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- EMDB-0270: Stringent response control by a bifunctional RelA enzyme in the p... -

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Basic information

Entry
Database: EMDB / ID: EMD-0270
TitleStringent response control by a bifunctional RelA enzyme in the presence and absence of the ribosome
Map data
Sample
  • Complex: Bacillus subtilis subsp. subtilis str. 168
    • RNA: x 6 types
    • Protein or peptide: x 47 types
Function / homology
Function and homology information


GTP diphosphokinase activity / guanosine tetraphosphate biosynthetic process / GTP diphosphokinase / positive regulation of rRNA processing / nucleoid / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit ...GTP diphosphokinase activity / guanosine tetraphosphate biosynthetic process / GTP diphosphokinase / positive regulation of rRNA processing / nucleoid / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / kinase activity / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / phosphorylation / response to antibiotic / mRNA binding / GTP binding / DNA binding / RNA binding / zinc ion binding / ATP binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
RelA/SpoT, AH and RIS domains / RelA/SpoT, AH and RIS domains / HD domain / RelA/SpoT family / RelA/SpoT, TGS domain / ACT domain / Region found in RelA / SpoT proteins / RelA/SpoT / Region found in RelA / SpoT proteins / TGS domain ...RelA/SpoT, AH and RIS domains / RelA/SpoT, AH and RIS domains / HD domain / RelA/SpoT family / RelA/SpoT, TGS domain / ACT domain / Region found in RelA / SpoT proteins / RelA/SpoT / Region found in RelA / SpoT proteins / TGS domain / ACT domain profile. / ACT domain / ACT-like domain / HD domain profile. / TGS domain profile. / TGS / TGS-like / HD domain / Ribosomal protein S14, type Z / Beta-grasp domain superfamily / Metal dependent phosphohydrolases with conserved 'HD' motif. / HD/PDEase domain / Ribosomal protein L31 type A / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Nucleotidyltransferase superfamily / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16
Similarity search - Domain/homology
Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / GTP pyrophosphokinase / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 ...Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / GTP pyrophosphokinase / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31
Similarity search - Component
Biological speciesBacillus subtilis subsp. subtilis str. 168 (bacteria) / Bacillus subtilis (strain 168) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.5 Å
AuthorsWilson DN / Abdelshahid M
CitationJournal: Cell Rep / Year: 2020
Title: Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel.
Authors: Patrick Pausch / Maha Abdelshahid / Wieland Steinchen / Heinrich Schäfer / Fabio Lino Gratani / Sven-Andreas Freibert / Christiane Wolz / Kürşad Turgay / Daniel N Wilson / Gert Bange /
Abstract: The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain ...The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain (NTD) harboring the second messenger nucleotide (p)ppGpp hydrolase and synthetase activity and a stress-perceiving and regulatory C-terminal domain (CTD). CTD-mediated binding of Rel to stalled ribosomes boosts (p)ppGpp synthesis. However, how the opposing activities of the NTD are controlled in the absence of stress was poorly understood. Here, we demonstrate on the RSH-type protein Rel that the critical regulative elements reside within the TGS (ThrRS, GTPase, and SpoT) subdomain of the CTD, which associates to and represses the synthetase to concomitantly allow for activation of the hydrolase. Furthermore, we show that Rel forms homodimers, which appear to control the interaction with deacylated-tRNA, but not the enzymatic activity of Rel. Collectively, our study provides a detailed molecular view into the mechanism of stringent response repression in the absence of stress.
History
Header (metadata) releaseAug 22, 2018-
DepositionOct 4, 2018-
Map releaseOct 23, 2019-
UpdateSep 30, 2020-
Current statusSep 30, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0471
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.0471
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6htq
  • Surface level: 0.0471
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0270.png

Downloads & links

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Map

FileDownload / File: emd_0270.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.084 Å
Density
Contour LevelBy AUTHOR: 0.0471 / Movie #1: 0.0471
Minimum - Maximum-0.3112882 - 0.46533
Average (Standard dev.)0.0002610036 (±0.01799019)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 390.24 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0841.0841.084
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z390.240390.240390.240
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.3110.4650.000

