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- EMDB-1482: Structrue of truncated portal(portal602) complex with gp4 from ba... -

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Basic information

Entry
Database: EMDB / ID: 1482
TitleStructrue of truncated portal(portal602) complex with gp4 from bacteriophage P22
Sampletruncated portal complex with gp4 from bacteriophage P22
SourceEnterobacteria phage P22 / virus
Map datamap of portal602 complex with gp4
Methodsingle particle reconstruction, at 8.5 A resolution
AuthorsZheng H / Olia AS / Gonen M / Andrews S / Cingolani G / Gonen T
CitationMol. Cell, 2008, 29, 376-383

Mol. Cell, 2008, 29, 376-383 StrPapers
A conformational switch in bacteriophage p22 portal protein primes genome injection.
Hongjin Zheng / Adam S Olia / Melissa Gonen / Simeon Andrews / Gino Cingolani / Tamir Gonen

DateDeposition: Feb 26, 2008 / Header (metadata) release: Feb 26, 2008 / Map release: Mar 31, 2009 / Last update: Feb 26, 2008

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3.850629391
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 3.850629391
  • Imaged by UCSF CHIMERA
  • Download
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Supplemental images

Downloads & links

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Map

Fileemd_1482.map.gz (map file in CCP4 format, 6751 KB)
Projections & slices
Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
120 pix
2.48 A/pix
= 297.6 A
120 pix
2.48 A/pix
= 297.6 A
120 pix
2.48 A/pix
= 297.6 A

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 2.48 A
Density
Contour Level:2.75, 3.8506294 (movie #1):
Minimum - Maximum-2.59705 - 7.69801
Average (Standard dev.)-7.13014e-09 (1)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions120120120
Origin-60-60-60
Limit595959
Spacing120120120
CellA=B=C: 297.6 A
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z2.482.482.48
M x/y/z120120120
origin x/y/z0.0000.0000.000
length x/y/z297.600297.600297.600
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ-55-55-55
NX/NY/NZ111111111
MAP C/R/S123
start NC/NR/NS-60-60-60
NC/NR/NS120120120
D min/max/mean-2.5977.698-0.000

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Supplemental data

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Sample components

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Entire truncated portal complex with gp4 from bacteriophage P22

EntireName: truncated portal complex with gp4 from bacteriophage P22
Number of components: 2 / Oligomeric State: 12mer of portal with 12mer of gp4

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Component #1: protein, gp1, gp4

ProteinName: gp1, gp4 / a.k.a: gp1, gp4 / Recombinant expression: Yes
SourceSpecies: Enterobacteria phage P22 / virus

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Experimental details

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Sample preparation

Specimen stateparticle
VitrificationInstrument: NONE / Cryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: OTHER
LensImaging mode: OTHER
Specimen HolderHolder: Eucentric / Model: OTHER

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Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C12 (12 fold cyclic)
3D reconstructionResolution: 8.5 A / Resolution method: FSC 0.143

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