+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDGB5 |
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試料 | The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide
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機能・相同性 | 機能・相同性情報 LGI-ADAM interactions / P2Y1 nucleotide receptor binding / beta-1 adrenergic receptor binding / neuroligin family protein binding / NrCAM interactions / positive regulation of neuron projection arborization / regulation of grooming behavior / synaptic vesicle maturation / receptor localization to synapse / cerebellar mossy fiber ...LGI-ADAM interactions / P2Y1 nucleotide receptor binding / beta-1 adrenergic receptor binding / neuroligin family protein binding / NrCAM interactions / positive regulation of neuron projection arborization / regulation of grooming behavior / synaptic vesicle maturation / receptor localization to synapse / cerebellar mossy fiber / cellular response to potassium ion / protein localization to synapse / vocalization behavior / Synaptic adhesion-like molecules / neuron spine / AMPA glutamate receptor clustering / juxtaparanode region of axon / Trafficking of AMPA receptors / dendritic spine morphogenesis / negative regulation of receptor internalization / neuron projection terminus / RHO GTPases activate CIT / postsynaptic neurotransmitter receptor diffusion trapping / Assembly and cell surface presentation of NMDA receptors / acetylcholine receptor binding / establishment or maintenance of epithelial cell apical/basal polarity / Neurexins and neuroligins / neurotransmitter receptor localization to postsynaptic specialization membrane / regulation of NMDA receptor activity / Activation of Ca-permeable Kainate Receptor / cortical cytoskeleton / Signaling by ERBB4 / Negative regulation of NMDA receptor-mediated neuronal transmission / Unblocking of NMDA receptors, glutamate binding and activation / locomotory exploration behavior / social behavior / positive regulation of excitatory postsynaptic potential / AMPA glutamate receptor complex / 長期増強 / neuromuscular process controlling balance / excitatory synapse / positive regulation of protein tyrosine kinase activity / D1 dopamine receptor binding / positive regulation of synaptic transmission / extrinsic component of cytoplasmic side of plasma membrane / ionotropic glutamate receptor binding / Ras activation upon Ca2+ influx through NMDA receptor / dendrite cytoplasm / synaptic membrane / 学習 / PDZ domain binding / postsynaptic density membrane / 接着結合 / regulation of long-term neuronal synaptic plasticity / neuromuscular junction / establishment of protein localization / kinase binding / 細胞接着 / endocytic vesicle membrane / シナプス小胞 / 細胞結合 / nervous system development / positive regulation of cytosolic calcium ion concentration / RAF/MAP kinase cascade / scaffold protein binding / chemical synaptic transmission / basolateral plasma membrane / postsynaptic membrane / protein phosphatase binding / protein-containing complex assembly / 樹状突起スパイン / postsynaptic density / neuron projection / glutamatergic synapse / シナプス / protein-containing complex binding / 小胞体 / シグナル伝達 / 細胞膜 / 細胞質基質 / 細胞質 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | 日付: 2019 Sep 19 タイトル: How the dual PDZ domain from Postsynaptic density protein 95 clusters ion channels and receptors 著者: Rodzli N / Lockhart-Cairns M / Levy C / Chipperfield J / Bird L / Baldock C |
登録者 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #3709 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #3710 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3711 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3712 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3713 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3714 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3715 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3716 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3717 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
モデル #3718 | タイプ: atomic / カイ2乗値: 8.74 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide 試料濃度: 15 mg/ml |
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バッファ | 名称: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM RRESEI / pH: 8.5 / コメント: standard buffer plus 10mM ligand peptide |
要素 #1889 | 名称: PDZ1-2 / タイプ: protein / 記述: PDZ1-2 fragment of PSD-95/Disks large homolog 4 / 分子量: 20.8 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P78352 配列: GPGTEGEMEY EEITLERGNS GLGFSIAGGT DNPHIGDDPS IFITKIIPGG AAAQDGRLRV NDSILFVNEV DVREVTHSAA VEALKEAGSI VRLYVMRRKP PAEKVMEIKL IKGPKGLGFS IAGGVGNQHI PGDNSIYVTK IIEGGAAHKD GRLQIGDKIL AVNSVGLEDV ...配列: GPGTEGEMEY EEITLERGNS GLGFSIAGGT DNPHIGDDPS IFITKIIPGG AAAQDGRLRV NDSILFVNEV DVREVTHSAA VEALKEAGSI VRLYVMRRKP PAEKVMEIKL IKGPKGLGFS IAGGVGNQHI PGDNSIYVTK IIEGGAAHKD GRLQIGDKIL AVNSVGLEDV MHEDAVAALK NTYDVVYLKV AKPSNA |
-実験情報
ビーム | 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotronシンクロトロン / 波長: 0.123987 Å / スペクトロメータ・検出器間距離: 3 mm | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||
スキャン | タイトル: The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) bound to RRESEI peptide 測定日: 2015年6月11日 / 保管温度: 15 °C / セル温度: 15 °C / 照射時間: 0.05 sec. / フレーム数: 15 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 726 /
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結果 | カーブのタイプ: single_conc コメント: Scattering data are fitted using the ATSAS OLIGOMER program using a suite of models. The ATSAS program FFMAKER was used to generate form factors for each model in the suite. Each model ...コメント: Scattering data are fitted using the ATSAS OLIGOMER program using a suite of models. The ATSAS program FFMAKER was used to generate form factors for each model in the suite. Each model is assigned a volume fraction to fit the observed scattering profile. 3 monomer models are included, the first is a compact conformation of PDZ1-2 similar to PDB entries 6spv/6spz; the other two are domain models obtained from a representative run of the ATSAS EOM program with data projected to infinite dilution. Multimeric models are drawn from a "clustering Spacegroup", unit cell 14.8nm symmetry I2(1)3, and consist of identical copies of an extended conformation of PDZ1-2 (similar to PDB entry 3zrt) assembled by symmetry operations. In the order of deposition: Model number; Stoichiometry; MW (kDa); source; Volume fraction. 1; 1; 21; Crystal Structure; 0.112. 2; 1; 21; EOM; 0.186. 3; 1; 21; EOM; 0.656. 4; 2; 42; clustering Spacegroup; 0.000. 5; 4; 84; clustering Spacegroup; 0.000. 6; 8; 168; clustering Spacegroup; 0.034. 7; 12, 252; clustering Spacegroup; 0.005. 8; 16, 336, clustering Spacegroup; 0.000. 9; 20, 420, clustering Spacegroup; 0.000. 10; 24, 504, clustering Spacegroup; 0.006.
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