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Supplemental data

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Sample components

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Entire : Bacillus subtilis subsp. subtilis str. 168

EntireName: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Components
  • Complex: Bacillus subtilis subsp. subtilis str. 168
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 1Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: P-tRNA
    • Protein or peptide: GTP pyrophosphokinase
    • RNA: A/R-tRNA
    • RNA: E-tRNA

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Supramolecule #1: Bacillus subtilis subsp. subtilis str. 168

SupramoleculeName: Bacillus subtilis subsp. subtilis str. 168 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

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Macromolecule #1: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 948.665688 KDa
SequenceString: GUUAAGUUAG AAAGGGCGCA CGGUGGAUGC CUUGGCACUA GGAGCCGAUG AAGGACGGGA CGAACACCGA UAUGCUUCGG GGAGCUGUA AGCAAGCUUU GAUCCGGAGA UUUCCGAAUG GGGAAACCCA CCACUCGUAA UGGAGUGGUA UCCAUAUCUG A AUUCAUAG ...String:
GUUAAGUUAG AAAGGGCGCA CGGUGGAUGC CUUGGCACUA GGAGCCGAUG AAGGACGGGA CGAACACCGA UAUGCUUCGG GGAGCUGUA AGCAAGCUUU GAUCCGGAGA UUUCCGAAUG GGGAAACCCA CCACUCGUAA UGGAGUGGUA UCCAUAUCUG A AUUCAUAG GAUAUGAGAA GGCAGACCCG GGGAACUGAA ACAUCUAAGU ACCCGGAGGA AGAGAAAGCA AAUGCGAUUC CC UGAGUAG CGGCGAGCGA AACGGGAUCA GCCCAAACCA AGAGGCUUGC CUCUUGGGGU UGUAGGACAC UCUGUACGGA GUU ACAAAG GAACGAGGUA GAUGAAGAGG UCUGGAAAGG CCCGCCAUAG GAGGUAACAG CCCUGUAGUC AAAACUUCGU UCUC UCCUG AGUGGAUCCU GAGUACGGCG GAACACGUGA AAUUCCGUCG GAAUCCGGGA GGACCAUCUC CCAAGGCUAA AUACU CCCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAGAGAUC CUGAAA CCG UGUGCCUACA AGUAGUCAGA GCCCGUUAAC GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUCUC GU GCAAGGUUAA GCAGAAGAUG CGGAGCCGCA GCGAAAGCGA GUCUGAAUAG GGCGCAUGAG UACGUGGUCG UAGACCCG A AACCAGGUGA UCUACCCAUG UCCAGGGUGA AGUUCAGGUA ACACUGAAUG GAGGCCCGAA CCCACGCACG UUGAAAAGU GCGGGGAUGA GGUGUGGGUA GGGGUGAAAU GCCAAUCGAA CCUGGAGAUA GCUGGUUCUC UCCGAAAUAG CUUUAGGGCU AGCCUCAAG GUAAGAGUCU UGGAGGUAGA GCACUGAUUG GACUAGGGGC CCCUACCGGG