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Open data
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Basic information
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Title | Cryo-EM structure of Bacillus megaterium gas vesicles | |||||||||
![]() | Sharpened cryo-EM density - cut to 128px box size around the part optimized in local refinement | |||||||||
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Function / homology | ![]() gas vesicle shell / vesicle membrane / ![]() Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / ![]() | |||||||||
![]() | Huber ST / Evers W / Jakobi AJ | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Cryo-EM structure of gas vesicles for buoyancy-controlled motility. Authors: Stefan T Huber / Dion Terwiel / Wiel H Evers / David Maresca / Arjen J Jakobi / ![]() Abstract: Gas vesicles are gas-filled nanocompartments that allow a diverse group of bacteria and archaea to control their buoyancy. The molecular basis of their properties and assembly remains unclear. Here, ...Gas vesicles are gas-filled nanocompartments that allow a diverse group of bacteria and archaea to control their buoyancy. The molecular basis of their properties and assembly remains unclear. Here, we report the 3.2 Å cryo-EM structure of the gas vesicle shell made from the structural protein GvpA that self-assembles into hollow helical cylinders closed off by cone-shaped tips. Two helical half shells connect through a characteristic arrangement of GvpA monomers, suggesting a mechanism of gas vesicle biogenesis. The fold of GvpA features a corrugated wall structure typical for force-bearing thin-walled cylinders. Small pores enable gas molecules to diffuse across the shell, while the exceptionally hydrophobic interior surface effectively repels water. Comparative structural analysis confirms the evolutionary conservation of gas vesicle assemblies and demonstrates molecular features of shell reinforcement by GvpC. Our findings will further research into gas vesicle biology and facilitate molecular engineering of gas vesicles for ultrasound imaging. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 7.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 23.1 KB 23.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 17.7 KB | Display | ![]() |
Images | ![]() | 245.5 KB | ||
Masks | ![]() | 8 MB | ![]() | |
Others | ![]() ![]() ![]() ![]() | 115.6 MB 7.5 MB 7.5 MB 7.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7r1cMC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Sharpened cryo-EM density - cut to 128px box size around the part optimized in local refinement | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.37 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Symmetrized cryo-EM density before local refinement / helical...
File | emd_14238_additional_1.map | ||||||||||||
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Annotation | Symmetrized cryo-EM density before local refinement / helical twist is -3.874 degrees / helical rise is 0.525 Angstroms. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Unsharpened cryo-EM density - cut to 128px box...
File | emd_14238_additional_2.map | ||||||||||||
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Annotation | Unsharpened cryo-EM density - cut to 128px box size around the part optimized in local refinement | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map B - cut to 128px box size...
File | emd_14238_half_map_1.map | ||||||||||||
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Annotation | Half-map B - cut to 128px box size around the part optimized in local refinement | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map A - cut to 128px box size...
File | emd_14238_half_map_2.map | ||||||||||||
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Annotation | Half-map A - cut to 128px box size around the part optimized in local refinement | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Helical assembly of GvpB monomers forming the gas vesicle wall
Entire | Name: Helical assembly of GvpB monomers forming the gas vesicle wall |
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Components |
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-Supramolecule #1: Helical assembly of GvpB monomers forming the gas vesicle wall
Supramolecule | Name: Helical assembly of GvpB monomers forming the gas vesicle wall type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 9.99 MDa |
-Macromolecule #1: Gas vesicle structural protein
Macromolecule | Name: Gas vesicle structural protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() Strain: ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512 |
Molecular weight | Theoretical: 9.62685 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MSIQKSTNSS SLAEVIDRIL DKGIVIDAFA RVSVVGIEIL TIEARVVIAS VDTWLRYAEA VGLLRDDVEE NGLPERSNSS EGQPRFSI |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | helical reconstruction |
Aggregation state | helical array |
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Sample preparation
Concentration | 0.45 mg/mL | |||||||||
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Buffer | pH: 8 Component:
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Grid | Model: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 293 K / Instrument: LEICA PLUNGER / Details: Blot times between 5 and 11 seconds.. | |||||||||
Details | Concentration measured by OD(500)=3.12 against a sonicated blank. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 4351 / Average exposure time: 2.4 sec. / Average electron dose: 30.0 e/Å2 Details: One shot per hole 1.37 A/pix 60 fractions over 30 e-/A2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-7r1c: |