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- EMDB-1467: Human Parvovirus B19 (empty wildtype particle) -

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Basic information

Database: EMDB / ID: 1467
TitleHuman Parvovirus B19 (empty wildtype particle)
Keywordsicosahedral / empty viral particle / B19
SampleHuman Parvovirus B19 (empty wildtype particle)
SourceHuman parvovirus B19 / virus
Map dataHuman Parvovirus B19 (eB19, empty wildtype particle)
Methodsingle particle (icosahedral) reconstruction, at 11.3 A resolution
AuthorsKaufmann B / Chipman PR / Modrow S / Rossmann MG
CitationJ. Virol., 2008, 82, 7306-7312

J. Virol., 2008, 82, 7306-7312 StrPapers
Visualization of the externalized VP2 N termini of infectious human parvovirus B19.
Bärbel Kaufmann / Paul R Chipman / Victor A Kostyuchenko / Susanne Modrow / Michael G Rossmann

DateDeposition: Feb 5, 2008 / Header (metadata) release: Feb 7, 2008 / Map release: Mar 31, 2009 / Last update: Feb 5, 2008

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 18
  • Imaged by UCSF CHIMERA
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  • Surface view colored by radius
  • Surface level: 18
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

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Supplemental images

Downloads & links


Fileemd_1467.map.gz (map file in CCP4 format, 5344 KB)
Projections & slicesSize of images:
AxesZ (Sec.)X (Row.)Y (Col.)
111 pix
2.82 A/pix
= 312.63 A
111 pix
2.82 A/pix
= 312.63 A
111 pix
2.82 A/pix
= 312.63 A



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 2.81649 A
Contour Level:11, 18 (movie #1):
Minimum - Maximum-10.31 - 34.561
Average (Standard dev.)2.24292 (8.7631)


Space Group Number1
Map Geometry
Axis orderYXZ
CellA=B=C: 312.63 A
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z2.81648648648652.81648648648652.8164864864865
M x/y/z111111111
origin x/y/z0.0000.0000.000
length x/y/z312.630312.630312.630
start NX/NY/NZ-55-55-55
MAP C/R/S213
start NC/NR/NS-55-55-55
D min/max/mean-10.31034.5612.243

Supplemental data

Sample components

Entire Human Parvovirus B19 (empty wildtype particle)

EntireName: Human Parvovirus B19 (empty wildtype particle)
Details: icosahedral, empty viral particle, non-enveloped protein shell
Number of components: 1
Oligomeric State: 60 VP subunits form icosahedral protein shell
MassTheoretical: 3.6 MDa

Component #1: virus, Human parvovirus B19

VirusName: Human parvovirus B19 / a.k.a: Human Parvovirus B19 / Class: OTHER
Details: empty wildtype particles purified from human plasma, no ssDNA genome encapsidated
Empty: Yes / Enveloped: No / Isolate: SPECIES
MassTheoretical: 3.6 MDa
SpeciesSpecies: Human parvovirus B19 / virus
Source (natural)Host Species: Homo sapiens / human / Host category: VERTEBRATES

Experimental details

Sample preparation

Specimen stateparticle
Sample solutionSpecimen conc.: 1 mg/ml / Buffer solution: 25mM Tris-HCl / pH: 7.5
Support film400 mesh copper
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: ETHANE
Method: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.
Details: Vitrification instrument: guillotine-style plunge freezing device

Electron microscopy imaging

ImagingMicroscope: FEI/PHILIPS CM300FEG/T / Details: low dose
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 22 e/A2 / Illumination mode: FLOOD BEAM
LensMagnification: 45000 X (nominal), 47000 X (calibrated) / Astigmatism: live FFT at 200K / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1250 - 3660 nm
Specimen HolderHolder: Eucentric / Model: GATAN LIQUID NITROGEN / Temperature: 98 K
CameraDetector: KODAK SO-163 FILM

Image acquisition

Image acquisitionNumber of digital images: 31 / Scanner: ZEISS SCAI / Sampling size: 14 microns / Bit depth: 8

Image processing

ProcessingMethod: single particle (icosahedral) reconstruction / Details: manual particle selection / Number of projections: 1959 / Applied symmetry: I (icosahedral)
3D reconstructionAlgorithm: model-based approach using Fourier methods / Software: EMPFT, POR, P3DR / CTF correction: each particle
Details: final map includes data to 10.5 Ang resolution (fsc 0.3 cut-off), magnification of final map standardized to a map calculated from B19 VP2 VLP model coordinates (PDB accession no 1S58) resulting in final pixel separation of 2.8165Ang
Resolution: 11.3 A / Resolution method: FSC 0.5

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