UUACCGAAUU CAGUCAAACU C CGAAUGCC AAUGACUUAU CCUUGGGAGU CAGACUGCGA GUGAUAAGAU CCGUAGUCGA AAGGGAAACA GCCCAGACCG CC AGCUAAG GUCCCAAAGU AUACGUUAAG UGGAAAAGGA UGUGGAGUUG CUUAGACAAC CAGGAUGUUG GCUUAGAAGC AGC CACCAU UUAAAGAGUG CGUAAUAGCU CACUGGUCGA GUGACUCUGC GCCGAAAAUG UACCGGGGCU AAACGUAUCA CCGA AGCUG CGGACUGUUC UUCGAACAGU GGUAGGAGAG CGUUCUAAGG GCUGUGAAGC CAGACCGGAA GGACUGGUGG AGCGC UUAG AAGUGAGAAU GCCGGUAUGA GUAGCGAAAG AGGGGUGAGA AUCCCCUCCA CCGAAUGCCU AAGGUUUCCU GAGGAA GGC UCGUCCGCUC AGGGUUAGUC GGGACCUAAG CCGAGGCCGA AAGGCGUAGG CGAUGGACAA CAGGUUGAUA UUCCUGU AC CACCUCCUCA CCAUUUGAGC AAUGGGGGGA CGCAGGAGGA UAGGGUAAGC GCGGUAUUGG AUAUCCGCGU CCAAGCAG U UAGGCUGGGA AAUAGGCAAA UCCGUUUCCC AUAAGGCUGA GCUGUGAUGG CGAGCGAAAU AUAGUAGCGA AGUUCCUGA UUCCACACUG CCAAGAAAAG CCUCUAGCGA GGUGAGAGGU GCCCGUACCG CAAACCGACA CAGGUAGGCG AGGAGAGAAU CCUAAGGUG AUCGAGAGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUGUUA G GGUGCAAG CCCGAGAGAG CCGCAGUGAA UAGGCCCAGG CGACUGUUUA GCAAAAACAC AGGUCUCUGC GAAGCCGUAA GG CGAAGUA UAGGGGCUGA CGCCUGCCCG GUGCUGGAAG GUUAAGAGGA GCGCUUAGCG UAAGCGAAGG UGCGAAUUGA AGC CCCAGU AAACGGCGGC CGUAACUAUA ACGGUCCUAA GGUAGCGAAA UUCCUUGUCG GGUAAGUUCC GACCCGCACG AAAG GCGCA ACGAUCUGGG CACUGUCUCA ACGAGAGACU CGGUGAAAUU AUAGUACCUG UGAAGAUGCA GGUUACCCGC GACAG GACG GAAAGACCCC GUGGAGCUUU ACUGCAGCCU GAUAUUGAAU GUUGGUACAG CUUGUACAGG AUAGGUAGGA GCCUUG GAA ACCGGAGCGC CAGCUUCGGU GGAGGCAUCG GUGGGAUACU ACCCUGGCUG UAUUGACCUU CUAACCCGCC GCCCUUA UC GGGCGGGGAG ACAGUGUCAG GUGGGCAGUU UGACUGGGGC GGUCGCCUCC UAAAAGGUAA CGGAGGCGCC CAAAGGUU C CCUCAGAAUG GUUGGAAAUC AUUCGCAGAG UGUAAAGGCA CAAGGGAGCU UGACUGCGAG ACCUACAAGU CGAGCAGGG ACGAAAGUCG GGCUUAGUGA UCCGGUGGUU CCGCAUGGAA GGGCCAUCGC UCAACGGAUA AAAGCUACCC CGGGGAUAAC AGGCUUAUC UCCCCCAAGA GUCCACAUCG ACGGGGAGGU UUGGCACCUC GAUGUCGGCU CAUCGCAUCC UGGGGCUGUA G UCGGUCCC AAGGGUUGGG CUGUUCGCCC AUUAAAGCGG UACGCGAGCU GGGUUCAGAA CGUCGUGAGA CAGUUCGGUC CC UAUCCGU CGCGGGCGCA GGAAAUUUGA GAGGAGCUGU CCUUAGUACG AGAGGACCGG GAUGGACGCA CCGCUGGUGU ACC AGUUGU UCUGCCAAGG GCAUCGCUGG GUAGCUAUGU GCGGACGGGA UAAGUGCUGA AAGCAUCUAA GCAUGAAGCC CCCC UCAAG AUGAGAUUUC CCAUUCCGCA AGGAAGUAAG AUCCCUGAAA GAUGAUCAGG UUGAUAGGUC UGAGGUGGAA GUGUG GCGA CACAUGGAGC UGACAGAUAC UAAUCGAUCG AGGACUUAAC C

