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- SASDA99: Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDA99
SampleComplex between ovine IL-2 and GM-CSF/IL-2 inhibition factor
  • GM-CSF/IL-2 inhibition factor (protein), Orf virus
  • Interleukin-2Interleukin 2 (protein), Ovis aries
Function / homology
Function and homology information


interleukin-2 receptor binding / positive regulation of plasma cell differentiation / leukocyte activation involved in immune response / negative regulation of B cell apoptotic process / positive regulation of immunoglobulin production / positive regulation of activated T cell proliferation / positive regulation of interleukin-17 production / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity ...interleukin-2 receptor binding / positive regulation of plasma cell differentiation / leukocyte activation involved in immune response / negative regulation of B cell apoptotic process / positive regulation of immunoglobulin production / positive regulation of activated T cell proliferation / positive regulation of interleukin-17 production / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity / growth factor activity / adaptive immune response / extracellular space
Similarity search - Function
Poxvirus chemokine inhibitor superfamily / Interleukin-2 / Interleukin-2, conserved site / Interleukin 2 / Interleukin-2 signature. / Interleukin-2 family / Four-helical cytokine-like, core
Similarity search - Domain/homology
Interleukin-2 / GM-CSF/IL-2 inhibition factor
Similarity search - Component
Biological speciesOrf virus
Ovis aries (sheep)
CitationDate: 2016 Nov 7
Title: Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF
Authors: Felix J / Kandiah E / De Munck S / Bloch Y / van Zundert G / Pauwels K / Dansercoer A / Novanska K / Read R / Bonvin A / Vergauwen B / Verstraete K / Gutsche I
Contact author
  • Jan Felix

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #550
Type: mix / Software: FoxS / Radius of dummy atoms: 1.90 A / Chi-square value: 35.9502544171
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor
Purity method: SEC-SAXS / Specimen concentration: 8.8 mg/ml / Entity id: 209 / 212
BufferName: HEPES / Concentration: 20.00 mM / pH: 7.4 / Composition: 150 mM NaCl
Entity #209Type: protein / Description: GM-CSF/IL-2 inhibition factor / Formula weight: 29.924 / Num. of mol.: 4 / Source: Orf virus / References: UniProt: Q9J5U5
Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID ...Sequence:
MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID YPHTGMEFLQ HVLRPNRRFA PAKLHMDLEV DHRCVSAVHV KAFLQDACSA RKARTPLYFA GHGCNHPDRR PKNPVPRPQH VSSPISRKCS MQTAR
Entity #212Type: protein / Description: Interleukin-2Interleukin 2 / Formula weight: 15.56 / Num. of mol.: 1 / Source: Ovis aries / References: UniProt: P19114
Sequence:
APTSSSTGNT MKEVKSLLLD LQLLLEKVKN PENLKLSRMH TFNFYMPKVN ATELKHLKCL LEELKLLEEV LDLAPSKNLN TREIKDSMDN IKRIVLELQG SETRFTCEYD DATVKAVEFL NKWITFCQSI YSTMT

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm
DetectorName: AVIEX / Type: CCD
Scan
Title: complex between ovine IL-2 and GM-CSF/IL-2 inhibit / Measurement date: Sep 10, 2014 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 12 / Unit: 1/nm /
MinMax
Q0.0736 6.1398
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 472 /
MinMax
Q0.0118853 0.278238
P(R) point1 472
R0 129
Result
Type of curve: other
Comments: Modeling of SAXS data: A model of the GIF:IL-2 complex, based on the fit in a low-resolution (24 Å) map obtained by Negative-Stain Electron Microscopy, was used as an input for the ...Comments: Modeling of SAXS data: A model of the GIF:IL-2 complex, based on the fit in a low-resolution (24 Å) map obtained by Negative-Stain Electron Microscopy, was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked glycosylation. During each Allosmod-FoXS run, data up to a scattering angle of 0.5 Å-1 was used. Fits to the experimental SAXS data were calculated using the FoXS software. Note that the exact orientation of IL-2 is uncertain due to the low resolution of the EM map.
ExperimentalStandardPorod
MW155 kDa155 kDa158 kDa
Volume--253 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.09156 2.0E-5 0.092 -
Radius of gyration, Rg4.08 nm0.001 4.05 nm0.01

MinMax
D-12.9
Guinier point9 44

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