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- EMDB-16389: Cryo-EM structure of photosystem II C2S2 supercomplex from Norway... -

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Entry
Database: EMDB / ID: EMD-16389
TitleCryo-EM structure of photosystem II C2S2 supercomplex from Norway spruce (Picea babies) at 2.8 Angstrom resolution
Map data
Sample
  • Complex: The C2S2-type PSII-LHCII supercomplex
    • Complex: Photosystem II core complex (C2)
      • Protein or peptide: x 18 types
    • Complex: Photosystem II light-harvesting complex
      • Protein or peptide: x 3 types
  • Ligand: x 22 types
KeywordsMembrane protein complex / Photosynthesis / Photosystem / Gymnosperm / MEMBRANE PROTEIN
Function / homology
Function and homology information


photosynthesis, light harvesting / photosystem II assembly / photosystem II stabilization / oxygen evolving activity / photosystem II oxygen evolving complex / photosystem II / photosystem II reaction center / photosynthetic electron transport chain / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I ...photosynthesis, light harvesting / photosystem II assembly / photosystem II stabilization / oxygen evolving activity / photosystem II oxygen evolving complex / photosystem II / photosystem II reaction center / photosynthetic electron transport chain / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / response to herbicide / photosystem II / photosynthetic electron transport in photosystem II / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding
Similarity search - Function
Photosystem II 5kDa protein, chloroplastic / Photosystem II PsbW, class 2 / Photosystem II reaction centre W protein (PsbW) / Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbO, manganese-stabilising / Manganese-stabilising protein / photosystem II polypeptide / Photosystem II reaction centre M protein (PsbM) / Photosystem II PsbM superfamily / Photosystem II PsbZ, reaction centre ...Photosystem II 5kDa protein, chloroplastic / Photosystem II PsbW, class 2 / Photosystem II reaction centre W protein (PsbW) / Photosystem II reaction centre protein Ycf12 / Photosystem II complex subunit Ycf12 / Photosystem II PsbO, manganese-stabilising / Manganese-stabilising protein / photosystem II polypeptide / Photosystem II reaction centre M protein (PsbM) / Photosystem II PsbM superfamily / Photosystem II PsbZ, reaction centre / Photosystem II PsbZ superfamily / YCF9 / Photosystem II PsbM / Photosystem II PsbX / Photosystem II reaction centre X protein (PsbX) / Photosystem II PsbT / Photosystem II PsbL / Photosystem II PsbL superfamily / Photosystem II PsbT superfamily / Photosystem II CP43 reaction centre protein superfamily / Photosystem II reaction centre T protein / PsbL protein / Photosystem II PsbK / Photosystem II CP43 reaction centre protein / Photosystem II PsbK superfamily / Photosystem II 4 kDa reaction centre component / Photosystem II CP47 reaction centre protein / Photosystem II PsbI / Photosystem II PsbI superfamily / Photosystem II reaction centre I protein (PSII 4.8 kDa protein) / Photosystem II reaction centre protein H / Photosystem II protein D1 / Photosystem II D2 protein / Photosystem II cytochrome b559, conserved site / Photosystem II cytochrome b559, alpha subunit / Photosystem II cytochrome b559, beta subunit / Photosystem II cytochrome b559, N-terminal / Photosystem II cytochrome b559, alpha subunit, lumenal region / Photosystem II reaction centre protein H superfamily / Photosystem II cytochrome b559, alpha subunit superfamily / Cytochrome b559, alpha (gene psbE) and beta (gene psbF)subunits / Lumenal portion of Cytochrome b559, alpha (gene psbE) subunit / Photosystem II 10 kDa phosphoprotein / Cytochrome b559 subunits heme-binding site signature. / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Chlorophyll A-B binding protein, plant and chromista / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Outer membrane protein/outer membrane enzyme PagP, beta-barrel / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature.
Similarity search - Domain/homology
Photosystem II reaction center protein M / Photosystem II 5 kDa protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / PSII 6.1 kDa protein / Uncharacterized protein / Uncharacterized protein / Photosystem II protein D1 / Chlorophyll a-b binding protein, chloroplastic / Photosystem II reaction center protein I ...Photosystem II reaction center protein M / Photosystem II 5 kDa protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / PSII 6.1 kDa protein / Uncharacterized protein / Uncharacterized protein / Photosystem II protein D1 / Chlorophyll a-b binding protein, chloroplastic / Photosystem II reaction center protein I / Photosystem II reaction center protein H / Photosystem II reaction center protein Z / Cytochrome b559 subunit beta / Photosystem II reaction center protein T / Photosystem II reaction center protein L / Cytochrome b559 subunit alpha / Photosystem II CP47 reaction center protein / Photosystem II D2 protein / Photosystem II reaction center protein K / Photosystem II reaction center protein Ycf12 / Photosystem II CP43 reaction center protein
Similarity search - Component
Biological speciesPicea abies (Norway spruce)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.785 Å
AuthorsKopecny D / Semchonok DA / Kouril R
Funding support Czech Republic, Germany, 9 items
OrganizationGrant numberCountry
Grant Agency of the Czech Republic21-05497S Czech Republic
Ministry of Education, Youth and Sports of the Czech RepublicCZ.02.1.01/0.0/0.0/16_019/0000827 Czech Republic
German Federal Ministry for Education and Research03Z22HN23 Germany
German Federal Ministry for Education and Research03Z22HI2 Germany
German Federal Ministry for Education and Research03COV04 Germany
German Research Foundation (DFG)391498659 Germany
German Research Foundation (DFG)RTG 2467 Germany
Ministry of Education, Youth and Sports of the Czech RepublicNo. CZ.02.1.01/0.0/0.0/18_046/0015974 Czech Republic
Ministry of Education, Youth and Sports of the Czech RepublicLM2018127 Czech Republic
CitationJournal: Nat Plants / Year: 2023
Title: Cryo-EM structure of a plant photosystem II supercomplex with light-harvesting protein Lhcb8 and α-tocopherol.
Authors: Monika Opatíková / Dmitry A Semchonok / David Kopečný / Petr Ilík / Pavel Pospíšil / Iva Ilíková / Pavel Roudnický / Sanja Ćavar Zeljković / Petr Tarkowski / Fotis L Kyrilis / ...Authors: Monika Opatíková / Dmitry A Semchonok / David Kopečný / Petr Ilík / Pavel Pospíšil / Iva Ilíková / Pavel Roudnický / Sanja Ćavar Zeljković / Petr Tarkowski / Fotis L Kyrilis / Farzad Hamdi / Panagiotis L Kastritis / Roman Kouřil /
Abstract: The heart of oxygenic photosynthesis is the water-splitting photosystem II (PSII), which forms supercomplexes with a variable amount of peripheral trimeric light-harvesting complexes (LHCII). Our ...The heart of oxygenic photosynthesis is the water-splitting photosystem II (PSII), which forms supercomplexes with a variable amount of peripheral trimeric light-harvesting complexes (LHCII). Our knowledge of the structure of green plant PSII supercomplex is based on findings obtained from several representatives of green algae and flowering plants; however, data from a non-flowering plant are currently missing. Here we report a cryo-electron microscopy structure of PSII supercomplex from spruce, a representative of non-flowering land plants, at 2.8 Å resolution. Compared with flowering plants, PSII supercomplex in spruce contains an additional Ycf12 subunit, Lhcb4 protein is replaced by Lhcb8, and trimeric LHCII is present as a homotrimer of Lhcb1. Unexpectedly, we have found α-tocopherol (α-Toc)/α-tocopherolquinone (α-TQ) at the boundary between the LHCII trimer and the inner antenna CP43. The molecule of α-Toc/α-TQ is located close to chlorophyll a614 of one of the Lhcb1 proteins and its chromanol/quinone head is exposed to the thylakoid lumen. The position of α-Toc in PSII supercomplex makes it an ideal candidate for the sensor of excessive light, as α-Toc can be oxidized to α-TQ by high-light-induced singlet oxygen at low lumenal pH. The molecule of α-TQ appears to shift slightly into the PSII supercomplex, which could trigger important structure-functional modifications in PSII supercomplex. Inspection of the previously reported cryo-electron microscopy maps of PSII supercomplexes indicates that α-Toc/α-TQ can be present at the same site also in PSII supercomplexes from flowering plants, but its identification in the previous studies has been hindered by insufficient resolution.
History
DepositionDec 21, 2022-
Header (metadata) releaseJul 26, 2023-
Map releaseJul 26, 2023-
UpdateAug 30, 2023-
Current statusAug 30, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16389.png

Downloads & links

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Map

FileDownload / File: emd_16389.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.96 Å/pix.
x 448 pix.
= 430.649 Å		0.96 Å/pix.
x 448 pix.
= 430.649 Å		0.96 Å/pix.
x 448 pix.
= 430.649 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.96127 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.886
Minimum - Maximum-3.770672 - 7.081017
Average (Standard dev.)0.0065747574 (±0.2099037)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 430.64896 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_16389_msk_1.map
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Half map: #1

Fileemd_16389_half_map_1.map
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Half map: #2

Fileemd_16389_half_map_2.map
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Sample components

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Entire : The C2S2-type PSII-LHCII supercomplex

EntireName: The C2S2-type PSII-LHCII supercomplex
Components
  • Complex: The C2S2-type PSII-LHCII supercomplex
    • Complex: Photosystem II core complex (C2)
      • Protein or peptide: Photosystem II protein D1
      • Protein or peptide: Photosystem II CP47 reaction center protein
      • Protein or peptide: Photosystem II CP43 reaction center protein
      • Protein or peptide: Photosystem II D2 protein
      • Protein or peptide: Cytochrome b559 subunit alpha
      • Protein or peptide: Cytochrome b559 subunit beta
      • Protein or peptide: Photosystem II reaction center protein H
      • Protein or peptide: Photosystem II reaction center protein I
      • Protein or peptide: Photosystem II reaction center protein K
      • Protein or peptide: Photosystem II reaction center protein L
      • Protein or peptide: Photosystem II reaction center protein M
      • Protein or peptide: Oxygen-evolving enhancer protein 1, chloroplastic
      • Protein or peptide: Photosystem II reaction center protein T
      • Protein or peptide: Photosystem II 5 kDa protein, chloroplastic
      • Protein or peptide: Photosystem II reaction center protein Ycf12
      • Protein or peptide: PSII 6.1 kDa protein
      • Protein or peptide: Photosystem II PsbX
      • Protein or peptide: Photosystem II reaction center protein Z
    • Complex: Photosystem II light-harvesting complex
      • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
      • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
      • Protein or peptide: Chlorophyll a-b binding protein, chloroplastic
  • Ligand: FE (II) ION
  • Ligand: CHLOROPHYLL A
  • Ligand: PHEOPHYTIN APheophytin
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE
  • Ligand: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: ALPHA-LINOLENIC ACID
  • Ligand: PALMITOLEIC ACID
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: MAGNESIUM ION
  • Ligand: (2R)-2,5,7,8-TETRAMETHYL-2-[(4R,8R)-4,8,12-TRIMETHYLTRIDECYL]CHROMAN-6-OL
  • Ligand: BICARBONATE IONBicarbonate
  • Ligand: DIACYL GLYCEROLDiglyceride
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: CHLOROPHYLL B
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: water

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Supramolecule #1: The C2S2-type PSII-LHCII supercomplex

SupramoleculeName: The C2S2-type PSII-LHCII supercomplex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#21
Source (natural)Organism: Picea abies (Norway spruce)

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Supramolecule #2: Photosystem II core complex (C2)

SupramoleculeName: Photosystem II core complex (C2) / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#6, #8-#13, #16-#21
Source (natural)Organism: Picea abies (Norway spruce)

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Supramolecule #3: Photosystem II light-harvesting complex

SupramoleculeName: Photosystem II light-harvesting complex / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #7, #14-#15
Source (natural)Organism: Picea abies (Norway spruce)

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Macromolecule #1: Photosystem II protein D1

MacromoleculeName: Photosystem II protein D1 / type: protein_or_peptide / ID: 1 / Details: PsbA / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem II
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 38.803215 KDa
SequenceString: MTAIIERRES ANLWGRFCDW ITSTENRLYI GWFGVLMIPT LLTATSVFII AFIAAPPVDI DGIREPVSGS LLYGNNIISG AIIPTSAAI GLHFYPIWEA ASVDEWLYNG GPYELIVLHF LLGVACYMGR EWELSFRLGM RPWIAVAYSA PVAAATAVFL I YPIGQGSF ...String:
MTAIIERRES ANLWGRFCDW ITSTENRLYI GWFGVLMIPT LLTATSVFII AFIAAPPVDI DGIREPVSGS LLYGNNIISG AIIPTSAAI GLHFYPIWEA ASVDEWLYNG GPYELIVLHF LLGVACYMGR EWELSFRLGM RPWIAVAYSA PVAAATAVFL I YPIGQGSF SDGMPLGISG TFNFMIVFQA EHNILMHPFH MLGVAGVFGG SLFSAMHGSL VTSSLIRETT ENQSANAGYK FG QEEETYN IVAAHGYFGR LIFQYASFNN SRSLHFFLAA WPVAGIWFTA LGISTMAFNL NGFNFNQSVV DSQGRVINTW ADI INRANL GMEVMHERNA HNFPLDLAAV ESISIGG

UniProtKB: Photosystem II protein D1

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Macromolecule #2: Photosystem II CP47 reaction center protein

MacromoleculeName: Photosystem II CP47 reaction center protein / type: protein_or_peptide / ID: 2 / Details: PsbB / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 56.375496 KDa
SequenceString: MGLPWYRVHT VVLNDPGRLI SVHIMHTALV AGWAGSMTLY ELAVFDPSDP VLDPMWRQGM FVIPFMTRLG IKDSWTGWNI TGETVINPG IWSYEGVAGA HIMFSGLCFL AAIWHWVYWD LEIFCDERTG KLCLDLPKVF GIHLFLSGVA CFGFGAFHVT G LYGPGIWV ...String:
MGLPWYRVHT VVLNDPGRLI SVHIMHTALV AGWAGSMTLY ELAVFDPSDP VLDPMWRQGM FVIPFMTRLG IKDSWTGWNI TGETVINPG IWSYEGVAGA HIMFSGLCFL AAIWHWVYWD LEIFCDERTG KLCLDLPKVF GIHLFLSGVA CFGFGAFHVT G LYGPGIWV SDPYGLTGKI QPVDPAWGAE GFDPFVPGGI ASHHIAAGIL GILAGLFHLS VRPPQRLYVG LRMGNIETVL SS SIAAVFF AAFVVAGTMW YGSATTPVEL FGPTRYQWDQ GYFQQEIDRR VRAGLAENLS LSEAWSKIPE KLAFYDYIGN NPA KGGLFR AGAMDNGDGI AVGWLGHPIF KDKEGNELFV RRMPTFFETF PVVLVDKEGI VKADVPFRRA ESKYSVEQVG VTVE FYGGG LDRVSFGDPA IVKKYARRAQ LGEIFELDRA TLKSDGVFRS SPRGWFTFGH ATFALLFFFG HIWHGARTLF RDVFA GIDP DLDARIEFGA FQKLGDPTTK RQVV

UniProtKB: Photosystem II CP47 reaction center protein

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Macromolecule #3: Photosystem II CP43 reaction center protein

MacromoleculeName: Photosystem II CP43 reaction center protein / type: protein_or_peptide / ID: 3 / Details: PsbC / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 51.78527 KDa
SequenceString: MKTLYSLRRS YPVETLFNGT IALAGRDQET TGFAWWAGNA RLINLSGKLL GAHVAHAGLI VFWAGAMNLF EVAHFVPEKP MYEQGLILL PHLATLGWGV GPGGEIVDTF PYFVSGVLHL ISSAVLGFGG IYHALIGPET LEESFPFFGY VWKDRNKMTT I LGIHLILL ...String:
MKTLYSLRRS YPVETLFNGT IALAGRDQET TGFAWWAGNA RLINLSGKLL GAHVAHAGLI VFWAGAMNLF EVAHFVPEKP MYEQGLILL PHLATLGWGV GPGGEIVDTF PYFVSGVLHL ISSAVLGFGG IYHALIGPET LEESFPFFGY VWKDRNKMTT I LGIHLILL GVGAFLLVLK ALYFGGVYDT WAPGGGDVRK ITNPTLNPSA IFGYLLKSPF GGEGWIVSVD NLEDVIGGHV WL GSICIFG GIWHILTKPF AWARRAFVWS GEAYLSYSLA ALSLFGFIAC CFVWFNNTAY PSEFYGPTGP EASQAQAFTF LVR DQRLGA SVGSAQGPTG LGKYLMRSPT GEIIFGGETM RFWDLRAPWL EPLRGPNGLD LSKLRKDIQP WQERRSAEYM THAP LGSSN SVGGVATEIN AVNYVSPRSW LATSHFVLGF FLFVGHLWHA GRARAAAAGF EKGIDRDFEP VLSMTPLN

UniProtKB: Photosystem II CP43 reaction center protein

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Macromolecule #4: Photosystem II D2 protein

MacromoleculeName: Photosystem II D2 protein / type: protein_or_peptide / ID: 4 / Details: PsbD / Number of copies: 2 / Enantiomer: LEVO / EC number: photosystem II
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 39.483117 KDa
SequenceString: MTVALGKSSK EEKTLFDTVD DWLRRDRFVF VGWSGLLLFP CAYFALGGWF TGTTFVTSWY THGLASSYLE GCNFLTAAVS TPANSLAHS LLLLWGPEAQ GDFTRWCQLG GLWTFVALHG AFGLIGFMLR QFELARSVQL RPYNAIAFSA PIAVFVPVFL I YPLGQSGW ...String:
MTVALGKSSK EEKTLFDTVD DWLRRDRFVF VGWSGLLLFP CAYFALGGWF TGTTFVTSWY THGLASSYLE GCNFLTAAVS TPANSLAHS LLLLWGPEAQ GDFTRWCQLG GLWTFVALHG AFGLIGFMLR QFELARSVQL RPYNAIAFSA PIAVFVPVFL I YPLGQSGW FFAPSFGVAA IFRFILFFQG FHNWTLNPFH MMGVAGVLGA ALLCAIHGAT VENTLFEDGD GANTFRAFNP TQ AEETYSM VTANRFWSQI FGVAFSNKRW LHFFMLFVPV TGLWMSAIGV VGLALNLRAY DFVSQEIRAA EDPEFETFYT KNI LLNEGI RAWMAAQDQP HENLIFPEEV LPRGNAL

UniProtKB: Photosystem II D2 protein

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Macromolecule #5: Cytochrome b559 subunit alpha

MacromoleculeName: Cytochrome b559 subunit alpha / type: protein_or_peptide / ID: 5 / Details: PsbE / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 9.478562 KDa
SequenceString:
MSGNTGERSF ADIITSIRYW VIHSITIPSL FIAGWLFVST GLAYDVFGSP RPNEYFTESR QEVPLVTGRF DSLEQLDEFT RSF

UniProtKB: Cytochrome b559 subunit alpha

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Macromolecule #6: Cytochrome b559 subunit beta

MacromoleculeName: Cytochrome b559 subunit beta / type: protein_or_peptide / ID: 6 / Details: PsbF / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 4.476271 KDa
SequenceString:
MTIDRTYPIF TVRWLAIHGL AVPTVFFSGS ISAMQFIQR

UniProtKB: Cytochrome b559 subunit beta

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Macromolecule #7: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 7 / Details: Lhcb1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 29.112004 KDa
SequenceString: MASCGIGSCA FAGGQISSLK PHNNQLLGVG AGVHGEARVT MRKSSTTKKV SASASPSPWY GPDRVLYLGP FSGEPPSYLT GEFPGDYGW DTAGLSADPE TFAKNRELEV IHSRWAMLGA LGCVFPELLA RNGVKFGEAV WFKAGAQIFS EGGLDYLGNP S LIHAQSIL ...String:
MASCGIGSCA FAGGQISSLK PHNNQLLGVG AGVHGEARVT MRKSSTTKKV SASASPSPWY GPDRVLYLGP FSGEPPSYLT GEFPGDYGW DTAGLSADPE TFAKNRELEV IHSRWAMLGA LGCVFPELLA RNGVKFGEAV WFKAGAQIFS EGGLDYLGNP S LIHAQSIL AIWACQVILM GAVEGYRIAG GPLGEITDPI YPGGSFDPLG LADDPDAFAE LKVKEIKNGR LAMFSMFGFF VQ AIVTGKG PLENLADHLA DPVNNNAWAY ATNFVPGK

UniProtKB: Chlorophyll a-b binding protein, chloroplastic

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Macromolecule #8: Photosystem II reaction center protein H

MacromoleculeName: Photosystem II reaction center protein H / type: protein_or_peptide / ID: 8 / Details: PsbH / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 7.941112 KDa
SequenceString:
MATQTIDDTS KTTPRATGVG TSLKPLNSEY GKVAPGWGTT PLMGFTMALF AVFLSIILEI YNSSVLLDGI PVSWG

UniProtKB: Photosystem II reaction center protein H

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Macromolecule #9: Photosystem II reaction center protein I

MacromoleculeName: Photosystem II reaction center protein I / type: protein_or_peptide / ID: 9 / Details: PsbI / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 4.184984 KDa
SequenceString:
MLTLKLFVYT VVIFFISLFI FGFLSNDPGR NPGRKE

UniProtKB: Photosystem II reaction center protein I

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Macromolecule #10: Photosystem II reaction center protein K

MacromoleculeName: Photosystem II reaction center protein K / type: protein_or_peptide / ID: 10 / Details: PsbK / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 6.804083 KDa
SequenceString:
MPVMLNIFLD DAFIYSNNIF FGKLPEAYAI FDPIVDVMPI IPVLFFLLAF VWQAAVSFR

UniProtKB: Photosystem II reaction center protein K

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Macromolecule #11: Photosystem II reaction center protein L

MacromoleculeName: Photosystem II reaction center protein L / type: protein_or_peptide / ID: 11 / Details: PsbL / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 4.475153 KDa
SequenceString:
MTQLNPNNQN VELNRTSLYW GLLLIFVLAV LFSNYLFN

UniProtKB: Photosystem II reaction center protein L

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Macromolecule #12: Photosystem II reaction center protein M

MacromoleculeName: Photosystem II reaction center protein M / type: protein_or_peptide / ID: 12 / Details: PsbM / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 3.952636 KDa
SequenceString:
MEVNTLAFIA VLLFLAISTA FLFILYVKTA SASSGNN

UniProtKB: Photosystem II reaction center protein M

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Macromolecule #13: Oxygen-evolving enhancer protein 1, chloroplastic

MacromoleculeName: Oxygen-evolving enhancer protein 1, chloroplastic / type: protein_or_peptide / ID: 13 / Details: PsbO / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 36.089457 KDa
SequenceString: MAASLATGSA LQAAATLLQP TKLSVTRTSS SRLSSSSSNL SRAFGFEPAT SARVSCSLQD DVKEFAEKCA GATKIAALAL AASSLVASG AGAEGVPKRL TYDEIQSQTY LEVKGSGTAN QCPTIDGGVE AFPFKPGKYE MKKLCMEPTS FTVKAEGTNK N QPPDFQKT ...String:
MAASLATGSA LQAAATLLQP TKLSVTRTSS SRLSSSSSNL SRAFGFEPAT SARVSCSLQD DVKEFAEKCA GATKIAALAL AASSLVASG AGAEGVPKRL TYDEIQSQTY LEVKGSGTAN QCPTIDGGVE AFPFKPGKYE MKKLCMEPTS FTVKAEGTNK N QPPDFQKT RLMTRLTYTL DEIEGPLEVS QDGKLKFEEK DGIDYAAVTV QLPGGERVPF LFTVKQLVAS GTPDSFSGQF LV PAYRGSS FLDPKGRGGS TGYDNAVALP AGGRGDEEEL VKENIKDVSA SKGEITFSVS KSKPDTGEVI GVFESLQPSD TDL GSKAPK DVKIQGIWYA QIEKP

UniProtKB: Uncharacterized protein

+
Macromolecule #14: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 14 / Details: Lhcb4.3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 32.767854 KDa
SequenceString: MASATAASSL ASASSSFFAG GQQLKAEKNV SRIYARFSFS GLKKKTKAVA KPKTKAVAKP KTKAVAKPKP KPRAPTGRPL WLPGAKAPE WLDGSLVGDY GFDPLGLGKP SEYLQYDVDS LDQNLDLNLP GDLLGDFVGD PDIKPSIQPY SEVFGLQRFR E CELIHGRW ...String:
MASATAASSL ASASSSFFAG GQQLKAEKNV SRIYARFSFS GLKKKTKAVA KPKTKAVAKP KTKAVAKPKP KPRAPTGRPL WLPGAKAPE WLDGSLVGDY GFDPLGLGKP SEYLQYDVDS LDQNLDLNLP GDLLGDFVGD PDIKPSIQPY SEVFGLQRFR E CELIHGRW AMLGVLGALA VEGFTGIYWQ DAGKVELVEG SSYFGFSLPF NMSTLILIEV LLLGYIEFQR NAEVDPETRL YP GGKYFDP FGLAVDEIKK DRLKLAEIKH ARLAMVAFLI FAIQAAVTSK GPLTLFVEFL GKK