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 36.15752 KDa
SequenceString:
UGGUGGCGAU AGCGAAGAGG UCACACCCGU UCCCAUACCG AACACGGAAG UUAAGCUCUU CAGCGCCGAU GGUAGUCGGG GGUUUCCCC CUGUGAGAGU AGGACGCCGC CAA

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Macromolecule #30: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 30 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 496.854188 KDa
SequenceString: GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC ...String:
GGAGAGUUUG AUCCUGGCUC AGGACGAACG CUGGCGGCGU GCCUAAUACA UGCAAGUCGA GCGGACAGAU GGGAGCUUGC UCCCUGAUG UUAGCGGCGG ACGGGUGAGU AACACGUGGG UAACCUGCCU GUAAGACUGG GAUAACUCCG GGAAACCGGG G CUAAUACC GGAUGGUUGU UUGAACCGCA UGGUUCAAAC AUAAAAGGUG GCUUCGGCUA CCACUUACAG AUGGACCCGC GG CGCAUUA GCUAGUUGGU GAGGUAACGG CUCACCAAGG CGACGAUGCG UAGCCGACCU GAGAGGGUGA UCGGCCACAC UGG GACUGA GACACGGCCC AGACUCCUAC GGGAGGCAGC AGUAGGGAAU CUUCCGCAAU GGACGAAAGU CUGACGGAGC AACG CCGCG UGAGUGAUGA AGGUUUUCGG AUCGUAAAGC UCUGUUGUUA GGGAAGAACA AGUGCCGUUC GAAUAGGGCG GUACC UUGA CGGUACCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAGCGUU GUCCGG AAU UAUUGGGCGU AAAGGGCUCG CAGGCGGUUU CUUAAGUCUG AUGUGAAAGC CCCCGGCUCA ACCGGGGAGG GUCAUUG GA AACUGGGGAA CUUGAGUGCA GAAGAGGAGA GUGGAAUUCC ACGUGUAGCG GUGAAAUGCG UAGAGAUGUG GAGGAACA C CAGUGGCGAA GGCGACUCUC UGGUCUGUAA CUGACGCUGA GGAGCGAAAG CGUGGGGAGC GAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCGCC CCUUAGUGCU GCAGCUAACG CAUUAAGCAC UCCGCCUGG GGAGUACGGU CGCAAGACUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU U AAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUCUGAC AAUCCUAGAG AUAGGACGUC CCCUUCGGGG GC AGAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUG AUCUUA GUUGCCAGCA UUCAGUUGGG CACUCUAAGG UGACUGCCGG UGACAAACCG GAGGAAGGUG GGGAUGACGU CAAA UCAUC AUGCCCCUUA UGACCUGGGC UACACACGUG CUACAAUGGA CAGAACAAAG GGCAGCGAAA CCGCGAGGUU AAGCC AAUC CCACAAAUCU GUUCUCAGUU CGGAUCGCAG UCUGCAACUC GACUGCGUGA AGCUGGAAUC GCUAGUAAUC GCGGAU CAG CAUGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACACCG CCCGUCACAC CACGAGAGUU UGUAACACCC GAAGUCG GU GAGGUAACCU UUUAGGAGCC AGCCGCCGAA GGUGGGACAG AUGAUUGGGG UGAAGUCGUA ACAAGGUAGC CGUAUCGG A AGGUGCGGCU GG

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Macromolecule #50: P-tRNA

MacromoleculeName: P-tRNA / type: rna / ID: 50 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 28.094645 KDa
SequenceString:
GCCGAGGUGG UGGAAUUGGU AGACACGCUA CCUUGAGGUG GUAGUGCCCA AUAGGGCUUA CGGGUUCAAG UCCCGUCCUC GGUACCA

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Macromolecule #52: A/R-tRNA

MacromoleculeName: A/R-tRNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 24.486523 KDa
SequenceString:
GCCCGGAUAG CUCAGUUGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #53: E-tRNA

MacromoleculeName: E-tRNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
Molecular weightTheoretical: 24.140334 KDa
SequenceString:
CCCGGAUAGC UCAGUCGGUA GAGCAGGGGA UUGAAAAUCC CCGUGUCCUU GGUUCGAUUC CGAGUCCGGG CACCA

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 29.674271 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG ...String:
AIKKYKPTSN GRRGMTTSDF AEITTDKPEK SLLAPLHKKG GRNNQGKLTV RHQGGGHKRQ YRVIDFKRDK DGIPGRVATV EYDPNRSAN IALINYADGE KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK GGQLVRSAGT S AQVLGKEG KYVLVRLNSG EVRMILSACR ASIGQVGNEQ HELINIGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRKSPMS PWGKPTLGFK TRKKKNKSDK FIVRR

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 22.375895 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI ...String:
TKGILGRKIG MTQVFAENGD LIPVTVIEAA PNVVLQKKTA ENDGYEAIQL GFDDKREKLS NKPEKGHVAK AETAPKRFVK ELRGVEMDA YEVGQEVKVE IFSAGEIVDV TGVSKGKGFQ GAIKRHGQSR GPMSHGSRYH RRPGSMGPVD PNRVFKGKLL P GRMGGEQI TVQNLEIVKV DAERNLLLIK GNVPGAKKSL ITVKSAVK

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 22.222676 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN ...String:
PKVALYNQNG STAGDIELNA SVFGIEPNES VVFDAILMQR ASLRQGTHKV KNRSEVRGGG RKPWRQKGTG RARQGSIRSP QWRGGGVVF GPTPRSYSYK LPKKVRRLAI KSVLSSKVID NNIIVLEDLT LDTAKTKEMA AILKGLSVEK KALIVTADAN E AVALSARN IPGVTVVEAN GINVLDVVNH EKLLITKAAV EKVEEVL

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 19.789061 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
NRLKEKYNKE IAPALMTKFN YDSVMQVPKI EKIVINMGVG DAVQNAKAID SAVEELTFIA GQKPVVTRAK KSIAGFRLRE GMPIGAKVT LRGERMYDFL DKLISVSLPR VRDFRGVSKK SFDGRGNYTL GIKEQLIFPE IDYDKVTKVR GMDIVIVTTA N TDEEAREL LTQVGMPF

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 19.124855 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
SRVGKKLLEI PSDVTVTLND NNTVAVKGPK GELTRTFHPD MEIKVEDNVL TVARPSDQKE HRALHGTTRS LLGNMVEGVS KGFERGLEL VGVGYRASKS GNKLVLNVGY SHPVEIVPEE GIEIEVPSQT KVVVKGTDKE RVGAIAANIR AVRSPEPYKG K GIRYEGEV VRRKEGK

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Macromolecule #8: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 16.01761 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
TPMANASTIE RKWLVVDAAG KTLGRLSSEV AAILRGKHKP TYTPHVDTGD HVIIINAEKI ELTGKKLTDK IYYRHTQHPG GLKSRTALE MRTNYPEKML ELAIKGMLPK GSLGRQMFKK LNVYRGSEHP HEAQKPEVYE LRG

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Macromolecule #9: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 13.175288 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

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Macromolecule #10: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 15.410694 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

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Macromolecule #11: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 15.320188 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKRE

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Macromolecule #12: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 13.643609 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
SYRKLGRTSA QRKAMLRDLT TDLIINERIE TTETRAKELR SVVEKMITLG KRGDLHARRQ AAAYIRNEVA NEENNQDALQ KLFSDIATR YEERQGGYTR IMKLGPRRGD GAPMAIIELV

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Macromolecule #13: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 12.993829 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

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Macromolecule #14: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 13.416853 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MQKLIEDITK EQLRTDLPAF RPGDTLRVHV KVVEGNRERI QIFEGVVIKR RGGGISETFT VRKISYGVGV ERTFPVHTPK IAKIEVVRY GKVRRAKLYY LRELRGKAAR IKEIRR

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Macromolecule #15: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 13.408813 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
PRVKGGTVTR KRRKKVLKLA KGYFGSKHTL YKVANQQVMK SGNYAFRDRR QKKRDFRKLW ITRINAAARM NGLSYSRLMH GLKLSGIEV NRKMLADLAV NDLTAFNQLA DAAKAQLN

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Macromolecule #16: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 11.164886 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
YAIIKTGGKQ IKVEEGQTVY IEKLAAEAGE TVTFEDVLFV GGDNVKVGNP TVEGATVTAK VEKQGRAKKI TVFRYKPKKN VHKKQGHRQ PYTKVTIEKI NA

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Macromolecule #17: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 12.035066 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
QAKAVARTVR IAPRKARLVM DLIRGKQVGE AVSILNLTPR AASPIIEKVL KSAIANAEHN YEMDANNLVI SQAFVDEGPT LKRFRPRAM GRASQINKRT SHITIVVSEK

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Macromolecule #18: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 10.387095 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
KDPRDVLKRP VITERSADLM TEKKYTFEVD VRANKTEVKD AVESIFGVKV DKVNIMNYKG KSKRVGRYTG MTSRRRKAIV KLTADSKEI E

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Macromolecule #19: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 10.921854 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MHVKKGDKVM VISGKDKGKQ GTILAAFPKK DRVLVEGVNM VKKHSKPTQA NPQGGISNQE APIHVSNVMP LDPKTGEVTR VGYKVEDGK KVRVAKKSGQ VL

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Macromolecule #20: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 8.956143 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSVY PV

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Macromolecule #21: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 6.367546 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
RKCVITGKKT TAGNNRSHAM NASKRTWGAN LQKVRILVNG KPKKVYVSAR ALKSGKVE

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Macromolecule #22: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 7.598849 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAAN

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Macromolecule #23: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 6.519599 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
AKLEITLKRS VIGRPEDQRV TVRTLGLKKT NQTVVHEDNA AIRGMINKVS HLVSVKEQ

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Macromolecule #24: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 6.184386 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
AVPFRRTSKM KKRLRRTHFK LNVPGMTECP SCGEMKLSHR VCKACGSYNG KDIN

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Macromolecule #25: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 5.786696 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRET

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Macromolecule #26: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 5.271332 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

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Macromolecule #27: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 7.320752 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
PKMKTHRGSA KRFKKTGSGK LKRSHAYTSH LFANKSQKQK RKLRKSAVVS AGDFKRIKQM LANI

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Macromolecule #28: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 4.318422 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MKVRPSVKPI CEKCKVIRRK GKVMVICENP KHKQKQG

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Macromolecule #29: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 7.159191 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRQKFA SADGRVDRFN KKY

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Macromolecule #31: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 25.027107 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: LLEAGVHFGH QTRRWNPKMK RYIFTERNGI YIIDLQKTVK KVEEAYNFTK NLAAEGGKIL FVGTKKQAQD SVKEEAQRSG MYYVNQRWL GGTLTNFETI QKRIKRLKDI EKMQENGTFD VLPKKEVVQL KKELERLEKF LGGIKDMKDL PDALFIIDPR K ERIAVAEA ...String:
LLEAGVHFGH QTRRWNPKMK RYIFTERNGI YIIDLQKTVK KVEEAYNFTK NLAAEGGKIL FVGTKKQAQD SVKEEAQRSG MYYVNQRWL GGTLTNFETI QKRIKRLKDI EKMQENGTFD VLPKKEVVQL KKELERLEKF LGGIKDMKDL PDALFIIDPR K ERIAVAEA RKLNIPIIGI VDTNCDPDEI DVVIPANDDA IRAVKLLTSK MADAILEAKQ

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Macromolecule #32: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 23.032318 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: GQKVNPVGLR IGVIRDWESK WYAGKDYADF LHEDLKIREY ISKRLSDASV SKVEIERAAN RVNITIHTAK PGMVIGKGGS EVEALRKAL NSLTGKRVHI NILEIKRADL DAQLVADNIA RQLENRVSFR RAQKQQIQRT MRAGAQGVKT MVSGRLGGAD I ARSEYYSE ...String:
GQKVNPVGLR IGVIRDWESK WYAGKDYADF LHEDLKIREY ISKRLSDASV SKVEIERAAN RVNITIHTAK PGMVIGKGGS EVEALRKAL NSLTGKRVHI NILEIKRADL DAQLVADNIA RQLENRVSFR RAQKQQIQRT MRAGAQGVKT MVSGRLGGAD I ARSEYYSE GTVPLHTLRA DIDYATSEAD TTYGKLGVKV WIYRGEVL

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Macromolecule #33: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 22.250523 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: GPSWKLSRRL GISLSGTGKE LEKRPYAPGP HGPGQRKKLS EYGLQLQEKQ KLRHMYGVNE RQFRTLFDKA GKLAGKHGEN FMILLDSRL DNVVYKLGLA RTRRQARQLV NHGHILVDGS RVDIPSYLVK PGQTIGVREK SRNLSIIKES VEVNNFVPEY L TFDAEKLE ...String:
GPSWKLSRRL GISLSGTGKE LEKRPYAPGP HGPGQRKKLS EYGLQLQEKQ KLRHMYGVNE RQFRTLFDKA GKLAGKHGEN FMILLDSRL DNVVYKLGLA RTRRQARQLV NHGHILVDGS RVDIPSYLVK PGQTIGVREK SRNLSIIKES VEVNNFVPEY L TFDAEKLE GTFTRLPERS ELAPEINEAL IVEFYSR

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Macromolecule #34: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 17.362236 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
RIDPSKLELE ERLVTVNRVA KVVKGGRRFR FAALVVVGDK NGHVGFGTGK AQEVPEAIRK AVEDAKKNLI EVPMVGTTIP HEIIGRFGA GNILLKPASE GTGVIAGGPV RAVLELAGVA DILSKSLGSN TPINMIRATL QGLSELKRAE DVAKLRGKSV E ELLG

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Macromolecule #35: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 10.723045 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
KYEVMYIIRP NIDEESKKAV IERFNNVLTS NGAEITGTKD WGKRRLAYEI NDFRDGFYQI VNVQSDAAAV QEFDRLAKIS DDIIRHIVV KEE

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Macromolecule #36: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 16.885645 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
KGPVAKRDVL PDPIYNSKLV SRLINKMMID GKKGKSQTIL YKSFDIIKER TGNDAMEVFE QALKNIMPVL EVKARRVGGA NYQVPVEVR PERRTTLGLR WLVNYARLRG EKTMEERLAN EILDAANNTG AAVKKREDTH KMAEANKAFA

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Macromolecule #37: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 14.770229 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
VMTDPIADML TRIRNANMVR HEKLEIPASK LKREIAEILK REGFIRDVEF VEDSKQGIIR VFLKYGQNNE RVITGLKRIS KPGLRVYAK SNEVPRVLNG LGIAIISTSQ GVLTDKEARA KQAGGEVLAY VW

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Macromolecule #38: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 13.748775 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
QYYGTGRRKS SVARVRLVPG EGRIVVNNRE ISEHIPSAAL IEDIKQPLTL TETAGTYDVL VNVHGGGLSG QAGAIRHGIA RALLEADPE YRTTLKRAGL LTRDARMKER KKYGLKGARR APQFSK

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Macromolecule #39: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 10.856562 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
IRIRLKAYDH RILDQSAEKI VETAKRSGAS VSGPIPLPTE KSVYTILRAV HKYKDSREQF EMRTHKRLID IVNPTPQTVD ALMRLDLPS GVDIEI

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Macromolecule #40: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 11.946558 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
IESGIAHIRS TFNNTIVTIT DTHGNAISWS SAGALGFRGS RKSTPFAAQM AAETAAKGSI EHGLKTLEVT VKGPGSGREA AIRALQAAG LEVTAIRDVT PVPHNGCRPP KRRRV

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Macromolecule #41: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 14.988358 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
PTINQLIRKG RVSKVENSKS PALNKGYNSF KKEHTNVSSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLT NGIEVTAYIP GIGHNLQEH SVVLIRGGRV KDLPGVRYHI VRGALDTAGV ENRAQGRSKY GTKKPKA

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Macromolecule #42: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 12.3743 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
RIAGVDIPRD KRVVISLTYI FGIGRTTAQQ VLKEAGVSED TRVRDLTEEE LGKIRDIIDK LKVEGDLRRE VSLNIKRLIE IGSYRGIRH RRGLPVRGQN SKNNARTRK

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Macromolecule #43: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 7.132607 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
AKKSMIAKQQ RTPKFKVQEY TRCERCGRPH SVIRKFKLCR ICFRELAYKG QIPGVKKASW

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Macromolecule #44: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 10.124599 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
TQERKNQLIN EFKTHESDTG SPEVQIAILT DSINNLNEHL RTHKKDHHSR RGLLKMVGKR RNLLTYLRNK DVTRYRELIN KLGLR

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Macromolecule #45: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 9.893457 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
AVKIRLKRMG AKKSPFYRIV VADSRSPRDG RFIETVGTYN PVAKPAEVKI DEELALKWLQ TGAKPSDTVR NLFSSQGIME KFHNAKQG

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Macromolecule #46: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 9.863469 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
SERNQRKVYQ GRVVSDKMDK TITVVVETYK KHTLYGKRVK YSKKFKAHDE NNQAKIGDIV KIMETRPLSA TKRFRLVEVV EEAV

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Macromolecule #47: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 7.387742 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
KVCYFTSNGI THIDYKDVDL LKKFVSERGK ILPRRVTGTN AKYQRKLTAA IKRARQMALL PYVS

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Macromolecule #48: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 8.990461 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
SLKKGPFVDG HLMTKIEKLN ETDKKQVVKT WSRRSTIFPQ FIGHTIAVYD GRKHVPVFIS EDMVGHKLGE FAPTRTYK

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Macromolecule #49: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 9.121647 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString:
IKSAIKRTKT NNERRVHNAT IKSAMRTAIK QVEASVANNE ADKAKTALTE AAKRIDKAVK TGLVHKNTAA RYKSRLAKKV NGL

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Macromolecule #51: GTP pyrophosphokinase

MacromoleculeName: GTP pyrophosphokinase / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO / EC number: GTP diphosphokinase
Source (natural)Organism: Bacillus subtilis (strain 168) (bacteria) / Strain: 168
Molecular weightTheoretical: 39.164523 KDa
Recombinant expressionOrganism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
SequenceString: SDMVYVFTPK GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE ILTSKHSYGP SQDWVKLAQT SQAKHKIRQ FFKKQRREEN VEKGRELVEK EIKNLDFELK DVLTPENIQK VADKFNFSNE EDMYAAVGYN GITALQVANR L TEKERKQR ...String:
SDMVYVFTPK GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE ILTSKHSYGP SQDWVKLAQT SQAKHKIRQ FFKKQRREEN VEKGRELVEK EIKNLDFELK DVLTPENIQK VADKFNFSNE EDMYAAVGYN GITALQVANR L TEKERKQR DQEEQEKIVQ EVTGEPKPYP QGRKREAGVR VKGIDNLLVR LSKCCNPVPG DDIVGFITKG RGVSVHREDC PN VKTNEAQ ERLIPVEWEH ESQVQKRKEY NVEIEILGYD RRGLLNEVLQ AVNETKTNIS SVSGKSDRNK VATIHMAIFI QNI NHLHKV VERIKQIRDI YSVRR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 650054

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