Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure and design of Langya virus glycoprotein antigens.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 121, Issue 16, Page e2314990121, Year 2024
Publish date	Apr 16, 2024
Authors	Zhaoqian Wang / Matthew McCallum / Lianying Yan / Cecily A Gibson / William Sharkey / Young-Jun Park / Ha V Dang / Moushimi Amaya / Ashley Person / Christopher C Broder / David Veesler /
PubMed Abstract	Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which ...Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which are the main targets of neutralizing antibodies. We show here that the LayV F and G glycoproteins promote membrane fusion with human, mouse, and hamster target cells using a different, yet unknown, receptor than Nipah virus (NiV) and Hendra virus (HeV) and that NiV- and HeV-elicited monoclonal and polyclonal antibodies do not cross-react with LayV F and G. We determined cryoelectron microscopy structures of LayV F, in the prefusion and postfusion states, and of LayV G, revealing their conformational landscape and distinct antigenicity relative to NiV and HeV. We computationally designed stabilized LayV G constructs and demonstrate the generalizability of an HNV F prefusion-stabilization strategy. Our data will support the development of vaccines and therapeutics against LayV and closely related HNVs.
External links	Proc Natl Acad Sci U S A / PubMed:38593070 / PubMed Central
Methods	EM (single particle)
Resolution	2.8 - 3.9 Å
Structure data	41636 8tvb EMDB-41636, PDB-8tvb: Ghanaian virus fusion glycoprotein (GhV F) Method: EM (single particle) / Resolution: 2.9 Å 41639 8tve EMDB-41639, PDB-8tve: Langya henipavirus fusion protein in postfusion state Method: EM (single particle) / Resolution: 3.9 Å 41640 8tvf EMDB-41640, PDB-8tvf: Langya henipavirus fusion protein in prefusion state Method: EM (single particle) / Resolution: 2.8 Å 41641 8tvg EMDB-41641, PDB-8tvg: Langya henipavirus postfusion F protein in complex with the 4G5 Fab, local refinement of the viral membrane distal region Method: EM (single particle) / Resolution: 3.3 Å 41642 8tvh EMDB-41642, PDB-8tvh: Langya henipavirus postfusion F protein in complex with 4G5 Fab, local refinement of the viral membrane proximal region Method: EM (single particle) / Resolution: 3.6 Å 41643 8tvi EMDB-41643, PDB-8tvi: Langya Virus G glycoprotein (LayV G) with stabilizing mutations Method: EM (single particle) / Resolution: 3.2 Å EMDB-41644: Langya henipavirus postfusion fusion protein in complex with 4G5 Fab (global refinement) Method: EM (single particle) / Resolution: 3.5 Å 43593 8vwp EMDB-43593, PDB-8vwp: Langya Virus attachment (G) glycoprotein with K85L/L86K mutation Method: EM (single particle) / Resolution: 3.21 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine ChemComp-HOH: WATER / Water ChemComp-ZN: Unknown entry
Source	henipavirus ghanaense thermotoga maritima msb8 (bacteria) langya virus mus musculus (house mouse) Mus sp. (mice)
Keywords	VIRAL PROTEIN / GhV F / Glycoprotein / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID / Inhibitor / VIRAL PROTEIN-IMMUNE SYSTEM complex / Langya / henipavirus / fusion protein / postfusion / LayVF / prefusion / VIRAL PROTEIN/IMMUNE SYSTEM / LayVG / LayV / G protein / attachment protein / attachment glycoprotein / Langya virus / protein design / protein stabilization / attachment