UniProtKB: Chlorophyll a-b binding protein, chloroplastic

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Macromolecule #15: Chlorophyll a-b binding protein, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein, chloroplastic / type: protein_or_peptide / ID: 15 / Details: CP26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 32.287926 KDa
SequenceString: MASIAALGST AALGRADQML GNPLNMNMLA ASTAASRSST RLTSPSLQVV SLFGFKKKAA APPPPSKPKA KAAAANPASE ELAKWYGPD RRIFLPEGLL DRADIPEYLN GEVPGDYGYD PFGLSKKPEN FDKYQAYELI HARWAMLGAA GFIIPEAFNK F GAYCGPEA ...String:
MASIAALGST AALGRADQML GNPLNMNMLA ASTAASRSST RLTSPSLQVV SLFGFKKKAA APPPPSKPKA KAAAANPASE ELAKWYGPD RRIFLPEGLL DRADIPEYLN GEVPGDYGYD PFGLSKKPEN FDKYQAYELI HARWAMLGAA GFIIPEAFNK F GAYCGPEA VWFKTGALLL DGGTLSYFGA SIPINLIAAV VAEVILVGGA EYYRLTNGLD FEDKLHPGGP FDPLGLAKDP DQ FALLKVK EIKNGRLAMF SMLGFFVQAY VTGEGPVENL AAHLSDPFGN NLLTVLGGSL ERAPTL

UniProtKB: Chlorophyll a-b binding protein, chloroplastic

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Macromolecule #16: Photosystem II reaction center protein T

MacromoleculeName: Photosystem II reaction center protein T / type: protein_or_peptide / ID: 16 / Details: PsbTc / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 3.962781 KDa
SequenceString:
MEALVYTFLL VSTLGIIFFA IFFREPPKIP NKGGK

UniProtKB: Photosystem II reaction center protein T

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Macromolecule #17: Photosystem II 5 kDa protein, chloroplastic

MacromoleculeName: Photosystem II 5 kDa protein, chloroplastic / type: protein_or_peptide / ID: 17 / Details: PsbTn / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 14.189462 KDa
SequenceString:
MASLSLCAPC NISSASSLAA GYNKVPCKSV RGGAQVGQVF MVNKPFKASQ DWAVHDENVT MKKKEDDQER MQRRRMMFTA AAAAVSAAA SQGMMAMAAG EKPTGPEPKR GTPEAKKLYA RVCVTMPTAS VCHN

UniProtKB: Photosystem II 5 kDa protein, chloroplastic

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Macromolecule #18: Photosystem II reaction center protein Ycf12

MacromoleculeName: Photosystem II reaction center protein Ycf12 / type: protein_or_peptide / ID: 18 / Details: Psb30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 3.49029 KDa
SequenceString:
MNLEVIAQLT VLTLTVVSGP LVIVLLAVRK GNL

UniProtKB: Photosystem II reaction center protein Ycf12

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Macromolecule #19: PSII 6.1 kDa protein

MacromoleculeName: PSII 6.1 kDa protein / type: protein_or_peptide / ID: 19 / Details: PsbW / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 15.266507 KDa
SequenceString:
MAATAVFSTT TSVSAFLLNR SLGSAAASRP SSLSVVGLPA LDKRCTVCYA QKQEEIVRAK ENSQNCMVSL GGAAPLMAVA AALTVSTKE AFALVDERMS TEGTGLGLGL SNPLLGWILV GVFTLIWILY FTTYSGVKED EDSGLSL

UniProtKB: PSII 6.1 kDa protein

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Macromolecule #20: Photosystem II PsbX

MacromoleculeName: Photosystem II PsbX / type: protein_or_peptide / ID: 20 / Details: PsbX / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 12.95694 KDa
SequenceString:
MASATAISLA LPMATASSVS QRDALKAKHA HGSSLFTTPN KLYSPKLASN RSRVVMHASQ GKKEQSLAGL TSLALAAAMA IPEIAEAAG SGVSPSLKNF LLSIAAGGVV ATAIAGAVIG VSNFDPVKRN

UniProtKB: Uncharacterized protein

+
Macromolecule #21: Photosystem II reaction center protein Z

MacromoleculeName: Photosystem II reaction center protein Z / type: protein_or_peptide / ID: 21 / Details: PsbZ / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Picea abies (Norway spruce)
Molecular weightTheoretical: 6.412558 KDa
SequenceString:
MTIAFQSAVF ALIAISLLLV IGVPVALASP DGWSSSKNVV FSGVSLWIGS VLFVGILNSF IS

UniProtKB: Photosystem II reaction center protein Z

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Macromolecule #22: FE (II) ION

MacromoleculeName: FE (II) ION / type: ligand / ID: 22 / Number of copies: 2 / Formula: FE2
Molecular weightTheoretical: 55.845 Da

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Macromolecule #23: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 23 / Number of copies: 154 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

+
Macromolecule #24: PHEOPHYTIN A

MacromoleculeName: PHEOPHYTIN A / type: ligand / ID: 24 / Number of copies: 4 / Formula: PHO
Molecular weightTheoretical: 871.2 Da
Chemical component information

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

+
Macromolecule #25: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 25 / Number of copies: 20 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #26: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 26 / Number of copies: 4 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Macromolecule #27: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 27 / Number of copies: 12 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

+
Macromolecule #28: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

MacromoleculeName: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / type: ligand / ID: 28 / Number of copies: 12 / Formula: 3PH
Molecular weightTheoretical: 704.998 Da
Chemical component information

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / Phosphatidic acid

+
Macromolecule #29: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...

