||
Details | Full-lengthed Human SLC26A6 expressed in HEK293S GNTi- cells |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 2 / Number real images: 11962 / Average electron dose: 67.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |