- EMDB-1450: Human T-lymphotropic virus-1 visualized at the virological synaps... -
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Basic information
Entry
Database: EMDB / ID: EMD-1450
Title
Human T-lymphotropic virus-1 visualized at the virological synapse by electron tomography.
Map data
The tomogram shows a virological synapse between a naturally HTVL-1 infected CD4+ T lymphocyte (right) and a target cell (left). Two electron dense (anti-gag labeled) HTLV-1 particles can be seen in the synaptic cleft.
Sample
Sample: HTLV-1 virological snapse formed between CD4 T lymphocytes of HTLV-1 infeceted individual
Organelle or cellular component: virological synapse
Biological species
Homo sapiens (human)
Method
electron tomography / negative staining / Resolution: 50.0 Å
Journal: PLoS One / Year: 2008 Title: Human T-lymphotropic virus-1 visualized at the virological synapse by electron tomography. Authors: Endre Majorovits / Mohamed Nejmeddine / Yuetsu Tanaka / Graham P Taylor / Stephen D Fuller / Charles R M Bangham / Abstract: Human T-lymphotropic virus 1 (HTLV-1) is transmitted directly between cells via an organized cell-cell contact called a virological synapse (VS). The VS has been studied by light microscopy, but the ...Human T-lymphotropic virus 1 (HTLV-1) is transmitted directly between cells via an organized cell-cell contact called a virological synapse (VS). The VS has been studied by light microscopy, but the ultrastructure of the VS and the nature of the transmitted viral particle have remained unknown. Cell-free enveloped virions of HTLV-1 are undetectable in the serum of individuals infected with the human T-lymphotropic virus 1 (HTLV-1) and during in vitro culture of naturally infected lymphocytes. However, the viral envelope protein is required for infectivity of HTLV-1, suggesting that complete, enveloped HTLV-1 virions are transferred across the synapse. Here, we use electron tomography combined with immunostaining of viral protein to demonstrate the presence of enveloped HTLV-1 particles within the VS formed between naturally infected lymphocytes. We show in 3D that HTLV-1 particles can be detected in multiple synaptic clefts at different locations simultaneously within the same VS. The synaptic clefts are surrounded by the tightly apposed plasma membranes of the two cells. HTLV-1 virions can contact the recipient cell membrane before detaching from the infected cell. The results show that the HTLV-1 virological synapse that forms spontaneously between lymphocytes of HTLV-1 infected individuals allows direct cell-cell transmission of the virus by triggered, directional release of enveloped HTLV-1 particles into confined intercellular spaces.
Download / File: emd_1450.map.gz / Format: CCP4 / Size: 156.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
The tomogram shows a virological synapse between a naturally HTVL-1 infected CD4+ T lymphocyte (right) and a target cell (left). Two electron dense (anti-gag labeled) HTLV-1 particles can be seen in the synaptic cleft.
Voxel size
X: 33.6 Å / Y: 33.6 Å / Z: 25.9 Å
Density
Minimum - Maximum
-128.0 - 127.0
Average (Standard dev.)
17.9574 (±4.31085)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
1300
600
215
Spacing
1300
600
215
Cell
A: 43680 Å / B: 20160 Å / C: 5568.5 Å α=β=γ: 90 °
CCP4 map header:
mode
envelope stored as signed bytes (from -128 lowest to 127 highest)
Å/pix. X/Y/Z
33.599996923077
33.6
25.9
M x/y/z
1300
600
215
origin x/y/z
0.000
0.000
0.000
length x/y/z
43679.996
20160.000
5568.500
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-60
-60
-59
NX/NY/NZ
120
120
120
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
600
1300
215
D min/max/mean
-128.000
127.000
17.957
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Supplemental data
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Sample components
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Entire : HTLV-1 virological snapse formed between CD4 T lymphocytes of HTL...
Entire
Name: HTLV-1 virological snapse formed between CD4 T lymphocytes of HTLV-1 infeceted individual
Components
Sample: HTLV-1 virological snapse formed between CD4 T lymphocytes of HTLV-1 infeceted individual
Organelle or cellular component: virological synapse
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Supramolecule #1000: HTLV-1 virological snapse formed between CD4 T lymphocytes of HTL...
Supramolecule
Name: HTLV-1 virological snapse formed between CD4 T lymphocytes of HTLV-1 infeceted individual type: sample / ID: 1000 / Number unique components: 1
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Supramolecule #1: virological synapse
Supramolecule
Name: virological synapse / type: organelle_or_cellular_component / ID: 1 / Number of copies: 1 / Recombinant expression: No / Database: NCBI
Source (natural)
Organism: Homo sapiens (human) / synonym: human being / Cell: CD4 T lymphocyte / Organelle: Plasma membrane / Location in cell: Plasma membrane
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Experimental details
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Structure determination
Method
negative staining
Processing
electron tomography
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Sample preparation
Buffer
pH: 7.2 / Details: sodium cacodylate buffer 0.1M
Staining
Type: NEGATIVE / Details: 1% OsO4 staining
Grid
Details: Formvar coated 2x1mm Cu slot grid
Vitrification
Cryogen name: NONE
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Electron microscopy
Microscope
FEI TECNAI F30
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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