ジャーナル: Structure / 年: 2005 タイトル: Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution. 著者: Xing Zhang / Yongchang Ji / Lan Zhang / Stephen C Harrison / Dan C Marinescu / Max L Nibert / Timothy S Baker / 要旨: Reovirus is a useful model for addressing the molecular basis of membrane penetration by one of the larger nonenveloped animal viruses. We now report the structure of the reovirus virion at ...Reovirus is a useful model for addressing the molecular basis of membrane penetration by one of the larger nonenveloped animal viruses. We now report the structure of the reovirus virion at approximately 7.0 A resolution as obtained by electron cryomicroscopy and three-dimensional image reconstruction. Several features of the myristoylated outer capsid protein mu1, not seen in a previous X-ray crystal structure of the mu1-sigma3 heterohexamer, are evident in the virion. These features appear to be important for stabilizing the outer capsid, regulating the conformational changes in mu1 that accompany perforation of target membranes, and contributing directly to membrane penetration during cell entry.
A: major outer-capsid protein mu1 B: major outer-capsid protein mu1 C: major outer-capsid protein mu1 S: major capsid surface protein sigma-3 D: major capsid surface protein sigma-3 E: major capsid surface protein sigma-3 F: major capsid surface protein sigma-3 M: major capsid surface protein sigma-3 N: major capsid surface protein sigma-3 O: major capsid surface protein sigma-3 G: major capsid surface protein sigma-3 H: major capsid surface protein sigma-3 I: major capsid surface protein sigma-3 P: major outer-capsid protein mu1 Q: major outer-capsid protein mu1 R: major outer-capsid protein mu1 J: major outer-capsid protein mu1 K: major outer-capsid protein mu1 L: major outer-capsid protein mu1 T: major outer-capsid protein mu1 U: guanylyltransferase V: major core protein lambda 1 W: major core protein lambda 1 X: Sigma 2 protein Y: Sigma 2 protein Z: Sigma 2 protein 1: Minor core protein lambda 3
A: major outer-capsid protein mu1 B: major outer-capsid protein mu1 C: major outer-capsid protein mu1 S: major capsid surface protein sigma-3 D: major capsid surface protein sigma-3 E: major capsid surface protein sigma-3 F: major capsid surface protein sigma-3 M: major capsid surface protein sigma-3 N: major capsid surface protein sigma-3 O: major capsid surface protein sigma-3 G: major capsid surface protein sigma-3 H: major capsid surface protein sigma-3 I: major capsid surface protein sigma-3 P: major outer-capsid protein mu1 Q: major outer-capsid protein mu1 R: major outer-capsid protein mu1 J: major outer-capsid protein mu1 K: major outer-capsid protein mu1 L: major outer-capsid protein mu1 T: major outer-capsid protein mu1 U: guanylyltransferase V: major core protein lambda 1 W: major core protein lambda 1 X: Sigma 2 protein Y: Sigma 2 protein Z: Sigma 2 protein 1: Minor core protein lambda 3
A: major outer-capsid protein mu1 B: major outer-capsid protein mu1 C: major outer-capsid protein mu1 S: major capsid surface protein sigma-3 D: major capsid surface protein sigma-3 E: major capsid surface protein sigma-3 F: major capsid surface protein sigma-3 M: major capsid surface protein sigma-3 N: major capsid surface protein sigma-3 O: major capsid surface protein sigma-3 G: major capsid surface protein sigma-3 H: major capsid surface protein sigma-3 I: major capsid surface protein sigma-3 P: major outer-capsid protein mu1 Q: major outer-capsid protein mu1 R: major outer-capsid protein mu1 J: major outer-capsid protein mu1 K: major outer-capsid protein mu1 L: major outer-capsid protein mu1 T: major outer-capsid protein mu1 U: guanylyltransferase V: major core protein lambda 1 W: major core protein lambda 1 X: Sigma 2 protein Y: Sigma 2 protein Z: Sigma 2 protein 1: Minor core protein lambda 3
x 5
icosahedral pentamer
9.44 MDa, 135 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
9,439,629
135
ポリマ-
9,439,629
135
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: major outer-capsid protein mu1 B: major outer-capsid protein mu1 C: major outer-capsid protein mu1 S: major capsid surface protein sigma-3 D: major capsid surface protein sigma-3 E: major capsid surface protein sigma-3 F: major capsid surface protein sigma-3 M: major capsid surface protein sigma-3 N: major capsid surface protein sigma-3 O: major capsid surface protein sigma-3 G: major capsid surface protein sigma-3 H: major capsid surface protein sigma-3 I: major capsid surface protein sigma-3 P: major outer-capsid protein mu1 Q: major outer-capsid protein mu1 R: major outer-capsid protein mu1 J: major outer-capsid protein mu1 K: major outer-capsid protein mu1 L: major outer-capsid protein mu1 T: major outer-capsid protein mu1 U: guanylyltransferase V: major core protein lambda 1 W: major core protein lambda 1 X: Sigma 2 protein Y: Sigma 2 protein Z: Sigma 2 protein 1: Minor core protein lambda 3
x 6
icosahedral 23 hexamer
11.3 MDa, 162 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
11,327,554
162
ポリマ-
11,327,554
162
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
5
5
登録構造と同一(異なる座標系)
icosahedral asymmetric unit, std point frame
タイプ
名称
対称操作
数
transform to point frame
1
対称性
点対称性: (ヘルマン・モーガン記号: 532 / シェーンフリース記号: I (正20面体型対称))