MacromoleculeName: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE
type: ligand / ID: 29 / Number of copies: 4 / Formula: PL9
Molecular weightTheoretical: 749.201 Da
Chemical component information

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

+
Macromolecule #30: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 30 / Number of copies: 18 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

+
Macromolecule #31: ALPHA-LINOLENIC ACID

MacromoleculeName: ALPHA-LINOLENIC ACID / type: ligand / ID: 31 / Number of copies: 26 / Formula: LNL
Molecular weightTheoretical: 278.43 Da
Chemical component information

ChemComp-LNL:
ALPHA-LINOLENIC ACID / Alpha-Linolenic acid

+
Macromolecule #32: PALMITOLEIC ACID

MacromoleculeName: PALMITOLEIC ACID / type: ligand / ID: 32 / Number of copies: 2 / Formula: PAM
Molecular weightTheoretical: 254.408 Da
Chemical component information

ChemComp-PAM:
PALMITOLEIC ACID / Palmitoleic acid

+
Macromolecule #33: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 33 / Number of copies: 4 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

+
Macromolecule #34: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 34 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #35: (2R)-2,5,7,8-TETRAMETHYL-2-[(4R,8R)-4,8,12-TRIMETHYLTRIDECYL]CHRO...

MacromoleculeName: (2R)-2,5,7,8-TETRAMETHYL-2-[(4R,8R)-4,8,12-TRIMETHYLTRIDECYL]CHROMAN-6-OL
type: ligand / ID: 35 / Number of copies: 2 / Formula: VIV
Molecular weightTheoretical: 430.706 Da
Chemical component information

ChemComp-VIV:
(2R)-2,5,7,8-TETRAMETHYL-2-[(4R,8R)-4,8,12-TRIMETHYLTRIDECYL]CHROMAN-6-OL / Α-Tocopherol

+
Macromolecule #36: BICARBONATE ION

MacromoleculeName: BICARBONATE ION / type: ligand / ID: 36 / Number of copies: 2 / Formula: BCT
Molecular weightTheoretical: 61.017 Da
Chemical component information

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

+
Macromolecule #37: DIACYL GLYCEROL

MacromoleculeName: DIACYL GLYCEROL / type: ligand / ID: 37 / Number of copies: 2 / Formula: DGA
Molecular weightTheoretical: 625.018 Da
Chemical component information

ChemComp-DGA:
DIACYL GLYCEROL / Diglyceride

+
Macromolecule #38: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 38 / Number of copies: 2 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

+
Macromolecule #39: CHLOROPHYLL B

MacromoleculeName: CHLOROPHYLL B / type: ligand / ID: 39 / Number of copies: 50 / Formula: CHL
Molecular weightTheoretical: 907.472 Da
Chemical component information

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

+
Macromolecule #40: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 40 / Number of copies: 18 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

+
Macromolecule #41: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...

MacromoleculeName: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
type: ligand / ID: 41 / Number of copies: 10 / Formula: NEX
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

+
Macromolecule #42: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 42 / Number of copies: 8 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

+
Macromolecule #43: water

MacromoleculeName: water / type: ligand / ID: 43 / Number of copies: 280 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration3 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
10.0 mMC8H18N2O4SHEPES
0.008 %C24H46O11n-dodecyl alpha-D-maltoside
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 25 sec. / Pretreatment - Atmosphere: AIR / Details: PELCO easiGlow, 15 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details3 mg of chlorophylls/ml

-
Electron microscopy

MicroscopeTFS GLACIOS
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: OTHER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 2392 / Average electron dose: 120.0 e/Å2

-
Image processing

Particle selectionNumber selected: 202251
Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.0) / Software - details: ab-initio
Final 3D classificationNumber classes: 2 / Avg.num./class: 55000 / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.0)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C2 (2 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.785 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.0) / Number images used: 60956
FSC plot (resolution estimation)

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-8c29:
Cryo-EM structure of photosystem II C2S2 supercomplex from Norway spruce (Picea abies) at 2.8 Angstrom resolution

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