Journal: Nature / Year: 2020 Title: The native structure of the assembled matrix protein 1 of influenza A virus. Authors: Julia Peukes / Xiaoli Xiong / Simon Erlendsson / Kun Qu / William Wan / Leslie J Calder / Oliver Schraidt / Susann Kummer / Stefan M V Freund / Hans-Georg Kräusslich / John A G Briggs / Abstract: Influenza A virus causes millions of severe cases of disease during annual epidemics. The most abundant protein in influenza virions is matrix protein 1 (M1), which mediates virus assembly by ...Influenza A virus causes millions of severe cases of disease during annual epidemics. The most abundant protein in influenza virions is matrix protein 1 (M1), which mediates virus assembly by forming an endoskeleton beneath the virus membrane. The structure of full-length M1, and how it oligomerizes to mediate the assembly of virions, is unknown. Here we determine the complete structure of assembled M1 within intact virus particles, as well as the structure of M1 oligomers reconstituted in vitro. We find that the C-terminal domain of M1 is disordered in solution but can fold and bind in trans to the N-terminal domain of another M1 monomer, thus polymerizing M1 into linear strands that coat the interior surface of the membrane of the assembling virion. In the M1 polymer, five histidine residues-contributed by three different monomers of M1-form a cluster that can serve as the pH-sensitive disassembly switch after entry into a target cell. These structures therefore reveal mechanisms of influenza virus assembly and disassembly.
History
Deposition
May 26, 2020
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Header (metadata) release
Oct 14, 2020
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Map release
Oct 14, 2020
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Update
Dec 2, 2020
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Current status
Dec 2, 2020
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Name: Influenza A virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1 / NCBI-ID: 11320 / Sci species name: Influenza A virus / Sci species strain: A/Hong Kong/1/1968 (H3N2) / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: Yes / Virus empty: No
Host (natural)
Organism: Homo sapiens (human)
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Supramolecule #2: Matrix protein 1
Supramolecule
Name: Matrix protein 1 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 Details: The M1 map was generated by subtomogram averaging of the M1 protein density in tomograms of influenza A virions.
Source (natural)
Organism: Influenza A virus / Strain: A/Hong Kong/1/1968 (H3N2)
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Experimental details
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Structure determination
Method
cryo EM
Processing
subtomogram averaging
Aggregation state
particle
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Sample preparation
Buffer
pH: 7.5
Grid
Model: Quantifoil R2/2 / Material: GOLD / Mesh: 200 / Support film - Material: CARBON
Vitrification
Cryogen name: ETHANE / Chamber humidity: 95 % / Instrument: LEICA EM GP
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Electron microscopy
Microscope
FEI TITAN KRIOS
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 3.2 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
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Image processing
Extraction
Number tomograms: 5 / Number images used: 100000 Method: Volumes picked semi-automatically along the particles membrane Software: (Name: TOM, MATLAB, UCSF Chimera) Details: Initial positions were generated by simulating the surface of a cylindricated volume around the manually determined central axis of each particle. Initial positions have been 2X oversampled.
CTF correction
Software: (Name: CTFFIND, NOVACTF) Details: CTF determination was performed using CTFFIND4. CTF correction was performed using 3D-CTF correction by CTF multiplication in NovaCTF.
Final angle assignment
Type: OTHER
Final reconstruction
Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 8.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 12128
FSC plot (resolution estimation)
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